CN-122012244-A - Preparation method of Mortierella alpina preparation

Abstract

The invention relates to a preparation method of a mortierella alpina preparation, which comprises the following steps of fermenting and culturing mortierella alpina inoculation culture medium, centrifuging and taking supernatant, wherein the strain name of mortierella alpina is mortierella alpina (Mortierella alpina) RS-01, and the preservation number is CGMCC No.41944. The mortierella alpina agent provided by the invention can be used for preventing and controlling ginseng rust rot pathogen, melon fusarium wilt pathogen, melon and fruit rot pathogen, apple fruit rot pathogen, corn big spot pathogen, seedling damping-off pathogen, walnut canker pathogen, poplar canker pathogen, pepper anthracnose pathogen, blueberry stem canker pathogen, apple shell monochromatic septoria pathogen and tobacco brown spot pathogen, and has the best antibacterial effect on ginseng rust rot pathogen.

Inventors

• LI LIMEI
• YANG FAN
• MAO HE
• TIAN CHENGLI
• ZHANG XIAOGUANG
• ZHU CHUNYU

Assignees

• 吉林省林业科学研究院(吉林省林业生物防治中心站)

Dates

Publication Date

20260512

Application Date

20250626

Claims (9)

1. 1. A preparation method of a mortierella alpina is characterized by comprising the steps of fermenting and culturing mortierella alpina in an inoculation culture medium, centrifuging and taking supernatant, wherein the strain name of mortierella alpina is mortierella alpina (Mortierella alpina) RS-01, and the preservation number is CGMCC No.41944.
2. 2. The method for preparing the Mortierella alpina preparation according to claim 1, wherein the culture medium is PD liquid culture medium.
3. 3. The method for producing a Mortierella alpina preparation according to claim 1 or 2, wherein the fermentation culture temperature is 28 ℃.
4. 4. The method for producing a Mortierella alpina preparation according to claim 1 or 2, wherein the fermentation culture is conducted at 150 rpm.
5. 5. The method for preparing a Mortierella alpina preparation according to claim 1 or 2, wherein the fermentation culture time is 6 days.
6. 6. The method for producing a Mortierella alpina preparation according to claim 1 or 2, wherein the centrifugal speed is 7800rpm.
7. 7. The method for producing a Mortierella alpina preparation according to claim 1 or 2, wherein the temperature of centrifugation is 25 ℃.
8. 8. The method for preparing a Mortierella alpina preparation according to claim 1 or 2, wherein the centrifugation time is 10 minutes.
9. 9. A biological control method is characterized by comprising the step of adopting a mortierella alpina agent to control plant pathogenic bacteria, wherein the mortierella alpina agent is prepared by adopting the method of any one of claims 1-8, and the plant pathogenic bacteria comprise one or more of ginseng rust rot pathogen, melon and fruit fusarium wilt pathogen, apple fruit rot pathogen, corn northern leaf blight pathogen, seedling damping-off pathogen, walnut canker pathogen, poplar canker pathogen, pepper anthracnose pathogen, blueberry stem canker pathogen, apple shell single color septoria pathogen and tobacco brown spot pathogen.

Description

Preparation method of Mortierella alpina preparation The invention relates to a divisional application, wherein the original Chinese patent application number is 202510872493.6, the application date is 2025, 6 and 26, and the application time is named as Mortierella alpina and application. Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a preparation method of a Mortierella alpina preparation. Background The ginseng, the plant for crude drug of Panax genus of Araliaceae, has high medicinal value. Because of long-term continuous cropping and unreasonable long-time medication, diseases of ginseng are aggravated year by year, and the yield and quality of ginseng are directly affected, wherein the ginseng rust rot can harm each stage of the ginseng growth period, is the most serious root disease of ginseng, has an average incidence rate of 15% -30%, has an incidence rate of more than 70% under serious conditions, and causes serious economic loss. Due to the characteristics of environmental protection and sustainability, the microbial preparation is increasingly widely applied to the aspect of inhibiting the soil-borne diseases of ginseng. In the ginseng production management, microbial agents are adopted to replace chemical fertilizers and pesticides, so that ginseng diseases can be effectively prevented and treated, and the safety of medicinal components of ginseng roots can be ensured. But how to select the appropriate strain is critical to the method. Mortierella (Mortierella) belongs to the class of zygomycetes (Zygomycetes), mucorales (Mucorales), mortierella (Mortierellaceae), and is mainly found in soil, plant residues, animal waste, and the like. Mortierella is also one of the important saprophytic fungi in soil and is widely found in forest soil. Mortierella jenkinii can be used for producing arachidonic acid (ARA), promoting plant growth, repairing pollution, and preparing immunomodulator and antitumor component. However, the report about the prevention and treatment of ginseng rust rot pathogen by mortierella alpina is not yet reported at present. Disclosure of Invention In view of the problems existing in the prior art, the invention provides a preparation method of a mortierella alpina agent. The technical scheme for solving the technical problems is as follows: The invention provides a Mortierella alpina, the strain name is Mortierella alpina (Mortierella alpina) RS-01, the preservation number is CGMCC No.41944, the preservation time is 2025, 05 and 21 days, the Mortierella alpina is preserved in China general microbiological culture Collection center (CGMCC) of China general microbiological culture Collection center, and the preservation place is China institute of microbiological culture Collection, national institute of sciences, national institute 3, no. 1, chengqing, north Star, beijing. The Mortierella alpina RS-01 provided by the invention can be used for preventing and controlling plant pathogenic bacteria, and has the advantages of good prevention and control effect, wide antibacterial spectrum and the like. The invention provides a microbial inoculum, which comprises the Mortierella alpina or the fermentation liquor of the Mortierella alpina. The invention has no special requirement on the dosage form of the microbial inoculum, and can be a solid preparation, a liquid preparation or other preparation types. The microbial inoculum may also include other ingredients, for example, adjuvants, and the like. The microbial agents may also include other microorganisms. The invention provides a fermentation method of Mortierella alpina, which comprises the following steps of fermenting and culturing the Mortierella alpina by using an inoculation culture medium. For example, the fermentation medium may be a PD liquid medium, the culture temperature may be 28℃and the culture time may be 6 days. The fermentation liquor prepared by the method can prevent and treat plant pathogenic bacteria, and has the advantages of good prevention and treatment effect and wide antibacterial spectrum. The invention provides a preparation method of the microbial inoculum, which comprises the following steps of fermenting and culturing the Mortierella alpina by using an inoculation culture medium. For example, the fermentation medium may be a PD liquid medium, the culture temperature may be 28℃and the culture time may be 6 days. Further, the method comprises the step of centrifuging and taking the supernatant. The microbial inoculum prepared by the method can be used for preventing and controlling plant pathogenic bacteria, and has the advantages of good prevention and control effect and wide antibacterial spectrum. The invention provides application of the mortierella alpina or the microbial inoculum in preparation of biocontrol preparations. The invention provides application of the mortierella alpina or the microbial inoculum in preventing and controlling p