CN-122012275-A - Fermentation method of nitrifying bacteria consortium based on microelement regulation and control

Abstract

The invention relates to the technical fields of environmental microorganisms and fermentation engineering, in particular to a fermentation method of nitrifying bacteria consortium based on microelement regulation. And inoculating the seed liquid into a fermentation culture medium for fermentation, adding trace element feed liquid according to an oxygen consumption rate value in the fermentation process, and carrying out stage feed supplementing to form a mixed fermentation system with synchronous and stable growth of the AOB and the NOB, thereby solving the problems of unstable fermentation and no nitrite nitrogen degradation capability of nitrifying bacteria, reducing the fermentation time of a single tank and improving the ammonia oxidation rate of a finished product.

Inventors

• FAN SHUAI
• WU DINGXIN
• ZHU HAI
• Tan Yinping

Assignees

• 武汉水之国环保科技有限公司

Dates

Publication Date

20260512

Application Date

20260122

Claims (10)

1. 1. A method for fermenting a nitrifying bacterial consortium, comprising the steps of: s1, culturing nitrifying bacteria consortium into fermentation seed liquid, wherein the nitrifying bacteria consortium is a consortium formed by ammonia oxidizing bacteria AOB and nitrous acid oxidizing bacteria NOB; s2, inoculating the seed liquid into a fermentation medium for fermentation, adding a feed liquid according to an oxygen consumption rate value in the fermentation process, and carrying out stage feed: Step one, when the oxygen consumption rate value is less than 40mg O 2 /(gVSS.h), batch feeding is carried out; Step two, when the oxygen consumption rate reaches 40mg O 2 /(gVSS.h), starting automatic feeding; Step three, when the oxygen consumption rate value is more than 40mg O 2 /(gVSS.h), starting the speed-up automatic feeding; And in the fourth stage, when the fermentation reaches the oxygen consumption rate value of less than 40mg O 2 /(gVSS.h), stopping feeding and stopping fermentation.
2. 2. The method for fermenting a nitrifying bacteria consortium as set forth in claim 1, wherein in step S1, the ammoxidation rate of the consortium in the fermentation seed liquid is 50 to 60 mgNH 3 -N/L/hr.
3. 3. The method for fermenting a nitrifying bacteria consortium as set forth in claim 1, wherein in step S2, the feed liquid contains Fe, cu, ca and Se elements.
4. 4. A fermentation method of a nitrifying bacteria consortium as set forth in claim 3, wherein the molar concentration of the Fe element is 144 to 216. Mu. Mol/L, the molar concentration of the Cu element is 8 to 20. Mu. Mol/L, the molar concentration of the Ca element is 13.5 to 20.5. Mu. Mol/L, and the molar concentration of the Se element is 0.75 to 2. Mu. Mol/L.
5. 5. The method for fermenting a nitrifying bacteria consortium as set forth in claim 3, wherein said feed solution further comprises Mn, co and Zn elements, and inorganic salts.
6. 6. The method for fermenting a nitrifying bacteria consortium as set forth in claim 1, wherein in the step S2, the flow rate of the automatic feed in the step two is 38-42 mL/h.
7. 7. The method for fermenting a nitrifying bacteria consortium as set forth in claim 1, wherein in the step S2, the speed of the automatic speed-increasing feed in the step three is 40-80 mL/h, and the feeding time interval is 23-25 h.
8. 8. The method for fermenting a nitrifying bacteria consortium as set forth in claim 7, wherein both ammoniacal nitrogen and nitrite nitrogen in said accelerated automatic feed process are <5mg/L.
9. 9. The method for fermenting a nitrifying bacteria consortium as set forth in claim 1, wherein in step S2, the mark for stopping the feeding in the stage four further comprises a feeding flow rate of 600mL/h.
10. 10. The method for fermenting a nitrifying bacteria consortium as set forth in claim 1, wherein in the step S2, the pH in the fermentation process is 7.0 to 8.0.

