CN-122012249-A - Parasitic wasp friendly multifunctional trichoderma asperellum ZJ12 and application thereof

Abstract

The invention discloses a parasitic wasp friendly multifunctional trichoderma asperellum ZJ12 and application thereof, wherein the preservation number of the bacterial strain is CCTCC NO: M2025805, the bacterial strain has broad-spectrum and high-efficiency antagonistic activity, can effectively inhibit a plurality of plant pathogenic bacteria such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, trichosannthes theaqualis, phytophthora nicotianae, helminthiasis and sphaeria globosa, and the like, has good control effect in potted plant prevention and control experiments, has cross-boundary prevention and control potential on lepidoptera pests (plutella xylostella and cabbage caterpillar), has good biosafety on natural enemy insect parasitic wasps (pteromala pteromannia and parada side-ditch coco), and effectively solves the problem of single function of the existing biocontrol agent.

Inventors

• WANG BEIBEI
• XIE YIXIN
• YUAN QINFENG
• CAO CHUNXIA
• Jin Ruohang
• HUANG DAYE
• ZHENG JIAOLI

Assignees

• 湖北省生物农药工程研究中心

Dates

Publication Date

20260512

Application Date

20260113

Claims (10)

1. 1. A parasitic wasp friendly multifunctional trichoderma asperellum (Trichoderma asperellum) ZJ12 is characterized in that the preservation number is CCTCC NO: M2025805.
2. 2. A microbial agent is characterized in that, comprising the trichoderma asperellum ZJ12 of claim 1.
3. 3. The microbial agent according to claim 2, wherein the active ingredient of the microbial agent is a cell, fermentation broth or conidium of trichoderma asperellum ZJ 12.
4. 4. Use of trichoderma asperellum ZJ12 according to claim 1 or the microbial agent according to claim 2 for controlling plant diseases.
5. 5. The method of claim 4, wherein the plant disease comprises pepper blight, pepper root rot, wheat stem basal rot, cotton verticillium wilt, leaf spot, tobacco black shank, corn leaf spot, and tea leaf black spot.
6. 6. Use of trichoderma asperellum ZJ12 according to claim 1 or a microbial agent according to claim 2 for inhibiting plant pathogens, wherein the pathogens comprise Fusarium oxysporum (Fusarium oxysporum), fusarium solani (Fusarium solani), fusarium pseudograminearum (Fusarium pseudograminearum), verticillium dahliae (Verticillium dahliae), trichoderma tea (Pestalotiopsis theae), phytophthora nicotianae (Phytophthora nicotianae), helminth (Exserohilum turcicum) and cercospora globosa (Nigrospora sphaerica).
7. 7. The method according to claim 4 or 5, wherein the fermentation spore liquid of Trichoderma asperellum ZJ12 is used for soaking plant seeds, or irrigating plant roots, or mixing ZJ12 strain with organic carrier to prepare biological microbial inoculum.
8. 8. Use of trichoderma asperellum ZJ12 according to claim 1 or of a microbial agent according to claim 2 for controlling insect pests.
9. 9. The use according to claim 8, wherein the insect pests include plutella xylostella (Plutella xylostella) and cabbage caterpillar (PIERIS RAPAE).
10. 10. The use according to claim 8 or 9, wherein said trichoderma asperellum ZJ12 or microbial agent is used in combination with parasitic wasps for pest control.

Description

Parasitic wasp friendly multifunctional trichoderma asperellum ZJ12 and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to trichoderma asperellum ZJ12 and application thereof. Background Soil-borne plant diseases caused by fungi and oomycetes are one of the key factors restricting global agricultural production. The pathogens have long survival time and hidden transmission in soil, often cause destructive diseases such as crop wilt, root rot, stem basal rot, yellow wilt and the like, and are extremely difficult to control. Wherein, the verticillium wilt of cotton caused by verticillium dahliae (Verticillium dahliae) and diseases caused by fusarium oxysporum (Fusarium oxysporum), phytophthora spp and the like are widely distributed and diverse in hosts, and form continuous threat to main economic crops such as cotton, capsicum, wheat, tobacco and the like. Meanwhile, lepidoptera pests (such as plutella xylostella Plutella xylostella and cabbage caterpillar PIERIS RAPAE) are another important agricultural pest, and the larvae of the lepidoptera pests directly eat crop leaves, stems and fruits, so that serious yield loss and quality reduction are caused, and the prevention and control pressure is huge. At present, the prevention and treatment of such diseases in agricultural production are still highly dependent on chemical agents. However, the long-term use of chemical pesticides in large quantities is not only easy to cause the pathogenic bacteria to generate drug resistance, but also can cause the problems of pesticide residues, soil microecological imbalance, environmental pollution and the like. With the increasing importance of global quality safety and ecological environment protection of agricultural products, development of efficient, green and sustainable disease prevention and control strategies has become an urgent need in the agricultural field. The control of plant diseases by beneficial microorganisms is an important development direction for green agriculture. The trichoderma fungi are used as important biocontrol microorganisms, and can realize the comprehensive control of plant fungal diseases through multiple mechanisms. In the aspect of disease control, trichoderma effectively inhibits infection and expansion of pathogenic bacteria by competing for niches, re-hosting pathogenic bacteria (secreting hydrolytic enzymes such as chitinase and glucanase to degrade the cell walls of the pathogenic bacteria), generating antibacterial secondary metabolites, inducing plant system resistance and the like. On the basis, the invention further focuses on the potential pest control value of the trichoderma fungi beyond the traditional disease prevention and control function. Different from the development thought of the existing microbial pesticide single target, the invention discovers that the feeding behavior and survival of lepidoptera pest larvae can be directly influenced by the activity or parasitic characteristic of the metabolite of the specific trichoderma strain, thereby realizing the integrated biological prevention and control of diseases and insect pests. Furthermore, currently most microbial pesticides are registered and safety rated mainly for their efficacy against target organisms (pathogenic bacteria or pests), and there is a general lack of systematic safety assessment against non-target organisms, in particular natural enemy insects (such as parasitic bees, which play a key role in ecological regulation). The existing trichoderma preparation still faces the challenges of single function, unstable prevention effect, unknown potential influence on non-target organisms and the like in practical application, and particularly has the advantages of efficient bacteriostasis, remarkable disinsection, ecological safety and compatibility with natural enemies, and the multifunctional strain resource is still scarce, so that the trichoderma preparation is restricted from being further popularized and applied. Therefore, the separation and screening of the trichoderma strains with high efficiency, bacteriostasis, remarkable disinsection, safety to natural enemies and other functions have become a key task for developing biological pesticides and promoting green transformation of agriculture. By breeding excellent strains with integrated functions and ecological friendliness, the bottleneck of single functions of the traditional microbial inoculum can be effectively overcome, the health level of crops can be improved, the dependence on chemical pesticides can be reduced through a comprehensive prevention and control mode of 'one bacterium multiple-effect' and cooperation with natural enemies, and key resources and technical support are provided for constructing a stable and sustainable plant protection system. Disclosure of Invention Aiming at the problems, the invention provides trichoderma asperellum ZJ12, a microbial ag