CN-122012243-A - Tough mycelium structure and culture method thereof

CN122012243ACN 122012243 ACN122012243 ACN 122012243ACN-122012243-A

Abstract

A tough mycelium structure and a culture method thereof are provided, wherein the tough mycelium structure comprises a porous hollow support carrier, culture medium cloth and a mycelium layer. The porous hollow support carrier comprises a support framework and a plurality of carrier holes penetrating through the support framework. The culture medium is arranged on the porous hollow support carrier and comprises biomass type nutrition fibers and a plurality of fiber holes penetrating through the biomass type nutrition fibers, wherein the biomass type nutrition fibers are completely laid on the porous hollow support carrier. The mycelium layer is arranged in the carrier holes and the fiber holes in a penetrating way in a surrounding way, extends up and down and completely coats the porous hollow support carrier and the culture medium cloth, wherein the diameter of each carrier hole is 10-1000 microns and is larger than that of each fiber hole. Also provides a culture method of the tough mycelium structure. By adopting the porous hollow support carrier as the bottom support structure, the uniformity of mycelium growth can be improved. The adoption of the porous hollow support carrier and the culture medium cloth can improve the softness, the structural strength and the ductility of the tough mycelium structure.

Inventors

ZHOU XINYING
ZHANG HAOZHE
LI ZEWEI
XU HUIRU

Assignees

财团法人纺织产业综合研究所

Dates

Publication Date: 20260512
Application Date: 20250801
Priority Date: 20241106

Claims (10)

1. A tough mycelium structure comprising: the porous hollow support carrier comprises a support framework and a plurality of carrier holes penetrating through the support framework; A culture medium cloth arranged on the porous hollow support carrier and comprising a biomass type nutrition fiber completely laid on the porous hollow support carrier and a plurality of fiber holes passing through the biomass type nutrition fiber, and The mycelium layer is arranged in the carrier holes and the fiber holes in a penetrating way, extends up and down and completely covers the porous hollowed-out supporting carrier and the culture medium cloth, wherein the diameter of each carrier hole is 10-1000 microns and is larger than that of each fiber hole.
2. The tough mycelium structure of claim 1, wherein the material of the medium cloth and the porous hollow support carrier is sterilized at 115 ℃ for 30 minutes, and the structure remains unchanged.
3. The tenacious mycelium structure of claim 1, wherein the mycelium of the mycelium layer comprises fungal mycelium of the genus Polyporus.
4. The tenacious mycelium structure of claim 1, wherein the biomass-based nutrient fiber comprises a plurality of nutrient fibers, each of the nutrient fibers being staggered, wherein the plurality of nutrient fibers comprises a plant-derived protein, a carbohydrate, an inorganic salt, a vitamin, or a combination thereof.
5. A method for culturing a tough mycelium structure, comprising: Mixing, namely mixing a framework material and a nutritional material to obtain a spinning solution; a wet spinning step, namely, carrying out a wet spinning process on the spinning solution to obtain a culture medium cloth, wherein the culture medium cloth comprises biomass type nutrition fibers and a plurality of fiber holes penetrating through the biomass type nutrition fibers; Erecting a stacked structure, namely fully laying the culture medium cloth on a porous hollow support carrier to form the stacked structure, wherein the porous hollow support carrier comprises a support framework and a plurality of carrier holes penetrating through the support framework, and the diameter of each carrier hole is 10-1000 microns and is larger than that of each fiber hole; A bacterial liquid adding step of adding bacterial liquid containing mycelium onto the culture medium cloth in the stacked structure, and And a mycelium growing step, namely placing the stacked structure in a specific temperature for culture, so that the mycelium grows on the culture medium cloth, and surrounds and penetrates through the plurality of carrier holes and the plurality of fiber holes, extends up and down and coats the porous hollow support carrier and the culture medium cloth, thereby obtaining a tough mycelium structure.
6. The method according to claim 5, wherein in the step of adding the bacterial liquid, 3.5X10 -3 mg to 5X 10 -2 mg of the mycelia are added per cubic centimeter of the culture medium cloth.
7. The method of culturing a tough mycelium structure according to claim 5, wherein the specific temperature is 22 ℃ to 30 ℃.
8. The method of claim 5, wherein the mycelium comprises a fungal mycelium of Pleurotus eryngii, oyster mushroom, ganoderma lucidum, schizophyllum commune, coriolus versicolor, agaricus campestris, or a combination thereof.
9. The method of culturing a tough mycelium structure according to claim 5, wherein in the mycelium growth step, air in each direction is circulated so that the growth direction of the mycelium extends at least in both directions.
10. The method of claim 5, wherein the mycelium of the tenacious mycelium structure has a mycelium diameter of 0.5 micrometers to 1 micrometer.

