CN-122012254-A - Acidovorax carnosus mutant strain for producing extracellular green pigment and fermentation method and application thereof

Abstract

The invention discloses a mutant strain of cheilis carnosus for producing extracellular green pigment, and a fermentation method and application thereof, and belongs to the technical field of microorganisms. The technical problem to be solved is to provide the meat color Ji Si fungus which can exocrine green pigment, has high yield and good stability, and the green pigment obtained by fermentation of the meat color Ji Si fungus has stable property. The key point of the technical scheme is that the Botrytis cinerea (Keithomyces carneus) BG-G1-uv03 is deposited in the Guangdong province microorganism strain collection center, and the deposit number is GDMCC NO:67281. The extracellular green pigment-producing flesh fungus mutant strain and the optimized fermentation process provided by the invention provide new strain resources and technical schemes for the industrial production of natural microbial pigments, and have important economic value and environmental significance.

Inventors

• ZHU XINYAN
• FANG HAOMING
• YE TING
• CHEN SHILEI

Assignees

• 荟来生物科技(深圳)有限公司

Dates

Publication Date

20260512

Application Date

20260302

Claims (10)

1. 1. A strain of Botrytis cinerea (Keithomyces carneus) BG-G1-uv03 is characterized in that the Botrytis cinerea BG-G1-uv03 is deposited in the Guangdong province microorganism strain collection, and the deposit number is GDMCC NO:67281.
2. 2. An original strain for preparing the bordetella moreus BG-G1-uv03 of claim 1, wherein the original strain is bordetella moreus (Keithomyces carneus) BG-G1 deposited with the cantonese microbiological strain deposit center under the deposit number GDMCC NO:67280.
3. 3. The preparation method of the bordetella moreus BG-G1-uv03 of claim 1, which is characterized in that the preparation method comprises the steps of screening and purifying the bordetella moreus BG-G1 of claim 2 after being irradiated by ultraviolet light.
4. 4. Use of bordetella carnosa BG-G1-uv03 according to claim 1 for the fermentative production of green pigment.
5. 5. A preparation method of a green pigment fermentation broth, which is characterized in that the preparation method is to inoculate the bordetella moreus BG-G1-uv03 in the culture broth for fermentation.
6. 6. The preparation method according to claim 5, characterized in that the preparation method comprises the steps of: S1, inoculating the meat color basic Si fungus BG-G1-uv03 in the claim 1 into a culture medium to obtain an activated strain; S2, inoculating the activated strain into a seed culture medium for culture to obtain a liquid strain; S3, inoculating the liquid strain into a fermentation medium, and fermenting by adopting a fermentation tank feed supplementing fermentation process to obtain a green pigment fermentation liquor; The fermentation tank feed supplementing fermentation process in the step S3 comprises a thallus propagation stage and a feed supplementing stage.
7. 7. A green pigment fermentation broth prepared by the method of any of claims 5-6.
8. 8. A method for promoting the dissolution of the cytochrome BG-G1-uv03 of bouillon-base fungus according to claim 1, comprising adding a complex biological enzyme agent and ethyl salicylate to the green pigment fermentation broth of claim 7.
9. 9. A method for preparing a green dye solution by extracting green pigment from the green pigment fermentation liquor according to claim 7, which is characterized by comprising the following steps of taking the green pigment fermentation liquor, centrifuging, taking supernatant, adding a calcium chloride solution, uniformly mixing, standing to obtain a yellowish green turbid solution, centrifuging, discarding the supernatant to obtain a green precipitate, air-drying to obtain the purified green pigment, and adding a dilute hydrochloric acid solution.
10. 10. Use of the green dye liquor prepared by the method of claim 9 in food processing, cosmetic preparation, textile printing and dyeing.

Description

Acidovorax carnosus mutant strain for producing extracellular green pigment and fermentation method and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a Bursaphenous-meat mutant strain for producing extracellular green pigment, a fermentation method and application thereof. Background Pigments are widely used as important colorants in various industries such as food processing, cosmetic preparation, textile printing and dyeing. Although the traditional synthetic pigment has the characteristics of strong tinting strength, low cost and the like, the problems of environmental residual pollution and human health risks caused by the traditional synthetic pigment are increasingly prominent in recent years, and under the background, the development of natural, safe and biodegradable natural pigment to replace the synthetic pigment has become an important development trend in the fields of food industry, fine chemical industry and the like. The microbial pigment has the advantages of ecological environmental protection, wide raw material sources, short fermentation period, large-scale production and the like in the production process, is considered as a sustainable substitute with the most potential of synthesizing the pigment, and has wide application prospect in the fields of foods, cosmetics, textiles, medicines and the like. Wherein, fungi are taken as one of important sources of natural pigments, the produced pigments have rich components, and the fungi have proved to contain various compounds such as phenols, terpenes, quinones, quinolines and the like, and part of fungi pigments also have excellent biological activities such as antioxidation, anti-aging, antibiosis and the like, thereby further improving the application value. Fungi can synthesize various pigments, including secondary metabolites with complex structures such as melanin, carotenoid, flavonoid pigment and the like, but compared with pigments with other colors, fungi resources for producing green pigment are less common. Prior studies have shown that the green appearance of fungi is often associated with the color of conidia (asexual spores) or mycelium, a characteristic of a number of important fungal genera, such as Metarhizium (Metarhizium), trichoderma (Trichoderma), penicillium (Penicillium), and the like. However, most of the green pigment produced by the fungi is intracellular pigment, and the problems of complex extraction process, low extraction rate and high production cost exist, so that the industrialized application of the green pigment is limited. The related patent related to fungus green pigment has the technical proposal mainly focused on the application research of extracting the green pigment from fungus green spores or mycelium, and has not been reported in the related report of producing the green pigment by fermenting the meat color Ji Si fungus (Keithomyces carneus). In the prior research paper, the research on the sarcoidosis is limited to culturing the bacteria by adopting a basic culture medium, and morphological observation and taxonomic identification are further carried out, and related research on pigment production of the bacteria is not involved, and how to realize the extracellular secretion and yield improvement of the pigment is not mentioned. Meanwhile, the existing fungal pigment has two general technical bottlenecks, namely that part of fungal pigment is sensitive to environmental factors such as light, heat and the like, is easy to degrade in the processing, storage and application processes, so that the coloring effect is unstable, the application range is greatly limited, and the pigment yield of wild strains is generally low, so that the requirement of industrial large-scale production is difficult to meet. Therefore, screening fungus strains with high pigment yield, good environmental stability and exocrine ability and optimizing the fermentation process thereof becomes a technical problem to be solved in the current research field of natural fungus pigments. The related patent documents: Publication No. CN112778797B, publication No. 2021.05.11, which discloses a method for extracting natural green pigment from Metarrhizium anisopliae, belongs to the technical field of biological product extraction and preparation. The operation steps comprise culturing strain with fungus of Metarrhizium, culturing to collect spores, removing impurities from spores, preparing pigment crude extract, concentrating, drying, refining pigment precipitate, and drying to obtain dark green powdered natural green pigment. The relevant non-patent literature: Journal name "plant protection", literature name "biological Activity of Metarhizium and its close genus fungi from the Dacheng's litter", volume No. 2021,47 (05), publication date 2021.10.12, which is a study of the biological activities of Metarhizium and its close genus fungi, identified Meta