CN-122012235-A - Biological 3D printing visual craniocerebral tumor drug in-vitro screening model device and application thereof

CN122012235ACN 122012235 ACN122012235 ACN 122012235ACN-122012235-A

Abstract

The invention belongs to the technical field of biological equipment, and particularly relates to a biological 3D printing visual craniocerebral tumor drug in-vitro screening model device and application thereof, wherein the visual craniocerebral tumor drug in-vitro screening model device mainly comprises a liquid inlet, a three-way valve, a bionic blood brain barrier model, a tumor model culture area, a culture container, a container cover, a cell culture bag, a camera and a cell activity detection electrode; the invention integrates the construction of the in-vitro bionic blood brain barrier model and the construction of the tumor model, and can be used for assisting in evaluating the type of craniocerebral tumor treatment drugs and the screening and evaluating application of drug dosage. The invention solves the visualization of dynamic progress and drug screening in the rapid growth process of tumor cells, and a stable and high-flux drug screening chip is formed preliminarily through the real-time monitoring of the cell activity detection electrode, thereby providing an auxiliary basis for the clinical treatment research of craniocerebral tumors.

Inventors

GONG JIN
LIANG CHAOFENG
LI MANTING
Wan Chengjie
GUO SHUANGZHUANG

Assignees

中山大学附属第三医院
中山大学

Dates

Publication Date: 20260512
Application Date: 20260104

Claims (5)

1. The biological 3D printing visualization craniocerebral tumor drug in-vitro screening model device is characterized by comprising a liquid inlet module, a bionic blood brain barrier module, a tumor culture module and a liquid outlet module which are sequentially communicated; the bionic blood brain barrier module is constructed by adopting a biological 3D printing technology; The tumor culture module comprises a culture container, a cell culture bag arranged in the culture container, and a camera and a cell activity detection electrode arranged on a container cover.
2. The biological 3D printing visualized craniocerebral tumor drug in-vitro screening model device of claim 1, wherein a filter string bag is arranged in the cell culture bag.
3. The biological 3D printing visualized craniocerebral tumor drug in-vitro screening model device of claim 1, wherein the bionic blood brain barrier module is formed by sequentially printing a brain microvascular endothelial cell layer, a pericyte layer and an astrocyte layer.
4. The biological 3D printing visualized craniocerebral tumor drug in-vitro screening model device of claim 1, wherein the liquid inlet module and the liquid outlet module are respectively provided with a three-way valve, and a heparin cap is arranged at an external port of the three-way valve.
5. Use of a biological 3D printing visualization craniocerebral tumour drug in vitro screening model device according to any one of claims 1 to 4 in craniocerebral tumour drug screening.

Description

Biological 3D printing visual craniocerebral tumor drug in-vitro screening model device and application thereof Technical Field The invention belongs to the technical field of biological equipment, and particularly relates to a biological 3D printing visual craniocerebral tumor drug in-vitro screening model device and application thereof. Background Craniocerebral tumors can have serious influence on the nervous system function of patients, especially the malignant brain tumor grows rapidly, and various operation radiotherapy and chemotherapy, targeted therapy and immunotherapy technologies are gradually developed at present, but are not effectively controlled yet. Intracranial reasons are a special cause of blood brain/tumor barrier, greatly limiting the ability of chemical drugs, cellular preparations, to enter intracranial tumor tissue. In the prior art, a plurality of reports are carried out on the construction of a bionic blood brain barrier model, then, less in vitro construction is carried out on the bionic blood brain/tumor barrier model, especially, the growth of tumors is rapid, and the progress needs to be observed in real time, so that a visual craniocerebral tumor drug in vitro screening model device needs to be developed. Disclosure of Invention In order to solve the technical problems, the invention provides a biological 3D printing visual craniocerebral tumor drug in-vitro screening model device and application thereof, which can simulate the interaction relationship between a blood brain barrier model and intracranial tumor and provide convenience for research and development of intracranial tumor drug screening. An in vitro screening model device for biological 3D printing visualization of craniocerebral tumor drugs, comprising: the bionic blood brain barrier model is built by adopting a biological 3D printing technology in a layered mode and comprises a brain microvascular endothelial cell layer, a pericyte layer and an astrocyte layer, a tumor model culture area comprises a cell culture bag for culturing tumor cells, tissues or organoids and is provided with a camera and a cell activity detection electrode, a culture container is provided with a left interface and a right interface which are communicated with a system pipeline, a container cover is a detachable rotary cover and is provided with an air-permeable bacterial filter membrane and an electrode/camera jack, the liquid outlet system is provided with a three-way valve and a liquid outlet which are used for sampling or discharging liquid, and all parts in the device are connected through pipelines to form a closed circulation or unidirectional flow system. Further, a filter net bag is arranged in the cell culture bag to prevent cells from escaping; The camera is connected with the high-definition recording system to realize real-time dynamic observation; the external port of the three-way valve is provided with a heparin cap, so that injection or sampling is facilitated. The beneficial effects that can be achieved are as follows, Integrating the blood brain barrier and the tumor model to more truly simulate the intracranial drug delivery process; Support multiple types of screening such as drug permeability, dose effect, immune cell delivery, etc.; the system has the functions of real-time monitoring and visualization, and improves screening efficiency and data reliability; The structure is modularized, so that high-flux expansion and standardization operation are facilitated. Further, the invention provides a biological 3D printing visual craniocerebral tumor drug in-vitro screening model device, which comprises a system pipeline, wherein the structure of the system pipeline comprises a liquid inlet, a three-way valve, a bionic blood brain barrier model, a tumor model culture area, a cell culture bag, a container cover, a cell viability detection electrode, a camera, a three-way valve and a liquid outlet; The liquid inlet and the liquid outlet are provided with detachable sealing covers; the liquid inlet and the liquid outlet are internally provided with bacterial filtering films; Two three-way valves at the head end and the tail end are arranged in the system pipeline; The three-way valve can be used for liquid injection or liquid taking by using a syringe; the liquid injection can be one or two or more of a medicine-containing solution, a cell-containing solution and a nutrient-containing solution; The external ports of the three-way valve are provided with detachable sealing covers; The sealing cover is provided with a heparin cap; the bionic blood brain barrier model and the tumor organoid model are sequentially printed in a biological 3D printing mode; The printing material related to the biological 3D printing mode can be one or two or more of acellular matrix, natural biological material and artificial synthetic material; the biological 3D printed cells adopted by the bionic blood brain barrier are brain microvascular endot