CN-122012263-A - Isofloxacin Botrytis cinerea with potassium super-enrichment capability and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to Isofaciens Botrytis cinerea with potassium super-enrichment capability and application thereof. Aiming at the problem of lacking a strain capable of enriching potassium ions in wastewater in the prior art, the Isophtalmic Botrytis cinerea (Blastobotrys allociferrii) JK-1 is preserved in China general microbiological culture Collection center (CGMCC) No.39121 in the year of 2026, 01 and 12. The strain JK-1 is the first discovered Isofaciens Botrytis cinerea with the characteristic of potassium super enrichment, and can not only purify biogas slurry, but also be used as a biological pump for concentrating and recycling potassium resources.

Inventors

• LI DONG
• JIA FENGLIN

Assignees

• 中国科学院成都生物研究所

Dates

Publication Date

20260512

Application Date

20260210

Claims (6)

1. 1. The Botrytis cinerea (Blastobotrys allociferrii) JK-1 is characterized in that the Botrytis cinerea JK-1 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 39121 in 2026, 01 and 12 days.
2. 2. A bacterial preparation produced using the Botrytis cinerea JK-1 of claim 1, or a bacterial preparation comprising the Botrytis cinerea JK-1 of claim 1.
3. 3. Use of the Botrytis cinerea JK-1 according to claim 1 or the bacterial preparation according to claim 2 in the treatment of potassium-containing wastewater.
4. 4. The method according to claim 3, wherein the wastewater is treated to reduce the concentration of ammonia nitrogen in the wastewater containing potassium and/or to reduce the concentration of potassium ions in the wastewater containing potassium.
5. 5. The application of the Botrytis cinerea JK-1 or the bacterial preparation in the enrichment of potassium ions as claimed in claim 1 is characterized in that the Botrytis cinerea JK-1 or the bacterial preparation is inoculated into an environment to be enriched with potassium ions, and the temperature of the environment is controlled to be 20-33 ℃.
6. 6. Use of a rhodosporidium isofram JK-1 according to claim 1 or a bacterial preparation according to claim 2 for transferring potassium ions, characterized in that the use comprises the steps of: (1) Inoculating the Botrytis cinerea JK-1 or the bacterial preparation into an environment rich in potassium ions to enrich the potassium ions, and controlling the environment temperature to be 20-33 ℃; (2) And inoculating the Botrytis cinerea JK-1 or the bacterial preparation enriched with potassium ions into an environment in need of increasing the concentration of the potassium ions, and controlling the temperature of the environment to be 0-6 ℃.

Description

Isofloxacin Botrytis cinerea with potassium super-enrichment capability and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to Isofaciens Botrytis cinerea with potassium super-enrichment capability and application thereof. Background At present, a method for effectively recycling potassium ions in wastewater at low cost is lacking. The production of Single Cell Protein (SCP) by converting wastewater with microorganisms is an effective way to realize the resource utilization of wastewater (such as biogas slurry). However, existing yeast strains (such as Saccharomyces cerevisiae, candida utilis and the like) for wastewater treatment often have the defects of insufficient tolerance to high-concentration ammonia nitrogen and complex inhibitors in wastewater such as biogas slurry, slow growth, high salt risk of the treated biogas slurry due to lack of an efficient enrichment mechanism for specific mineral potassium ions, difficulty in low-cost separation of potassium ions enriched in cells, excessively high SCP ash content produced and influence on feed quality. Therefore, a new strain with the characteristic of 'super potassium absorption' is screened, and the method has important industrial application value. Disclosure of Invention The invention aims to provide a Botrytis cinerea strain with potassium super-enrichment capability and application thereof. In order to achieve the aim of the invention, the technical scheme adopted by the invention is that the Botrytis cinerea (Blastobotrys allociferrii) JK-1 is preserved in China general microbiological culture collection center (CGMCC) No.39121 in 2026, 01 and 12 days. Correspondingly, the bacterial preparation prepared by using the Botrytis cinerea strain JK-1 or the bacterial preparation containing the Botrytis cinerea strain JK-1. Correspondingly, the Botrytis cinerea JK-1 or the application of the bacterial preparation in potassium-containing wastewater treatment. The wastewater treatment is to reduce the ammonia nitrogen concentration in the wastewater and/or reduce the potassium ion concentration in the potassium-containing wastewater. Correspondingly, the application of the Botrytis cinerea JK-1 or the bacterial preparation in enriching potassium ions is that the Botrytis cinerea JK-1 or the bacterial preparation is inoculated into an environment to be enriched with potassium ions, and the environmental temperature is controlled to be 20-33 ℃. Correspondingly, the application of the Botrytis cinerea JK-1 or the bacterial preparation in transferring potassium ions comprises the following steps: (1) Inoculating the Botrytis cinerea JK-1 or the bacterial preparation into an environment to be enriched with potassium ions to enrich potassium ions, and controlling the environmental temperature to be 20-33 ℃; (2) And inoculating the Botrytis cinerea JK-1 or the bacterial preparation enriched with potassium ions into an environment in need of increasing the concentration of the potassium ions, and controlling the temperature of the environment to be 0-6 ℃. The strain provided by the invention is the first discovered Isofaciens Botrytis cinerea with the characteristic of potassium super enrichment, and can not only purify biogas slurry, but also be used as a biological pump for concentrating and recycling potassium resources. The strain is used for treating potassium-containing wastewater such as biogas slurry, the potassium ion removal rate is up to more than 96%, the ammonia nitrogen removal rate is up to 96%, and the pollution load of the wastewater can be obviously reduced. The strain JK-1 provided by the invention can grow rapidly in high-concentration biogas slurry (ammonia nitrogen concentration is more than 3000 mg/L) by taking the biogas slurry as a sole nitrogen source/main nutrition source. Under the normal-temperature aerobic condition, the strain JK-1 has extremely strong capacity of actively transporting potassium ions and stronger ammonia nitrogen digestion capacity. Drawings FIG. 1 is a colony morphology of strain JK-1; FIG. 2 is an optical microscopy image of strain JK-1; FIG. 3 is a schematic diagram showing the growth curve of strain JK-1; FIG. 4 is a schematic diagram of the overlapping region of the molecular characterization sequence of strain JK-1. Detailed Description The invention provides a strain of Issatchiff's Botrytis cinerea (Blastobotrys allociferrii) JK-1 with the characteristic of potassium super enrichment, which is preserved in China general microbiological culture Collection center (CGMCC) in the year 2026, 01 and 12, wherein the preservation address is CGMCC No.39121 of China academy of sciences of China, the West-Lou one number one, and the preservation number is North Star in the Beijing city. The characteristic sequence of the strain JK-1 is shown as SEQ ID NO. 1, 1-1795 bp is an 18S-SSU sequence, 1744-2354 bp is an ITS sequence, 2335-2954 bp is a