Description

Fermentation method of nitrifying bacteria consortium based on microelement regulation and control Technical Field The invention relates to the technical fields of environmental microorganisms and fermentation engineering, in particular to a fermentation method of nitrifying bacteria consortium based on microelement regulation. Background Autotrophic nitrifying bacteria (NITRIFYING BACTERIA) are used as a mature product in the sewage treatment industry. Bacteria that oxidize ammonia nitrogen or ammonium salts to nitrite or nitrite include Ammonia Oxidizing Bacteria (AOB) (e.g., nitromonas, nitrococcus) and Nitrite Oxidizing Bacteria (NOB) (e.g., nitrobacter, nitrococcus). Under the aerobic condition, ammonia is oxidized into nitrite by ammonia oxidizing bacteria, and then nitrite is oxidized into nitrate by nitrite oxidizing bacteria, so that the ammonia oxidizing bacteria and the nitrite cooperate to complete the whole nitration process. Different from the traditional heterotrophic nitrifying microorganism, the autotrophic nitrifying bacteria has 103-106 times of nitrifying capacity and is a core component of natural nitrifying effect. Regarding nitrifying bacteria, the ammoxidation rate is a core standard for evaluating the nitrifying action of the nitrifying bacteria, and the strength of the nitrifying bacteria in the practical application scene is fed back to a certain extent. HG/T5748-2020, which is returned by the national institute of chemical standardization and technology (China) for water treatment and separation technical Committee, further confirms that ammonia oxidation performance, namely the quality of ammonia nitrogen oxidation per unit volume of nitrifying bacteria in unit time under aerobic conditions, is used for evaluating the nitrifying capacity of nitrifying bacteria. The ammonia oxidation performance of nitrifying bacteria is closely related to a fermentation process, but nitrifying bacteria are strictly inorganic autotrophic microorganisms, related researches at home and abroad have inorganic nutrient substances as substrates, and the fermentation process of nitrifying bacteria is mainly different from heterotrophic bacteria in that 1, the nitrifying bacteria are purely inorganic fermentation, the requirements on the fermentation environment are relatively low, 2, in recent years, reports that semi-batch fermentation is often used for improvement, the average generation time of nitrifying bacteria is more than 10 hours, the single-tank fermentation time is usually about 10 days, and 3, nitrifying bacteria comprise Ammonia Oxidizing Bacteria (AOB) and nitrous acid oxidizing bacteria (NOB), but are often used for single-bacterial fermentation of Ammonia Oxidizing Bacteria (AOB). During long-generation and special fermentation modes, nitrifying bacteria fermentation is also accompanied with various problems such as nitrite nitrogen degradation capability of single bacteria fermentation Ammonia Oxidizing Bacteria (AOB) fermentation, but the single bacteria fermentation technology of Nitrite Oxidizing Bacteria (NOB) is deficient, ammonia Oxidizing Bacteria (AOB) fermentation products are severely inhibited, the fermentation concentration is low, the rate is low, a large amount of nitrite nitrogen is generated in tail water, the difficulty in tail water treatment is increased, the fermentation stability is poor, the period is long, mixed bacteria are bred, the data fluctuation is frequent, and the single tank fermentation time is further increased. Therefore, a mixed bacteria fermentation process of autotrophic nitrifying bacteria is needed, and the problems of instability, no nitrite nitrogen degradation capability and the like of nitrifying bacteria fermentation in the current market are solved, so that the single-tank fermentation time is reduced, and the ammonia oxidation rate of a finished product is improved. Disclosure of Invention In view of the above, the invention provides a fermentation method of nitrifying bacteria consortium based on microelement regulation. And in the seed production stage, the AOB and the NOB are spliced to form stable nitrifying bacteria consortium which is used as seed liquid of the upper tank, and the consortium is continuously passaged, so that the abundance of the consortium is increased, the ammoxidation rate of the seed of the upper tank is improved, and the ammoxidation rate of the seed of the upper tank is controlled to be 50-60 mgNH 3 -N/L/hr. In the earlier stage of mixed fermentation, microelement regulation and control are used for promoting NOB proliferation, balancing AOB/NOB biomass, maintaining dynamic growth balance, enabling the nitrite nitrogen production amount in the fermentation process to be smaller than or equal to the NOB proliferation demand amount, further realizing mixed bacteria fermentation balance of nitrifying bacteria, forming a mixed fermentation system with AOB and NOB synchronous stable growth, solving the problems of uns