CN-122012268-A - Composite microbial inoculum and application thereof in improving saline-alkali soil and promoting alfalfa growth

Abstract

The invention relates to the technical field of microorganisms, in particular to a composite microbial inoculum and application thereof in improving saline-alkali soil and promoting alfalfa growth. The composite microbial inoculum comprises pickled vegetable bacillus megatherium HM-1 and salt-tolerant nitrogen-fixing blue algae HH0518. The invention provides an economic, feasible and efficient bioremediation method for improving saline-alkali soil, provides a new biotechnology path for agricultural ecological remediation, and has important application value for realizing the efficient utilization of saline-alkali soil resources and the agricultural green development by jointly applying the pickle ocean bacillus HM-1 and the salt-tolerant nitrogen-fixing blue algae HH0518 algae strain and improving the crop growth and soil health by adopting the synergistic effect of a biological symbiotic system, and has the advantages of greenness, low consumption, sustainability and the like.

Inventors

• ZHAO CHENCHEN
• Liu Ruoyan
• ZHANG FENGLAN
• ZHANG YUMING
• WEI BIN
• WANG ZHONGQI
• LIU GUIXIA
• LIU FENGSONG
• CAO DANDAN

Assignees

• 河北大学

Dates

Publication Date

20260512

Application Date

20251222

Claims (10)

1. 1. A composite microbial inoculum is characterized by comprising pickled vegetable bacillus megatherium (Oceanobacillus kimchii) HM-1 and salt-tolerant nitrogen-fixing blue algae (Leptolyngbya angustata) HH0518, wherein the preservation number of the pickled vegetable bacillus megatherium HM-1 is CGMCC No.30790, and the preservation number of the salt-tolerant nitrogen-fixing blue algae HH0518 is CGMCC No. 41195.
2. 2. The composite microbial inoculant according to claim 1, wherein the mass ratio of the pickle bacillus megatherium HM-1 to the salt-tolerant nitrogen-fixing cyanobacteria HH0518 is 1 (1-2).
3. 3. The composite microbial inoculant according to claim 2, wherein the mass ratio of the pickled vegetable bacillus megatherium HM-1 to the salt-tolerant nitrogen-fixing cyanobacteria HH0518 is 1 (1.8-2).
4. 4. A fertilizer additive or a fertilizer, characterized in that it contains the composite microbial agent of any one of claims 1 to 3.
5. 5. A soil conditioner comprising the composite microbial inoculum according to any one of claims 1 to 3.
6. 6. Use of the composite microbial inoculant of any one of claims 1-3, the fertilizer additive or fertilizer of claim 4, or the soil conditioner of claim 5 for improving saline-alkali soil and/or promoting alfalfa growth.
7. 7. The use according to claim 6, wherein the promotion of alfalfa growth is characterized by at least one of increasing alfalfa plant height, increasing alfalfa fresh weight, increasing alfalfa dry weight, increasing alfalfa proline content, decreasing alfalfa malondialdehyde content, decreasing alfalfa catalase activity, increasing alfalfa chlorophyll content; And/or the modified saline-alkali soil is characterized by at least one of reducing the conductivity of the soil and improving the quick-acting nitrogen content in the soil.
8. 8. The use according to claim 6 or 7, wherein the promotion of alfalfa growth is promotion of alfalfa growth under the saline-alkali stress of saline-alkali soil.
9. 9. The use according to claim 6 or 7, wherein the alfalfa is alfalfa.
10. 10. The application of the composite microbial inoculant of any one of claims 1-3 in preparing saline-alkali soil modifier or saline-alkali stress resistant plant promoter or saline-alkali stress resistant fertilizer.

Description

Composite microbial inoculum and application thereof in improving saline-alkali soil and promoting alfalfa growth Technical Field The invention relates to the technical field of microorganisms, in particular to a composite microbial inoculum and application thereof in improving saline-alkali soil and promoting alfalfa growth. Background Soil salinization is one of the main ecological problems which restrict the sustainable development of agriculture in the current global scope. Salinization can lead to deterioration of soil structure, nutrient loss, reduced aeration and water permeability and reduced microbial activity, thereby seriously affecting the growth and yield of crops. The saline-alkali soil has high salt concentration and alkaline pH value, which causes unbalanced osmotic pressure, nutrient absorption disorder and physiological interference of plant root system, and causes the problems of low survival rate, small biomass, reduced quality and the like of crops. Alfalfa is used as a high-quality perennial leguminous forage grass, has stronger nitrogen fixation capability of root systems and higher nutritive value, and is widely used for feed production and soil improvement. However, in the saline-alkali environment, alfalfa growth is significantly inhibited, and the phenomena of low emergence rate, reduced biomass, reduced protein content and the like are presented. This not only affects the yield and quality of alfalfa, but also limits its potential for application in saline-alkali soil improvement and animal husbandry development. In order to improve the saline-alkali soil environment and plant growth performance, students at home and abroad have developed various improvement technical researches including chemical improvement (such as gypsum application, acidic substances and the like), physical improvement (such as salt elimination and washing) and biological improvement (such as soil improvement by utilizing a microorganism and plant symbiotic system). Among them, the microorganism improvement method is receiving attention because of its advantages of green, sustainable, eco-friendly, etc. The bacteria can secrete organic acid, extracellular polysaccharide and biomass to improve physical and chemical properties of soil and promote plant growth, and the microalgae can fix carbon dioxide, release oxygen and improve microecological activity of soil through photosynthesis. However, the current research on the application of bacteria and microalgae in combination to saline-alkali soil improvement and alfalfa growth promotion is still under investigation. Most of the related bacteria and algae are separated from neutral soil environment, and when the bacteria and algae are applied to saline-alkali soil improvement, the defects of weak colonization capability and low survival rate often occur, which greatly influence the effect of the bacteria and algae on the saline-alkali soil improvement. Therefore, the development of the saline-alkali soil biological improvement technology based on the synergistic effect of bacteria and microalgae has important theoretical significance and application value for improving soil environment, promoting healthy growth of alfalfa and realizing efficient utilization of saline-alkali soil resources. Disclosure of Invention In order to solve the technical problems, the invention screens and constructs a compound symbiotic system of salt-tolerant bacteria and salt-tolerant microalgae aiming at the saline-alkali soil environment for the first time. Different from the traditional neutral bacterial algae system, the invention selects the bacterial algae strain with high salt stress tolerance and growth promoting potential, so that the bacterial algae strain can stably grow and colonize in a high-salt and high-pH environment, and the problem of poor viability of the traditional bacterial algae system in saline-alkali soil is effectively solved. The combination of the two can be mutually beneficial and interdependent, thereby more effectively helping alfalfa to absorb soil nutrients required by growth, improving physicochemical properties of saline-alkali soil, promoting alfalfa growth and providing a feasible thought for the research and development of plant growth promoters. In addition, the invention optimizes the proportion and application mode of the algae according to the physiological characteristics of alfalfa that has limited growth in saline-alkali soil. Promoting root system development and improving nutrient absorption capacity through rhizosphere symbiosis, thereby obviously improving germination rate, biomass and protein content of alfalfa. The design not only improves the soil environment, but also gives consideration to the improvement of the quality of feed crops, and has remarkable agricultural application value. Based on this, the following technical scheme is proposed. The invention firstly provides a composite microbial inoculum, which comprises pickled vege