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CN-122012255-A - Yellow Lan Zhuang strain and application thereof

CN122012255ACN 122012255 ACN122012255 ACN 122012255ACN-122012255-A

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a strain of Lan Zhuang bacteria and application thereof. The invention discloses a strain of yellow Lan Zhuang bacteria H12 with a preservation number of CGMCC No.42336. The yellow blue fungus H12 is separated and screened from the natural world for the first time, and can generate remarkable antagonism on a pathogen causing the root rot of fritillaria taipaiensis, namely a fusarium oxysporum strain. Through identification, the fermentation liquor and volatile substances of the flavobacterium H12 can destroy pathogenic hypha structures, so that hypha deformity is distorted, and the inhibition rates of the hypha growth reach 58.54% and 28.02% respectively. The results of potted plants and fields show that the application of the H12 fermentation liquor can obviously reduce the incidence rate and the disease index of the fritillaria taipaiensis root rot, and the final control effect reaches 56.16 percent, which is equivalent to chemical pesticides.

Inventors

  • WANG JIPENG
  • LI YONG
  • SUN NIANXI
  • PENG RUI
  • YANG XUE
  • HAN XUE
  • Duan Yanxue
  • MA CHAO

Assignees

  • 重庆市中药研究院
  • 西南大学

Dates

Publication Date
20260512
Application Date
20260320

Claims (10)

  1. 1. A strain of yellow Lan Zhuang bacteria (Talaromyces flavus) H12 is characterized in that the preservation number is CGMCC No.42336.
  2. 2. A culture of the microorganism H12 of claim 1.
  3. 3. The culture of claim 2, wherein the culture of cyanobacteria flavum H12 comprises Lan Zhuang bacteria H12 and secondary metabolites of cyanobacteria flavum H12; The secondary metabolite comprises volatile substances, wherein the volatile substances comprise one or more than two of 4-lactone, hexaketone, 5-hydroxymethylfurfural and 4-carboxyl-5-hydroxyphthalimide methyl ester.
  4. 4. A microbial agent, wherein an active ingredient of the microbial agent comprises the cyanobacteria flavescens H12 of claim 1 or the culture of claim 2 or 3.
  5. 5. The microbial agent according to claim 4, wherein, the effective viable count of the yellow blue fungus H12 in the microbial agent is more than or equal to 2 multiplied by 10 9 CFU/ml.
  6. 6. Use of the xanthomonas H12 of claim 1, the culture of claim 2 or 3 or the microbial agent of claim 4 or 5 for controlling plant root rot.
  7. 7. The use according to claim 6, wherein the plant comprises fritillaria taipaiensis; The root rot includes a root rot caused by fusarium oxysporum.
  8. 8. A method for controlling plant root rot, comprising the steps of: Application to fritillaria taipaiensis root soil using the cyanobacteria H12 of claim 1, the culture of claim 2 or 3, or the microbial inoculum of claim 4 or 5.
  9. 9. The method of claim 8, wherein the means of application comprises irrigation with water; The irrigation dose of the culture or microbial agent is 5ml/m 2 ~15ml/m 2 ; The culture or microbial agent is irrigated once every 7d for 3 times in total; The effective viable count in the culture or microbial agent is more than or equal to 2 multiplied by 10 9 CFU/ml.
  10. 10. The method of claim 8, wherein the plant comprises fritillaria taipaiensis; The root rot includes a root rot caused by fusarium oxysporum.

Description

Yellow Lan Zhuang strain and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a strain of Lan Zhuang bacteria and application thereof. Background The fritillaria taipaiensis is perennial herb of fritillaria genus of Liliaceae family, and the dry bulb is one of the Bulbus Fritillariae Cirrhosae base sources collected in Chinese pharmacopoeia of calendar, has effects of moistening lung for arresting cough, resolving phlegm, relieving asthma, resolving hard mass, detumescence, etc., and is clinically used for lung heat dry cough, lung yin deficiency, etc. Because wild resources are becoming deficient due to excessive digging and environmental damage, fritillaria taipaiensis is already listed in the national important protection of wild plants directory, and artificial cultivation becomes an effective way for protecting and utilizing the rare species. However, with the expansion of cultivation area, soil-borne diseases, especially root rot, have become a major factor restricting the safe production of fritillaria taipaiensis. Root rot mainly damages the root and bulb of fritillaria taipaiensis, has extremely high incidence rate in low-lying ponding areas, can be transmitted by soil nematodes and moisture, can cause bulb rot even absolute production when serious, and forms a remarkable threat to yield and quality. At present, chemical pesticides are still used as main pesticides for preventing and treating the disease, but the problems of short lasting period and unstable field prevention effect exist. Disclosure of Invention The invention aims to solve the problems in the prior art and find a green, safe, efficient and stable scheme for preventing and treating fritillaria taibaiensis root rot. Therefore, the invention provides a strain of Lan Zhuang yellow bacteria and application thereof. The invention provides a strain of Lan Zhuang yellow fungus (Talaromyces flavus) H12 with a preservation number of CGMCC No.42336. The invention also provides a culture of the cyanobacteria flavescens H12. Preferably, the culture of the cyanobacterium flavum H12 comprises the secondary metabolites of the cyanobacterium flavum H12 and the cyanobacterium flavum H12; The secondary metabolite comprises volatile substances, wherein the volatile substances comprise one or more than two of 4-lactone, hexaketone, 5-hydroxymethylfurfural and 4-carboxyl-5-hydroxyphthalimide methyl ester. The invention also provides a microbial agent, and the effective components of the microbial agent comprise the yellow blue fungus H12 or the culture according to the technical scheme. Preferably, the effective viable count of the ceruloplasmia flavescens H12 in the microbial agent is more than or equal to 2 multiplied by 10 9 CFU/ml. The invention also provides application of the flavobacterium H12 in the technical scheme, the culture in the technical scheme or the microbial agent in the technical scheme in preventing and treating plant root rot. Preferably, the plant comprises fritillaria taipaiensis; The root rot includes a root rot caused by fusarium oxysporum. The invention also provides a method for preventing and treating plant root rot, which comprises the following steps: the blue-yellow fungus H12, the culture or the microbial agent according to the technical scheme is applied to the fritillaria taipaiensis root soil. Preferably, the means of application comprises irrigation with water; The irrigation dose of the culture or microbial agent is 5ml/m 2~15ml/m2; The culture or microbial agent is irrigated once every 7d for 3 times in total; the effective viable count in the culture or microbial agent is more than or equal to 2 multiplied by 10 9 CFU/ml. Preferably, the plant comprises fritillaria taipaiensis; The root rot includes a root rot caused by fusarium oxysporum. The beneficial effects are that: the invention provides a strain of yellow Lan Zhuang bacteria H12 with a preservation number of CGMCC No.42336. The yellow blue fungus H12 is obtained by separating and screening from the natural world for the first time, can generate remarkable antagonism on a pathogen of fritillaria taipaiensis root rot, namely fusarium oxysporum strain, and the fermentation liquor and volatile substances (such as 4-lactone, hexaketone, 5-hydroxymethylfurfural, 4-carboxyl-5-hydroxyphthalimide methyl ester and the like) of the yellow blue fungus H12 can destroy pathogenic mycelium structures, so that mycelium deformity is caused, and the inhibition rate on mycelium growth is up to 58.54% and 28.02% respectively. The results of potted plants and fields show that the application of the H12 fermentation liquor can obviously reduce the incidence rate and the disease index of the fritillaria taipaiensis root rot, and the final control effect reaches 56.16 percent, which is equivalent to chemical pesticides. In addition, in the field test, the H12 fermentation liquid also obviously improves