CN-122012267-A - Efficient corn straw composite degradation bacterium DF and application thereof

Abstract

The invention discloses a high-efficiency corn stalk composite degradation bacterium DF and application thereof, and relates to the technical field of microorganisms, wherein the composite degradation bacterium DF is formed by bacillus belicus (Bacillus velezensis) HW-124 and bacillus amyloliquefaciens (Bacillus Amyloliquefaciens) HW-130, the preservation number of bacillus belicus (Bacillus velezensis) is CGMCC No.7.626, the preservation number of bacillus amyloliquefaciens (Bacillus Amyloliquefaciens) HW-130 is CGMCC No.7.625, and the bacillus belicus HW-124 and bacillus amyloliquefaciens HW-130 have the capability of efficiently degrading corn stalks, can cooperatively play a role, obviously improve the straw utilization rate and play a role of promoting straw returning.

Inventors

• MI JUNZHEN
• WANG PEIXIN
• CHEN XIAOJING
• LIU JINGHUI
• ZHAO BAOPING
• REN WENHUI
• WANG JINJIN
• ZHANG JUNZHEN
• WANG XIQUAN
• XU ZHONGSHAN
• XUE YAQI

Assignees

• 内蒙古农业大学

Dates

Publication Date

20260512

Application Date

20251215

Claims (4)

1. 1. A high-efficiency corn stalk composite degradation bacterium DF is characterized in that the composite degradation bacterium DF consists of bacillus belicus (Bacillus velezensis) HW-124 and bacillus amyloliquefaciens (Bacillus Amyloliquefaciens) HW-130; The bacillus belicus (Bacillus velezensis) HW-124 is preserved in China general microbiological culture collection center (CGMCC), the preservation number is CGMCC NO.7.626, and the preservation date is 2025, 12 and 3; The bacillus amyloliquefaciens (Bacillus Amyloliquefaciens) HW-130 is preserved in China general microbiological culture Collection center (CGMCC), the preservation number is CGMCC NO.7.625, and the preservation date is 2025, 12 and 3.
2. 2. The efficient corn stalk composite degrading bacterium DF according to claim 1, wherein the composite ratio of bacillus belicus (Bacillus velezensis) HW-124 to bacillus amyloliquefaciens (Bacillus Amyloliquefaciens) HW-130 is 1:1.
3. 3. Use of the composite degrading bacterium DF according to any one of claims 1-2 for degrading crop straw.
4. 4. The application of the composite degrading bacteria DF in degrading crop straws according to claim 3, wherein the crops comprise corn, rice, wheat, potato and sorghum.

Description

Efficient corn straw composite degradation bacterium DF and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a high-efficiency corn stalk composite degradation bacterium DF and application thereof. Background Corn is a first grain crop planted in successive years in China, the generated corn straw amount occupies a relatively high proportion in the total crop straw resources, and the actual utilization rate is only about 40% although the corn straw resources are huge. The corn straw contains rich lignocellulose, mainly comprises cellulose, hemicellulose and lignin, if the corn straw can be fully degraded, the corn straw can be decomposed into micromolecular substances and nutrient element components which are returned to soil to be absorbed and utilized by crops, and sustainable recycling of resources is realized, but the lignocellulose is mutually embedded in a structure, has a complex configuration and is difficult to be fully degraded under the condition of natural returning to the field, so that the straw is difficult to be fully degraded, and the low comprehensive utilization rate is a key problem to be solved in the current practical agricultural production. The biodegradation means is to destroy the main structure of lignocellulose by utilizing microorganisms so as to achieve the aim of promoting straw degradation, and in the biodegradation process, the method not only can rapidly and efficiently achieve ideal degradation effect, but also has environmental protection effect, avoids pollution possibly existing in chemical treatment, and saves manpower and mechanical utilization degree of physical treatment. Compared with a single strain, the degradation capability of the composite constructed bacterial system on lignocellulose in the straw can be obviously improved, and the promotion effect on straw degradation is more obvious. The screening of the degradation composite bacterial group is of vital importance in the research of crop straw degradation, and is also an indispensable means for promoting the effective utilization of crop straw resources. Therefore, it is necessary to screen and culture the composite bacterial group with the efficient straw degradation performance so as to solve the problems. Disclosure of Invention The invention aims to solve the technical problems of low utilization rate and environmental pollution in the straw treatment mode in the prior art, and provides a high-efficiency corn straw composite degradation bacterium DF and application thereof. The technical scheme for solving the technical problems is that the efficient corn stalk composite degradation bacteria DF are obtained by preserving bacillus bailii (Bacillus velezensis) HW-124 and bacillus amyloliquefaciens (Bacillus Amyloliquefaciens) HW-130, wherein the bacillus bailii (Bacillus velezensis) HW-124 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC NO.7.626 and the preservation date of 2025 and 12 months and 3 days, and the bacillus amyloliquefaciens (Bacillus Amyloliquefaciens) HW-130 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC NO.7.625 and the preservation date of 2025 and 12 months and 3 days. Further, the compounding ratio of bacillus belicus (Bacillus velezensis) HW-124 to bacillus amyloliquefaciens (Bacillus Amyloliquefaciens) HW-130 is 1:1. Further, the application of the composite degrading bacteria DF in degrading crop straws. Further, the crops include corn, rice, wheat, potato and sorghum. The invention has the beneficial effects that the bacillus belicus (Bacillus velezensis) HW-124 and the bacillus amyloliquefaciens (Bacillus Amyloliquefaciens) HW-130 are adopted to act on the straws through the synergistic effect, so that the straw degradation rate can be obviously improved, the degradation process of the straws is that the composite bacterial liquid prepared by mixing the two single bacterial liquid 1:1 is inoculated into an enzyme-producing liquid culture medium to prepare a bacterial liquid with a certain concentration and uniformly contact the surface of the treated straws, the bacillus belicus HW-124 and the bacillus amyloliquefaciens HW-130 have the capability of efficiently degrading corn straws, the synergistic effect can be realized, the straw utilization rate can be obviously improved, and the effect of promoting the straw returning can be realized. Drawings FIG. 1 is a graph of degradation rate for a single strain HW-124 straw fermentation test of the present invention. FIG. 2 is a graph of degradation rate for a single strain HW-130 straw fermentation test. FIG. 3 is a bar graph of the change in the activity of the complex DF cellulase. Fig. 4 is a graph of degradation rate of corn stalks in a composite DF potting test. FIG. 5 is a graph of the degradation rate of corn stalks in a co