CN-122012276-A - Compound probiotics preparation for breeding macrobrachium rosenbergii as well as preparation method and application thereof

CN122012276ACN 122012276 ACN122012276 ACN 122012276ACN-122012276-A

Abstract

The invention discloses a compound probiotic preparation for breeding macrobrachium rosenbergii, a preparation method and application thereof, and relates to the technical field of microorganisms, wherein the active strain composition of the compound probiotic preparation comprises lactococcus formans (Lactococcus garvieae), bacillus subtilis (Bacillus subtilis) and nitrifying bacillus vickers (Nitrobacter winogradskyi); wherein the preservation number of the lactococcus formans is CGMCC No.31024. According to physiological requirements of different development stages of larvae in Z1-Z4 stage and Z5-P1 stage, strain proportions of 2:1 and 2:1:1 are adopted to carry out staged accurate addition, artificial seawater with a specific formula is matched, baits such as fairy worms, custard and the like are quantitatively fed according to the development stage, key water quality indexes such as dissolved oxygen, ammonia nitrogen, nitrite and the like of a water body are strictly regulated, a set of green seedling raising technical system integrating probiotics micro-ecological regulation and accurate nutrition supply and water quality management is formed, and finally the effects of improving the survival rate of the larvae, reducing the abnormal deformation rate, promoting the growth and development and replacing antibiotics are realized.

Inventors

GAO QUANXIN
YANG QINGMAN
YI SHAOKUI
Yu Jiongying
TANG QIONGYING
XIA ZHENGLONG
CAI JIUYING

Assignees

湖州师范学院
绍兴市水产技术推广中心
江苏数丰水产种业有限公司

Dates

Publication Date: 20260512
Application Date: 20260123

Claims (10)

1. A compound probiotic preparation for culturing seedlings of macrobrachium rosenbergii is characterized by comprising active strain components of the compound probiotic preparation, wherein the active strain components comprise lactococcus formans (Lactococcus garvieae), bacillus subtilis (Bacillus subtilis) and nitrifying bacillus vickers (Nitrobacter winogradskyi), and the preservation number of the lactococcus formans is CGMCC No.31024.
2. The compound probiotic preparation for breeding macrobrachium rosenbergii according to claim 1, wherein the compound probiotic preparation adopts different strain compositions and proportions according to different development stages of the macrobrachium rosenbergii larvae: in the stages Z1 to Z4 of the macrobrachium rosenbergii larvae, the composite probiotic preparation consists of lactococcus formans and bacillus subtilis, and the ratio of the strains is 2:1; In the stage Z5 to P1 of the macrobrachium rosenbergii larvae, the composite probiotic preparation consists of lactococcus formans, bacillus subtilis and nitrifying bacteria, and the ratio of the strains is 2:1:1.
3. The compound probiotic preparation for breeding macrobrachium rosenbergii according to claim 2, wherein the compound probiotic preparation is diluted with sterile physiological saline before use and activated at room temperature for 10-20 minutes.
4. A preparation method of a compound probiotic preparation for breeding macrobrachium rosenbergii is based on the compound probiotic preparation for breeding macrobrachium rosenbergii of any one of claims 1-3, and is characterized by comprising the steps of, (1) Preparing artificial seawater, wherein each ton of water contains 11000g of sodium chloride, 3700g of magnesium sulfate, 420g of calcium chloride, 190g of potassium chloride, 20g of sodium bicarbonate, 20g of potassium bromide, 120g of boric acid, 6g of EDTA and 1g of strong chlorine, and aerating for 24-48 hours after dissolving; (2) Adding composite probiotics, namely adding the composite probiotic preparation with the corresponding proportion as set forth in claim 2 into a seedling water body according to the development stage of the macrobrachium rosenbergii larvae; (3) Feeding bait, namely feeding artemia nauplii from the stage Z2, adding custard from the stage Z5, and matching shrimp slices from the stage Z11; (4) And (3) water quality regulation: maintain the dissolved oxygen in the water body in the whole course the amount is more than or equal to 5 mg/L, the amount is more than or equal to 5 mg/L of the total number of the components.
5. The method for preparing the compound probiotic preparation for breeding macrobrachium rosenbergii according to claim 4, wherein the adding scheme of the compound probiotic in the step (2) is as follows: Adding once every 2 days in the Z1-Z4 stage to ensure that the final concentration of the lactococcus formans and the bacillus subtilis in the culture water body respectively reaches 1X 10-5 CFU/mL and 5X 10-4 CFU/mL; adding once every 2 days in the stage Z5 to Z9, so that the final concentration of the lactococcus formans, the bacillus subtilis and the nitrifying bacteria in the culture water body respectively reaches 2X 10-5 CFU/mL, 1X 10-5 CFU/mL and 1X 10-5 CFU/mL; In the Z10 to P1 phases, the strains were added once daily, and the final concentration of each strain was the same as in the Z5 to Z9 phases.
6. The method of claim 4, wherein in the step (3), the total daily feeding amount of the artemia is calculated according to the formula of total feeding amount (number) =number of larvae (number of larvae) x daily addition amount per tail (number/number) in the development period, wherein the total daily addition amount of the artemia per tail in each development period is 1-2 in the Z2 period, 2-3 in the Z3-Z4 period, 3-5 in the Z5-Z6 period, 5-8 in the Z7-Z10 period and 8-12 in the Z11-Z12 period.
7. The method of claim 4, wherein in the step (3), the added amount of the egg custard is 20% -40% of the total feeding amount, 40% -60% of the Z7-Z10 phase and 50% -75% of the Z11-Z12 phase, and the egg custard is prepared by steaming for 3-5 minutes and then sieving with a 60-80 mesh sieve.
8. The method of claim 4, wherein in the step (3), the feeding frequency of the bait is 3-4 times per day in the period Z1-Z4, 3 times per day in the period Z5, and the feeding time and the feeding amount ratio are respectively 30% in the morning of 6:00, 20% in the noon of 11:00 and 50% in the afternoon of 17:00.
9. The method for preparing the compound probiotic preparation for breeding macrobrachium rosenbergii according to claim 4, wherein the macrobrachium rosenbergii larvae are of a 'number 1' strain, and the putting density of the larvae is 10 ten thousand tails per cubic meter.
10. The use of the compound probiotic preparation for macrobrachium rosenbergii seedling culture according to any one of claims 1 to 3 for improving the survival rate of macrobrachium rosenbergii larvae, reducing the metamorphosis rate, promoting the growth and development, purifying the water quality of the seedling culture water body and/or replacing antibiotics for green healthy seedling culture.

