CN-122012253-A - Penicillium ochloricum Ynau-PO resistant to lead and cadmium and antagonistic to root rot of pseudo-ginseng and application thereof

Abstract

The invention provides penicillium ochraceum Ynau-PO which is resistant to lead and cadmium and antagonizes pseudo-ginseng root rot fungi and application thereof, and relates to the field of microorganisms. The penicillium ochloricum Ynau-PO has a preservation number of CCTCC NO: M20252802, can simultaneously have high-efficiency lead and cadmium resistance and pseudo-ginseng root rot resistance, solves the technical bottlenecks of low microorganism survival rate and poor restoration effect in high-concentration lead and cadmium polluted soil, and provides an environment-friendly biological control scheme to reduce the use of chemical pesticides and avoid the generation of environmental pollution and pathogenic bacteria drug resistance.

Inventors

• MEI XINYUE
• Huang huichuan
• ZHU SHUSHENG
• KONG LINGJUAN
• YANG YAN
• LIU YINGPIN
• DENG WEIPING
• YANG MIN
• DU FEI
• YE CHEN
• LIU YIXIANG

Assignees

• 云南农业大学

Dates

Publication Date

20260512

Application Date

20260226

Claims (5)

1. 1. Penicillium ochloricum (Penicillium ochrochloron) Ynau-PO resistant to lead and cadmium and antagonistic to pseudo-ginseng root rot fungi is characterized in that the preservation number of the penicillium ochloricum Ynau-PO is CCTCC NO: M20252802.
2. 2. Use of penicillium ochloricum Ynau-PO according to claim 1 for remediation of lead and/or cadmium contaminated environments.
3. 3. The method according to claim 2, wherein the Penicillium ochloricum Ynau-PO has a lead resistance up to 2000mg/L and a cadmium resistance up to 500mg/L in the treatment of lead and/or cadmium contaminated environments.
4. 4. Use of penicillium ochloricum Ynau-PO according to claim 1 for biological control of root rot of notoginseng.
5. 5. The method of claim 4, wherein the pathogenic fungus of Panax notoginseng root rot is at least one of Fusarium oxysporum (Fusarium oxysporum), fusarium solani (Fusarium solani), leuconostoc mesenteroides (Cylindrocarpon destructans), and Phytophthora infestans (Phytophthora cactorum).

Description

Penicillium ochloricum Ynau-PO resistant to lead and cadmium and antagonistic to root rot of pseudo-ginseng and application thereof Technical Field The invention relates to the field of microorganisms, in particular to penicillium ochrome Ynau-PO which is resistant to lead and cadmium and antagonizes root rot of pseudo-ginseng and application thereof. Background Soil heavy metal pollution and plant soil-borne diseases are two major problems restricting agricultural production and ecological environment. In the current repair methods, bioremediation, particularly microbial repair, is of great interest because of its environmental friendliness and low cost. Some fungi (such as Aspergillus and Penicillium) have heavy metal resistance and other fungi (such as Trichoderma) have biocontrol function, namely the existing strains generally only have one of heavy metal resistance and biocontrol function, and cannot realize the synergy of 'repair' and 'disease prevention', and the strains special for biocontrol are difficult to survive in heavy metal contaminated soil and cannot play a role. The disclosed penicillium still has a certain limit on the concentration of heavy metals, and the penicillium strain which has high-efficiency lead/cadmium resistance and the capacity of antagonizing the root rot of pseudo-ginseng at the same time is not reported at present. Disclosure of Invention Aiming at the defects of the prior art, the invention provides penicillium ochraceum Ynau-PO which is resistant to lead and cadmium and antagonizes pseudo-ginseng root rot and application thereof, and the strain has the capabilities of high-efficiency lead/cadmium resistance and pseudo-ginseng root rot antagonism. In order to achieve the above purpose, the invention is realized by the following technical scheme: The penicillium ochloricum Ynau-PO which is resistant to lead and cadmium and antagonizes pseudo-ginseng root rot fungi is characterized in that the preservation number of the penicillium ochloricum Ynau-PO is CCTCC NO: M20252802. The penicillium ochloricum Ynau-PO is applied to the environment for treating the lead and/or cadmium pollution, and the concentration of lead resistance of the penicillium ochloricum Ynau-PO reaches 2000mg/L and the concentration of cadmium resistance reaches 500mg/L when the environment for treating the lead and/or cadmium pollution is treated. The penicillium ochloricum Ynau-PO is applied to biological control of the root rot of pseudo-ginseng, and pathogenic fungi of the root rot of pseudo-ginseng are at least one of fusarium oxysporum, fusarium solani, post-infection and phytophthora capsici. The invention provides penicillium ochrome Ynau-PO which is resistant to lead and cadmium and antagonizes pseudo-ginseng root rot fungi and application thereof, and has the advantages compared with the prior art that: The invention solves the problem of single function of the strain in the existing microorganism repairing technology, provides a difunctional microorganism which can not only efficiently resist heavy metal lead/cadmium but also effectively antagonize pseudo-ginseng root rot, solves the technical bottlenecks of low microorganism survival rate and poor repairing effect in high-concentration lead-polluted soil, and provides an environment-friendly biological control scheme to reduce the use of chemical pesticides and avoid the generation of environmental pollution and pathogenic bacteria drug resistance. Drawings FIG. 1 is a schematic diagram showing the growth of the front and back sides of Penicillium ochloricum Ynau-PO under different lead concentrations; FIG. 2 is a schematic diagram showing the inhibition rate of Penicillium ochloricum Ynau-PO under different lead stress concentrations according to the present invention; FIG. 3 shows the front and back growth of Penicillium ochloricum Ynau-PO under different concentrations of cadmium stress according to the present invention; FIG. 4 is a schematic diagram showing the inhibition rate of Penicillium ochloricum Ynau-PO under different concentrations of cadmium stress according to the present invention; FIG. 5 is a schematic diagram showing antagonism of Penicillium ochloricum Ynau-PO to different root rot pathogens according to the present invention, wherein the upper part is the growth of the root rot pathogens and the lower part is the growth of the root rot pathogens after inoculation with Penicillium ochloricum Ynau-PO; FIG. 6 shows the inhibition rate of Penicillium ochloricum Ynau-PO of the present invention against different root rot pathogens; fig. 7 is a graph showing the incidence of notoginseng in different treatment groups according to the present invention. Detailed Description For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described in the following i