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CN-122012266-A - Nitrogen-phosphorus-potassium composite microbial inoculant, and preparation method and application thereof

CN122012266ACN 122012266 ACN122012266 ACN 122012266ACN-122012266-A

Abstract

The invention relates to a nitrogen-phosphorus-potassium composite microbial inoculum, a preparation method and application thereof, wherein a bacterial strain of the composite microbial inoculum consists of Pseudomonas sp 1416X3, proprietaria sp Priestia sp ST2 and Burkholderia sp YHN 3-13. The invention compounds three specific strains with different types, and the three strains have potential interaction, can be mutually matched to improve the physicochemical property of soil, improve the nitrogen, phosphorus, potassium, calcium and organic matter nutrients of the soil, and simultaneously improve the biological effective state content of mineral elements in the soil, improve the soil nutrition and provide sufficient nutrient supply for the growth of crops. Can promote the growth of crops, obviously increase the plant height, root length, dry weight and fresh weight of plants, effectively improve the contents of mineral elements such as calcium, iron, zinc, selenium and the like of plants, and obviously improve the nutrition, health and quality of crops.

Inventors

  • QU JIANHANG
  • ZHAI MEI
  • HUANG FENG
  • ZHAO LEIZHEN
  • ZHAO SHUAI
  • ZHOU JIA

Assignees

  • 河南工业大学

Dates

Publication Date
20260512
Application Date
20251106

Claims (8)

  1. 1. The nitrogen-phosphorus-potassium composite microbial inoculum is characterized in that a bacterial strain in the nitrogen-phosphorus-potassium composite microbial inoculum consists of Pseudomonas 1416X3 with a preservation number of CGMCC No. 17557, proteus (Priestia sp.) ST2 with a preservation number of CGMCC No. 36011 and Burkholderia YHN3-13 with a preservation number of CGMCC No. 36012.
  2. 2. The nitrogen-phosphorus-potassium composite microbial agent according to claim 1, wherein the ratio of the viable count of Pseudomonas 1416X3, privet bacteria (Priestia sp.) ST2 and Burkholderia (Burkholderia sp.) YHN3-13 is (1-3): 1-3.
  3. 3. The preparation method of the nitrogen-phosphorus-potassium composite microbial inoculum according to claim 1 or 2 is characterized in that pseudomonas 1416X3, proprietaria ST2 and Burkholderia YHN3-13 are respectively inoculated in a liquid culture medium to be cultured to a logarithmic growth phase, the OD 600 value of the bacterial strain is respectively regulated to 0.9-1.0 by using sterile physiological saline, and the bacterial strain is prepared by mixing the bacterial strain according to the volume ratio of (1-3) to (1-3).
  4. 4. The preparation method of the nitrogen-phosphorus-potassium composite microbial inoculum according to claim 3, which is characterized in that the liquid culture medium is LB liquid culture medium, the culture condition is that the temperature is 28-30 ℃, the stirring speed is 150-180 r/min, and the culture is 12 h.
  5. 5. Use of the nitrogen-phosphorus-potassium composite microbial inoculum according to claim 1 or 2 for promoting plant growth.
  6. 6. The use of the nitrogen-phosphorus-potassium composite microbial inoculum according to claim 1 or 2 for increasing the nitrogen, phosphorus, potassium and organic matter contents in soil.
  7. 7. Use of the nitrogen-phosphorus-potassium composite microbial agent of claim 1 or 2 for improving the bioavailability of calcium, iron, zinc, manganese, molybdenum and selenium elements in soil.
  8. 8. The use of the nitrogen, phosphorus and potassium composite microbial inoculum according to claim 1 or 2 for improving the bioavailability of calcium, iron, zinc, manganese, molybdenum and selenium elements in soil while improving the contents of nitrogen, phosphorus, potassium and organic matters in the soil.

Description

Nitrogen-phosphorus-potassium composite microbial inoculant, and preparation method and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a nitrogen-phosphorus-potassium composite microbial agent, a preparation method and application thereof. Background With the rise of the demand for sustainable development of global agriculture, the problems of soil degradation, environmental pollution and agricultural product safety caused by long-term use of chemical fertilizers and pesticides are increasingly prominent. Soil health is a cornerstone of nutritional health of agricultural products and human life health. The microbial agent is an important research direction for green agricultural development due to the characteristics of being ecological, low in cost, easy to produce and the like. The microorganism can utilize self life metabolism to realize multiple effects such as conversion and supply of nutrient elements such as nitrogen, phosphorus, potassium, etc., to generate plant growth regulating substances such as auxin (IAA), gibberellin (GA), etc., to generate antibacterial substances to inhibit pathogenic bacteria, relieve biological stress, etc. However, the current microbial agent products and applications still have the problems of single function of strains, poor environmental adaptability, unstable effect and the like. And the research and development of the microbial agent aiming at activating the trace elements of the soil are relatively weak. Microelements play an irreplaceable role in key physiological processes of plant enzyme system activation, photosynthesis, carbohydrate transportation, nitrogen fixation, metabolism and the like, and are transmitted to a dining table through agricultural products such as crop fruits and the like to influence the life health of human beings. However, many trace elements in the soil exist in a relatively immobilized state with low solubility, are difficult to be directly absorbed and utilized by plants, and restrict the improvement of crop yield and nutrition quality. The composite microbial agent can exert various functions of nitrogen fixation, phosphorus dissolution, potassium dissolution, auxin secretion and the like while enhancing the environmental adaptability through scientific compatibility of various functional strains, and improves the nutrient utilization efficiency, thereby showing remarkable advantages. Reference 1 chinese patent document publication No. CN116814457 a. Reference 1 describes a growth-promoting bacterium, a composite bacterium, a microbial inoculum, a bacterial fertilizer and applications thereof, including Burkholderia pyrrochanteria and Pseudomonas aragonii, wherein the Burkholderia pyrrochanteria is F JS-3 which is stored in the microorganism strain collection of Guangdong province, the storage date is 2022, 7 months and 20 days, the storage number is GDMCCNo, 62432, the Pseudomonas aragonii is Pseudomonas aragonii (Pseudomonas atagonensis) F JS-16 which is stored in the microorganism strain collection of Guangdong province, the storage date is 2022, 7 months and 20 days, and the storage number is GDMCCNo, 62433. The growth-promoting compound bacteria have better growth-promoting effect on plants. The existing microbial inoculum is focused on solving the problem of supplying large amounts of elements such as nitrogen, phosphorus, potassium and the like in soil, and the functional positioning is relatively single. The method has limited or unreported effect on comprehensively and systematically improving the comprehensive fertility of the soil, in particular to synchronously activating medium and trace elements (such as calcium, iron, zinc, manganese, molybdenum, selenium and the like) in the soil. The low bioavailability of medium trace elements, which are critical to the quality formation, stress resistance and human health of crops, has become one of the key bottlenecks limiting the production of high quality agricultural products. Therefore, the invention aims to provide a brand new compound microbial agent and application thereof, aiming at the defects of the existing microbial agent in aspects of functional comprehensiveness, quality improvement potential and application broad spectrum. The microbial inoculum not only can effectively improve the nitrogen, phosphorus, potassium and organic matter content of soil and realize weight loss and yield increase, but also has the core advantages of being capable of remarkably improving the bioavailability of various key medium trace elements (calcium, iron, zinc, manganese, molybdenum and selenium) in the soil, thereby radically improving the nutrition of crops, remarkably improving the nutrition quality of agricultural products while improving the yield, and showing good applicability across crops. Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide a nitrogen-phosphorus-potassi