Description

Compound probiotics preparation for breeding macrobrachium rosenbergii as well as preparation method and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a compound probiotic preparation for breeding macrobrachium rosenbergii, and a preparation method and application thereof. Background The macrobrachium rosenbergii is used as a global important freshwater economic shrimp, and the offspring seed cultivation is a key link in the cultivation industry chain and directly relates to the yield and benefit of the subsequent adult shrimp cultivation. In the intensive seedling raising process, because of high density of larvae and large feeding amount, residual baits and excrement are extremely easy to cause accumulation of harmful substances such as ammonia nitrogen, nitrite and the like in a seedling raising water body, severe environmental stress is formed on the larvae of macrobrachium rosenbergii which are not sound in an immune system, bacterial diseases such as vibriosis and the like are often induced, and finally, the industrial bottleneck of low survival rate of the larvae and uneven quality of seedlings is caused. To address these challenges, probiotic technology has been introduced into the field of aquaculture as a potential green solution. According to the technical scheme, the special microecological requirements of the macrobrachium rosenbergii in the seedling stage are met, the macrobrachium rosenbergii larvae can be optimized, differences in digestion physiological characteristics, ingestion caliber, immunity and sensitivity to water environment exist in different development stages, for example, early stages (Z1-Z4 stages), the larvae eat for the first time, intestinal flora colonization is in a critical window stage, probiotics which are easy to utilize and can occupy ecological niches rapidly are needed, the middle and later stages (Z5-P1 stages) are reached, and along with the increase of ingestion amount and the increase of metabolic waste, the conversion pressure of water ammonia nitrogen is improved, and the field still continuously needs a compound probiotic scheme capable of finely conforming to the growth rule of macrobrachium rosenbergii larvae and realizing the cooperative and accurate regulation of 'in vivo intestinal health' and 'in vitro water environment'. Disclosure of Invention The present invention has been made in view of the above-described problems occurring in the prior art. Therefore, the invention provides a compound probiotic preparation for macrobrachium rosenbergii seedling culture, which solves the problem that the prior art is difficult to synchronously meet the intestinal microecology field planting and the dynamic ammonia nitrogen conversion requirements of water bodies at different development stages of larvae. In order to solve the technical problems, the invention provides the following technical scheme: In a first aspect, the invention provides a compound probiotic preparation for culturing seedlings of macrobrachium rosenbergii, which comprises active strain components of the compound probiotic preparation, wherein the active strain components comprise lactococcus formans (Lactococcus garvieae), bacillus subtilis (Bacillus subtilis) and nitrifying bacillus vickers (Nitrobacter winogradskyi), and the preservation number of the lactococcus formans is CGMCC No.31024. As a preferable scheme of the compound probiotic preparation for culturing the seedlings of the macrobrachium rosenbergii, the compound probiotic preparation adopts different strain compositions and proportions according to different development stages of the macrobrachium rosenbergii larvae: in the stages Z1 to Z4 of the macrobrachium rosenbergii larvae, the composite probiotic preparation consists of lactococcus formans and bacillus subtilis, and the ratio of the strains is 2:1; In the stage Z5 to P1 of the macrobrachium rosenbergii larvae, the composite probiotic preparation consists of lactococcus formans, bacillus subtilis and nitrifying bacteria, and the ratio of the strains is 2:1:1. Furthermore, the strategy of the staged accurate application is characterized in that the strategy deeply meets the physiological and ecological requirements of the macrobrachium rosenbergii larvae (particularly named as 'number 1 of the number 1') in different development stages from the stage Z1 to the stage P1. In the period Z1 to Z4 (early stage of flea larvae), the egg yolk of the larvae is exhausted to start exogenous ingestion, the intestinal flora colonization is in a critical window period, and the preparation is compounded by lactococcus formans and bacillus subtilis in a ratio of 2:1, so that the beneficial bacteria are preferentially promoted to colonize and occupy the early stage of the intestinal tracts of the larvae, pathogenic bacteria are inhibited, and digestion is assisted. Entering the stage Z5 to P1 (from middle and later stages