CN-122012270-A - Application of nitrification and denitrification composite microbial inoculum in aquaculture

Abstract

The invention discloses application of a nitrification and denitrification compound microbial inoculum in aquaculture. According to the invention, the acinetobacter rouxii (Acinetobacter lwoffii) C25 and the pseudomonas stutzeri (Stutzerimonas stutzeri) C29 with the nitrification and denitrification functions are obtained through directional screening, and the C25 and the C29 are compounded to prepare the composite microbial inoculum, and the microbial inoculum can efficiently degrade NH 4 + -N、NO 2 ‑ -N and NO 3 ‑ -N, so that rapid and stable denitrification is realized, and the microbial inoculum is safe and harmless to fish bodies. The composite microbial inoculum has wide application prospect in the field of denitrification of water bodies in an aquaculture pond, treatment of tail water of aquaculture or biological denitrification treatment of other nitrogenous sewage.

Inventors

• QIN QIWEI
• XU YUMIN
• SUN HONGYAN
• LUO WULANG
• ZHU ZHENG
• LIN YUNXIANG
• Wei jingguang
• YANG WENFENG

Assignees

• 华南农业大学

Dates

Publication Date

20260512

Application Date

20251231

Claims (10)

1. 1. The nitrification and denitrification composite microbial inoculum is characterized by comprising Acinetobacter reuteri (Acinetobacter lwoffii) and Pseudomonas stutzeri (Stutzerimonas stutzeri).
2. 2. The composite microbial agent according to claim 1, wherein the Acinetobacter reuteri is Acinetobacter reuteri (Acinetobacter lwoffii) C25, the Pseudomonas stutzeri is Pseudomonas stutzeri (Stutzerimonas stutzeri) C29, the Acinetobacter reuteri C25 is provided with a preservation number of GDMCC NO:67476, and the Pseudomonas stutzeri C29 is provided with a preservation number of GDMCC NO:67477.
3. 3. The composite microbial agent of claim 2, wherein the composite microbial agent is prepared by compounding Acinetobacter rouxii C25 and Pseudomonas stutzeri C29 according to a mass ratio of 1:1.
4. 4. The preparation method of the composite microbial inoculum according to claim 1,2 or 3 is characterized by comprising the steps of respectively inoculating Acinetobacter rouxii and Pseudomonas stutzeri into a liquid culture medium for shake culture to obtain corresponding bacterial solutions, and then mixing the two bacterial solutions to obtain the composite microbial inoculum.
5. 5. The method of claim 4, wherein the liquid medium is LB liquid medium.
6. 6. The method according to claim 4, wherein the shake culture is continued until the strain enters a logarithmic growth phase, and wherein the shake culture is carried out at a temperature of 28℃and a rotation speed of 200rpm.
7. 7. The method of claim 4, wherein the two bacteria are mixed in a 1:1 ratio.
8. 8. Use of the composite microbial agent of claim 1, 2 or 3 in water purification.
9. 9. The use according to claim 8, wherein the use is of a complex microbial agent in denitrification of aquaculture water, preferably the complex microbial agent is used to achieve denitrification of water by degrading NH 4 + -N、NO 2 - -N and NO 3 - -N.
10. 10. A method for denitrification of water body is characterized in that the composite microbial inoculum of claim 1,2 or 3 is added into the water body to be treated, and the denitrification of the water body is realized by degrading NH 4 + -N、NO 2 - -N and NO 3 - -N.

Description

Application of nitrification and denitrification composite microbial inoculum in aquaculture Technical Field The invention relates to the technical field of microorganisms, in particular to application of a nitrification and denitrification compound microbial inoculum in aquaculture. Background The aquaculture water environment is an essential survival factor of aquatic economic animals, and the quality condition of the aquatic economic animals is directly related to the health of fish bodies. The culture environment is often in a relatively closed state, and a large amount of nitrogen in the water body is accumulated easily to exceed the tolerance range of aquatic animals, so that a stress effect is formed. Such stress not only causes physiological dysfunction, but also exacerbates the growth and spread of pathogenic microorganisms, thereby inducing large-scale disease outbreaks. Therefore, how to effectively alleviate the negative influence of nitrogen stress on farmed animals is a key problem to be solved urgently in the current aquaculture industry. The use of microorganisms for denitrification of water environments has been an important technical direction for improving the cultivation environment. The nitrifying and denitrifying bacteria can effectively degrade high-concentration nitrogen, so that the harm of nitrogen stress to cultured animals is relieved. Compared with single strain, the compound microorganism has the advantages of multiple functions, high efficiency of promotion and effectiveness, strong stability and the like. Based on the method, the separated bacterial strains are screened from the aquaculture water environment, the composite microbial flora with high-efficiency denitrification capability is constructed, and the microbial flora is developed into a microbial preparation suitable for being put into aquaculture water, and has important significance for healthy aquaculture and disease prevention and control of aquatic animals. Disclosure of Invention The invention provides an application of a nitrifying and denitrifying composite microbial inoculum in aquaculture, which comprises Acinetobacter rouxii (Acinetobacter lwoffii) and Pseudomonas stutzeri (Stutzerimonas stutzeri) to form the composite microbial inoculum, and NH 4+-N、NO2- -N and NO 3- -N are efficiently degraded through synergistic effect, so that rapid nitrogen reduction is realized, and the problems of low degradation efficiency and poor stability existing in the prior art that a single strain is used for denitrification are solved. The first object of the invention is to provide a nitrifying and denitrifying composite microbial agent, which consists of Acinetobacter reuteri (Acinetobacter lwoffii) and Pseudomonas stutzeri (Stutzerimonas stutzeri). Preferably, the acinetobacter rouxii is acinetobacter rouxii (Acinetobacter lwoffii) C25, and the pseudomonas stutzeri is pseudomonas stutzeri (Stutzerimonas stutzeri) C29. Acinetobacter reuteri (Acinetobacter lwoffii) C25 is deposited at the microorganism strain collection (GDMCC) of Guangdong province at 12 months of 2025, address: floor 5 No. 59 in Guangzhou province martyr, post code: 510070, deposit number: GDMCC NO:67476, and Pseudomonas stutzeri (Stutzerimonas stutzeri) C29 is deposited at the microorganism strain collection (GDMCC) of Guangdong at 12 months of 2025, address: floor 5 No. 59 in Guangzhou province martyr, post code: 510070, deposit number: GDMCC NO:67477. Further preferably, the composite microbial inoculum is prepared by compounding Acinetobacter rouxii C25 and Pseudomonas stutzeri C29 according to the mass ratio of 1:1. The second object of the invention is to provide a preparation method of the nitrifying and denitrifying composite microbial inoculum, which comprises the following steps of respectively inoculating Acinetobacter rouxii and Pseudomonas stutzeri into a liquid culture medium for shake culture to obtain corresponding microbial inoculum, and then mixing the two microbial inoculum to obtain the composite microbial inoculum. Preferably, the liquid medium is LB liquid medium. Preferably, the shake culture is continued until the strain enters the logarithmic growth phase. Preferably, the shake culture condition is that the temperature is 28 ℃ and the rotating speed is 200rpm. Preferably, the two bacteria are mixed according to the mass ratio of 1:1 to prepare the composite bacterial agent. The third purpose of the invention is to provide the application of the nitrification and denitrification composite microbial inoculum in water purification. Preferably, the application is the application of the composite microbial inoculum in denitrification of the culture water body. The composite microbial inoculum has denitrification function and high-efficiency denitrification capability. Further preferably, the composite microbial inoculum is used for realizing denitrification of the water body by degrading NH 4+-N、NO2- -N and NO 3- -N. The invention also