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CN-122012248-A - Lentinus edodes strain Le2538 suitable for plate culture and efficient fruiting method thereof

CN122012248ACN 122012248 ACN122012248 ACN 122012248ACN-122012248-A

Abstract

The invention relates to the field of edible fungus strain breeding and cultivation, and provides a mushroom strain Le2538 suitable for flat plate culture, wherein the strain is deposited with the microorganism strain collection in Guangdong province under the accession number GDMCC No:67456, and can stably complete the life history from mycelium to fruiting body on a PDA culture medium. The mode strain Le2538 capable of stably and efficiently completing fruiting body morphogenesis on a PDA flat plate is successfully obtained through directional domestication, the problems that most mushroom strains are blocked in reproduction development under laboratory conditions and cannot form an effective phenotype for quantitative analysis, the matched punching type mycelium stimulation is combined with a low-temperature induction method, stable fruiting can be realized within 30-40 days in a controlled laboratory environment, an experimental system for completely observing the whole process of mushroom mycelium to fruiting body development on the flat plate is successfully established, the whole process only needs conventional laboratory equipment and a cheap PDA culture medium, the operation is simple and convenient, a large amount of manpower and material resources are saved, and the screening flux is greatly improved.

Inventors

  • SONG XIAOXIA
  • SONG CHUNYAN
  • ZHANG MEIYAN
  • SHANG XIAODONG
  • LIU JIANYU

Assignees

  • 上海市农业科学院

Dates

Publication Date
20260512
Application Date
20251219

Claims (9)

  1. 1. A Lentinus edodes strain Le2538 suitable for plate culture is characterized in that the strain is deposited with the microorganism strain collection in Guangdong province under the accession number GDMCC No:67456, and can stably complete the life history from mycelium to fruiting body on a PDA culture medium.
  2. 2. The lentinus edodes strain Le2538 suitable for flat cultivation according to claim 1, wherein the fruiting period of the strain is 30-40 days, the fruiting number of single flat is 2-5, and the structures of the pileus, stipe, shirries, basidiomycetes and basidiomycetes of the fruiting body are complete.
  3. 3. A high efficiency fruiting method suitable for use in Lentinus edodes strain Le2538 according to claim 2, including the steps of: step one, preparing a PDA culture medium; Step two, inoculating and culturing; step three, punching type scratching bacteria; step four, low-temperature induction; and fifthly, fruiting cultivation.
  4. 4. A high efficiency fruiting method of a mushroom strain Le2538 according to claim 3 wherein the PDA culture medium is prepared as follows: Cutting 200g of fresh peeled potatoes without bud eyes into pieces, adding 1000mL of distilled water, boiling for 20-30 min, filtering, adding 20g of glucose and 20g of agar strips into the filtrate, heating and dissolving, adding distilled water to a constant volume of 1000mL, sterilizing for 30min by high-pressure steam at 121 ℃, cooling to 50-60 ℃, pouring into a sterile flat plate, standing for solidification, and adding the agar strips into the filtrate after shearing.
  5. 5. The efficient fruiting method of Lentinus edodes strain Le2538 suitable for plate culture of claim 3 wherein the PDA culture medium in the first step is poured into sterile plates with the diameter of 90mm in an amount of 10-15 mL.
  6. 6. The efficient fruiting method of Lentinus edodes strain Le2538 suitable for flat culture according to claim 3, wherein the inoculation and culture in the second step are as follows: punching holes on a PDA flat plate full of Le2538 mycelium by using a puncher with the diameter of 5mm, inoculating fungus blocks to the center of the PDA flat plate prepared in the first step, performing dark culture at 23-25 ℃ for 7-10 d, sealing the inoculated flat plate by using a sterile sealing film, and keeping the temperature constant in the culture process.
  7. 7. The efficient fruiting method of Lentinus edodes strain Le2538 suitable for flat culture according to claim 3, wherein the perforated mycelium is prepared by selecting 5-30 positions on the surface of a culture flat plate in an ultra-clean workbench by using a sterile puncher with the diameter of 5mm, punching in a uniformly distributed mode, removing part of mycelium blocks, partially preserving in situ, replacing a new sealing film, and culturing in darkness at 23-25 ℃ for 1-3 d.
  8. 8. The efficient fruiting method of Lentinus edodes strain Le2538 suitable for flat-plate culture according to claim 3, wherein the low-temperature induction in the fourth step is to place the flat plate in 10-15 ℃ dark for 2-7 d.
  9. 9. A high efficiency fruiting method of Lentinus edodes strain Le2538 suitable for plate culture according to claim 3 wherein the fruiting culture in the fifth step is as follows: and (3) culturing the flat plate induced by low temperature in darkness at 18-20 ℃ until fruiting bodies are formed, wherein the judging standard of the fruiting body formation is that the structures of the fungus cover, the fungus handle, the fungus pleat, the basidiomycete and the basidiomycete are complete.

