CN-122012274-A - Compound microbial agent for overcoming continuous cropping obstacle of pinellia ternate and application thereof

CN122012274ACN 122012274 ACN122012274 ACN 122012274ACN-122012274-A

Abstract

The invention belongs to the field of microorganisms, and in particular relates to a compound microbial agent for overcoming continuous cropping obstacle of pinellia ternate and application thereof. The active ingredients of the composite microbial inoculum consist of Sphingomonas (Sphingomonas kyungheensis) JN21 (CGMCC No. 36292) and cold-resistant bacillus proximal (Peribacillus frigoritolerans) F16 (CGMCC No. 36291) of the university of Qingxi. Both strains can effectively degrade the key continuous cropping autotoxic substance of pinellia ternate, p-hydroxybenzoic acid. The compound microbial inoculum is applied to the pinellia ternate continuous cropping soil, so that the content of parahydroxybenzoic acid in the soil is obviously reduced, the carbon, nitrogen and sulfur circulation in the soil is promoted, and especially the abundance of genes of a hydrogen sulfide production path is promoted, thereby relieving or preventing the pinellia ternate continuous cropping obstacle. The invention provides an effective microorganism solution for solving the problem of the continuous cropping obstacle of pinellia ternate.

Inventors

SHEN XIHUI
CHENG QI
DU ZHINAN
GUO SHENGZHI
WANG YAO
LI XIANYU

Assignees

西北农林科技大学

Dates

Publication Date: 20260512
Application Date: 20260122

Claims (10)

1. The composite microbial inoculum is characterized in that active ingredients of the microbial inoculum are Sphingomonas celebrata Sphingomonas kyungheensis JN and cold-resistant bacillus parapsilosis Peribacillus frigoritolerans F at university of Qingxi, the preservation number of Sphingomonas celebrata Sphingomonas kyungheensis JN at university of Qingxi is CGMCC No.36292, and the preservation number of the cold-resistant bacillus parapsilosis Peribacillus frigoritolerans F is CGMCC No.36291.
2. A strain for preparing the composite microbial agent as defined in claim 1, wherein the strain comprises sphingomonas at university of gentamicin Sphingomonas kyungheensis JN, and the preservation number of sphingomonas at university of gentamicin Sphingomonas kyungheensis JN is CGMCC No.36292.
3. The strain according to claim 2, wherein the university of gentamicin Sphingomonas Sphingomonas kyungheensis JN, is degradable for p-hydroxybenzoic acid.
4. A strain for preparing the composite microbial agent of claim 1, wherein the strain comprises bacillus thuringiensis Peribacillus frigoritolerans F and the preservation number of bacillus thuringiensis Peribacillus frigoritolerans F is CGMCC No.36291.
5. The strain of claim 4, wherein the Bacillus thuringiensis 3835.cold-resistant strain Peribacillus frigoritolerans F is capable of degrading p-hydroxybenzoic acid.
6. The use of the composite microbial agent of claim 1 for degrading p-hydroxybenzoic acid.
7. The use of the complex microbial agent of claim 1 for alleviating or preventing pinellia ternate continuous cropping obstacle.
8. The use according to claim 7, wherein said alleviation or prevention is achieved by degradation of an autotoxic substance in the pinellia ternate continuous cropping soil by application of said complex inoculant in the pinellia ternate continuous cropping soil, said autotoxic substance being p-hydroxybenzoic acid.
9. The use according to claim 7, wherein said alleviation or prevention is achieved by promoting carbon nitrogen sulfur cycle in pinellia ternate continuous cropping soil by applying said complex microbial agent in pinellia ternate continuous cropping soil, said promotion of carbon nitrogen sulfur cycle comprising increasing abundance of genes associated with hydrogen sulfide production pathway in pinellia ternate continuous cropping rhizosphere soil.
10. A method of preparing the composite microbial inoculant of claim 1, comprising the steps of: S1, respectively culturing Sphingomonas celebrata Sphingomonas kyungheensis JN and cold-resistant bacillus parapsilosis Peribacillus frigoritolerans F in an LB culture medium to obtain a bacterial suspension of Sphingomonas celebrata Sphingomonas kyungheensis JN and a bacterial suspension of cold-resistant bacillus parapsilosis Peribacillus frigoritolerans F; S2, mixing the bacterial suspension of the Sphingomonas Sphingomonas kyungheensis JN at the university of Qingxi and the bacterial suspension of the cold-resistant bacillus paradise Peribacillus frigoritolerans F prepared in the step S1 to obtain the composite bacterial agent.