Description

Tough mycelium structure and culture method thereof Technical Field The present disclosure relates to tough mycelium structures and methods of culturing the same. Background Along with the improvement of the standard of living, the requirements of people on leather are higher and higher, wherein the application of mycelium skin gradually breaks through the limitation of dermis, and the mycelium skin is widely applied to various industrial fields. Hyphal skin made of hypha has advantages of beauty and soft touch as compared with vegetable skin made of plant, and is thus popular in the market. The existing hypha leather technology comprises a coating method (using chemical adhesive hypha and fiber layers), a liquid culture method (culture solution culture), a solid culture method (culture medium culture) and other modes, but the finished product has the limitations of insufficient structural strength and poor flexibility. Therefore, it is a problem to be solved how to provide a tough mycelium structure that enhances structural strength as well as flexibility. Disclosure of Invention The present disclosure provides a tough mycelium structure and a cultivation method of the tough mycelium structure, which can promote uniformity of mycelium growth by adopting a porous hollow support carrier as a bottom support structure and use a culture medium cloth as a growth substrate of mycelium, meanwhile, the porous hollow support carrier and the culture medium cloth both contain reserved holes, keep air circulation, and enable the mycelium to completely wrap the porous hollow support carrier and the culture medium cloth through the holes and coil for growth, thereby obtaining the tough mycelium structure with improved softness, structural strength and ductility. Some embodiments of the present disclosure provide a tough mycelium structure comprising a porous hollowed-out support carrier, a culture medium cloth, and a mycelium layer. The porous hollow support carrier comprises a support framework and a plurality of carrier holes penetrating through the support framework. The culture medium is arranged on the porous hollow support carrier and comprises biomass type nutrition fibers and a plurality of fiber holes penetrating through the biomass type nutrition fibers, wherein the biomass type nutrition fibers are completely laid on the porous hollow support carrier. The mycelium layer is arranged in the carrier holes and the fiber holes in a penetrating way in a surrounding way, extends up and down and completely coats the porous hollow support carrier and the culture medium cloth, wherein the diameter of each carrier hole is 10-1000 microns and is larger than that of each fiber hole. According to one embodiment of the present disclosure, the material of the culture medium cloth and the porous hollow support carrier is sterilized at 115 ℃ for 30 minutes, and the structure is maintained unchanged. According to one embodiment of the present disclosure, the mycelium of the mycelium layer comprises fungal mycelium of the genus Polyporus. According to one embodiment of the present disclosure, the cellulosic nutritional fiber comprises a plurality of nutritional fibers, each nutritional fiber being staggered, wherein the nutritional fibers comprise plant-derived proteins, carbohydrates, inorganic salts, vitamins, or combinations thereof. Some embodiments of the present disclosure provide a method for culturing a tough mycelium structure, comprising a mixing step of mixing a skeleton material and a nutritional material to obtain a spinning solution, a wet spinning step of performing a wet spinning process on the spinning solution to obtain a culture medium cloth, wherein the culture medium cloth comprises a biomass type nutritional fiber and a plurality of fiber holes penetrating the biomass type nutritional fiber, a stacking structure erecting step of completely paving the culture medium cloth on a porous hollow support carrier to form a stacking structure, wherein the porous hollow support carrier comprises a support skeleton and a plurality of carrier holes penetrating the support skeleton, the diameter of each carrier hole is 10-1000 microns and is larger than that of each fiber hole, a mycelium liquid adding step of adding a mycelium liquid containing mycelium onto the culture medium cloth in the stacking structure, and a mycelium growing step of placing the stacking structure in a specific temperature for culturing so that the mycelium grows on the culture medium cloth, encircling the carrier holes and the fiber holes, extending up and down and coating the porous hollow support carrier and the culture medium cloth, thereby obtaining the tough mycelium structure. According to one embodiment of the present disclosure, in the step of adding the bacterial liquid, 3.5X10 -3 mg to 5X 10 -2 mg of mycelium is added per cubic centimeter of the culture medium cloth. According to one embodiment of the present disclosure, the specific te