Description

Lentinus edodes strain Le2538 suitable for plate culture and efficient fruiting method thereof Technical Field The invention relates to the field of edible fungus strain breeding and cultivation, in particular to a mushroom strain Le2538 suitable for flat plate culture and a high-efficiency fruiting method thereof. Background The commercial cultivation of mushrooms as edible mushrooms with the top global consumption and yield is highly dependent on stable strains with excellent agronomic traits and heterogeneous lignocellulose composite matrix. The cultivation mode is long in period, consumes a large amount of manpower and material resources, and further solves the problems that screening flux is low, phenotypic data signal-to-noise ratio is high, key development events are difficult to observe, terminal agronomic characters are difficult to trace to specific genetic basis and the like due to complex factors such as batch difference of cultivation substrate formulas, variable interference of fruiting environments and the like, so that systematic analysis of mushroom genetic mechanisms and further improvement of breeding efficiency are severely restricted. In basic research, potato dextrose agar medium is a standard substrate for fungus pure culture, physiological and biochemical analysis and strain preservation. However, most of the lentinula edodes cultivated strains show the phenomenon of reproductive retardation on PDA culture medium, the primordium formation of the lentinula edodes cultivated strains has high randomness, the integrity of fruiting body development is difficult to ensure, and the lentinula edodes cultivated strains cannot form an effective phenotype for quantitative analysis. Therefore, the industry and academia have long lacked a set of technical platform capable of rapidly, accurately and repeatedly observing and quantitatively analyzing key characters of the reproductive development of the lentinus edodes in a controlled and standardized laboratory environment. The development of the model mushroom strain which can adapt to the plate culture environment and stabilize fruiting and the matched efficient and standardized fruiting method thereof has vital scientific and industrial significance for accelerating the basic research progress of mushrooms, renovating a genetic breeding system and improving the accuracy of cultivation practice. Disclosure of Invention Aiming at the defects of the prior art, the mode mushroom strain which can adapt to the plate culture environment and stabilize fruiting and the matched high-efficiency standardized fruiting method thereof have important scientific and industrial significance for accelerating the basic research progress of mushrooms, improving the genetic breeding system and improving the accuracy of cultivation practice. The invention is realized by the following technical scheme that the mushroom strain Le2538 suitable for flat-plate culture has the preservation number of GDMCC No:67456, is preserved in the Guangdong province microorganism strain preservation center, and can stably complete the life history from mycelium to fruiting body on a PDA culture medium. Preferably, the fruiting period of the strain is 30-40 days, the fruiting number of single flat plate is 2-5, and the structures of the pileus, the stipe, the fungus pleat, the basidiomycete and the basidiomycete of the fruiting body are complete. An efficient fruiting method suitable for a mushroom strain Le2538, comprising the following steps: step one, preparing a PDA culture medium; Step two, inoculating and culturing; step three, punching type scratching bacteria; step four, low-temperature induction; and fifthly, fruiting cultivation. Preferably, the PDA medium is prepared as follows: Cutting 200g of fresh peeled potatoes without bud eyes into pieces, adding 1000mL of distilled water, boiling for 20-30 min, filtering, adding 20g of glucose and 20g of agar strips into the filtrate, heating and dissolving, adding distilled water to a constant volume of 1000mL, sterilizing for 30min by high-pressure steam at 121 ℃, cooling to 50-60 ℃, pouring into a sterile flat plate, standing for solidification, and adding the agar strips into the filtrate after shearing. Preferably, the PDA culture medium in the first step is poured into a sterile plate with the diameter of 90mm in an amount of 10-15 mL. Preferably, the inoculation and cultivation in the second step are as follows: punching holes on a PDA flat plate full of Le2538 mycelium by using a puncher with the diameter of 5mm, inoculating fungus blocks to the center of the PDA flat plate prepared in the first step, performing dark culture at 23-25 ℃ for 7-10 d, sealing the inoculated flat plate by using a sterile sealing film, and keeping the temperature constant in the culture process. Preferably, in the step three, the perforated mycelium stimulation is performed in an ultra-clean workbench, 5-30 positions are selected on the surface of the