Description

Compound microbial agent for overcoming continuous cropping obstacle of pinellia ternate and application thereof Technical Field The invention belongs to the field of microorganisms, and in particular relates to a compound microbial agent for overcoming continuous cropping obstacle of pinellia ternate and application thereof. Background The main reasons for the occurrence of the pinellia ternate continuous cropping obstacle include unbalance of a soil microbiota, accumulation of chemosensory autotoxic substances and deterioration of physical and chemical properties of soil. The plant rhizosphere microorganisms are microorganism groups which are closely adhered to the surface of a plant root system or inhabit soil around the root system and are in a specific micro-ecological environment influenced by root secretions, falling objects and the like, and can supply growing microorganisms by taking allelochemically-perceived autotoxic substances as carbon sources. However, the current utilization of rhizosphere microorganisms remains more in artificially inoculated single species, often neglecting interactions between strains and their synergistic use. The strains of the complex microbial agents are derived from key functional species symbiotic with the dominant plants, in particular plant rhizosphere microorganisms. Thus, the strains of the composite microbial agents have many advantages over the natural microbial community and single strains. Firstly, the strain in the composite microbial agent can play a weighted effect, so that the microbial group can be utilized more simply and efficiently, secondly, the composite microbial agent can optimize the metabolic path and simultaneously execute multiple functions, reduce the burden of cell metabolism and have better degradation effect on chemosensory autotoxic substances. At present, reports on a pinellia ternate chemosensory autotoxic substance degrading bacteria compound bacterial agent are not yet seen, so that strains capable of degrading chemosensory autotoxic substances in soil are excavated and the compound bacterial agent is constructed, so that continuous cropping obstacles of pinellia ternate are relieved, and the method has important significance for enhancing artificial planting and sustainable development of the pinellia ternate. Disclosure of Invention In order to solve the technical problems, the invention screens and obtains the Sphingomonas Sphingomonas kyungheensis JN and the cold-resistant bacillus parasporium Peribacillus frigoritoleransF16 from the rhizosphere soil of pinellia ternate, cultures and obtains bacterial suspensions of Sphingomonas parasporium Sphingomonas kyungheensis JN and the cold-resistant bacillus parasporium Peribacillus frigoritoleransF of the university, and mixes the bacterial suspensions to prepare the composite microbial inoculum, the composite microbial inoculum is applied to the continuous cropping soil of the pinellia ternate, the content of the pinellia ternate allelopathy autotoxic substance-p-hydroxybenzoic acid in the continuous cropping soil of the pinellia ternate is obviously reduced, and the absolute quantification of the genome of the rhizosphere soil of the continuous cropping soil of the pinellia ternate is detected, in addition, the composite microbial inoculum promotes the carbon-nitrogen-sulfur circulation in the continuous cropping soil of the pinellia ternate, and particularly improves the genes producing hydrogen sulfide pathways to promote the growth of the pinellia ternate in the continuous cropping in-situ soil. In one aspect, the invention provides a composite microbial inoculum, active ingredients of the microbial inoculum are Sphingomonas celebrata Sphingomonas kyungheensis JN and cold-resistant bacillus parapsilosis Peribacillus frigoritoleransF at university, the preservation number of Sphingomonas celebrata Sphingomonas kyungheensis JN at university is CGMCC No.36292, and the preservation number of the cold-resistant bacillus parapsilosis Peribacillus frigoritoleransF is CGMCC No.36291. In a second aspect, there is also provided a strain for preparing the composite microbial inoculum of the invention, wherein the strain comprises Sphingomonas sp Sphingomonas kyungheensis JN of the university of Qingxi, and Sphingomonas sp Sphingomonas kyungheensis JN of the university of Qingxi is obtained by screening from pinellia rhizosphere soil, and the preservation information is as follows: strain name JN21; classification name, sphingomonas Sphingomonas kyungheensis, university of gentamicin; The collection time is 2025, 10 months and 22 days; The release time of the preservation certificate is 2025, 11 and 05; the preservation unit is China general microbiological culture Collection center (CGMCC); the preservation number is CGMCC No.36292. Address of the institute of microbiology of the national academy of sciences of China, the Korean area North Star, the West way 1, the 3 rd. Further, the universi