CN-122012250-A - Poria cocos strain with high disease resistance and high yield and application thereof

Abstract

The invention discloses a tuckahoe fungus (Wolfiporia cocos) Wc2408, which has a preservation number of CCTCC NO: M2026123 in China center for type culture collection, and can still keep normal growth and cover mould colony under the condition that soil competitive fungi represented by trichoderma longibrachiatum exist in a plate counter experiment; compared with commercial strains, the strain has the capability of resisting the infection of hybrid bacteria such as trichoderma longibrachiatum and the like, has high tuckahoe forming rate and single plant yield, provides a strain with strong space competitiveness, stress resistance and high yield of tuckahoe for the production of tuckahoe, and is suitable for large-scale production and market popularization.

Inventors

• YAN JINPING
• LIN JINGXI
• WU QIAN
• YANG EN

Assignees

• 昆明理工大学

Dates

Publication Date

20260512

Application Date

20260204

Claims (4)

1. 1. A Poria (Wolfiporia cocos) Wc2408 has a preservation number of CCTCC NO: M2026123 in China center for type culture collection.
2. 2. The fungus Wc2408 of claim 1, which is characterized by having a soil-competing fungal activity represented by Trichoderma longibrachiatum (Trichoderma longibrachiatum), trichoderma harzianum (T. Harzianum), and Trichoderma viride (T. Viride).
3. 3. Use of the pachymella Wc2408 according to claim 1 or 2 for cultivating and increasing yield of pachyma cocos.
4. 4. The application of the culture medium according to claim 3, wherein the culture medium formula of the poria cocos cultivation fungus bag comprises 50-65% of pine wood dust, 15-25% of miscellaneous wood dust, 8-12% of bran, 8-12% of corn flour, 0.5-1.5% of sucrose, 0.5-1.5% of gypsum, 0.2-0.6% of magnesium sulfate and the water content of the culture medium is 55% -60%.

Description

Poria cocos strain with high disease resistance and high yield and application thereof Technical Field The invention belongs to the technical field of edible and medicinal fungus strain resources and cultivation, and particularly relates to a strain of tuckahoe fungus (Wolfiporia cocos) Wc2408 and application thereof in tuckahoe cultivation for resisting soil competitive fungi and improving tuckahoe yield, wherein the strain has the soil competitive fungus activity represented by trichoderma longibrachiatum (Trichoderma longibrachiatum), trichoderma harzianum (T. Harzianum) and trichoderma viride (T. Viride) inhibition. Background Poria [ Wolfiporia cocos (Schwein.) RYVARDEN ET Gilb ] belongs to Polyporaceae, poria, and is a traditional fungus with homology of medicine and food in China. The dry sclerotium is used as a medicine, and has the effects of promoting diuresis, removing dampness, strengthening spleen, soothing nerves and the like. Modern pharmacological researches show that poria cocos is rich in various bioactive components such as triterpenes and polysaccharides, and has remarkable application value in aspects of immunoregulation and the like. Along with the increase of market demands and the difficulty in obtaining wild resources of tuckahoe sclerotium, the development of manual standardized cultivation of tuckahoe has important significance for guaranteeing the stable supply of raw materials. At present, the artificial cultivation of poria cocos mainly takes pine as a matrix and is carried out in an open environment. The mode simulates natural habitat to a certain extent, but because of a large amount of competitive mixed bacteria in culture medium and environment, and loss of natural antibacterial components of pine in the air drying process, the pine is extremely easy to be infected by the mixed bacteria in a longer growth period, so that bacterial decay, tuckahoe mycelium colonization inhibition, wood conversion reduction, sclerotium yield reduction, mildew and the like are caused. The saprophytic fungi represented by Trichoderma, penicillium and the like exist in a large amount in soil, and proper conditions can cause fungus bags to mildew in edible fungus cultivation, competitively inhibit the diseases such as edible fungus mycelium colonization, primordium and mature fruiting body mildew and the like, and are commonly called as 'green mold'. Common infection of trichoderma in soil such as trichoderma harzianum (t. Harzianum), trichoderma longibrachiatum (t. Longibrachiatum) and the like can inhibit the growth of target hyphae, resulting in reduced yield and even failure of cultivation. The existing control measures mainly depend on chemical agents or environmental regulation, but chemical control has the risk of pesticide residues, and the breeding of good varieties with high yield and broad-spectrum resistance also has technical bottlenecks. Therefore, the poria cocos germplasm resources with natural disease resistance are obtained through screening, and the method has important practical significance for improving the biological safety and yield stability of poria cocos cultivation. Disclosure of Invention The invention provides a tuckahoe fungus (Wolfiporia cocos) Wc2408 with strong space competition capability, stress resistance and high yield tuckahoe, which is preserved in China Center for Type Culture Collection (CCTCC) No. M2026123 at the 1 st month 15 days 2026 and has a preservation address of university of Wuhan in China. The tuckahoe bacteria Wc2408 of the invention is a tuckahoe strain with capability of resisting infectious microbe infection, and soil competitive fungi represented by Trichoderma (Trichoderma spp.) causing tuckahoe 'green mold' include, but are not limited to, trichoderma longibrachiatum (Trichoderma longibrachiatum), trichoderma harzianum (t. Harzianum), trichoderma viride (t.ride). Despite the differences in molecular characteristics of different species of trichoderma, the mechanism by which Poria cocos is infected is substantially similar, relying on rapid growth and secretion of cell wall degrading enzymes to preempt the niche. The poria cocos strain Wc2408 can effectively inhibit the spread of pathogenic bacteria, partially and even completely cover the colony of the trichoderma longibrachiatum and reduce the spore yield of the trichoderma longibrachiatum by forming a compact antagonistic band when facing to the high-speed growth pressure of the trichoderma longibrachiatum, thereby representing a backward extrusion effect on the growth space of the trichoderma longibrachiatum. Another object of the present invention is to apply the above Poria cocos Wc2408 in Poria cocos cultivation and improving Poria cocos yield; The cultivation mode comprises bag material cultivation or log cultivation, wherein the log is preferably pine wood, and the cultivation environment can be greenhouse cultivation, under-forest cultivation, wild-like cultivation or factory cu