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CN-122012278-A - Group of growth-promoting and decontamination flora and application thereof

CN122012278ACN 122012278 ACN122012278 ACN 122012278ACN-122012278-A

Abstract

The invention provides a group of growth-promoting and decontamination flora and application thereof, belonging to the technical field of phthalate polluted soil restoration. The growth-decontamination bacterial group includes Acinetobacter (Acinetobacter sp.) Sb 2-4, gordonia phthalate (Gordonia phthalatica) 107, and Bacillus somatomedii (Bacillus subtilis) 19743. Wherein the endophytic acinetobacter Sb 2-4 is preserved in China center for type culture collection (China center for type culture collection) at 12 months and 01 days of 2025, and the preservation number of the endophytic acinetobacter Sb 2-4 is CCTCC NO: M20252724. The growth promoting and dirt removing flora can realize the growth promotion of crops while ensuring the degradation effect of PAEs.

Inventors

  • GAO YANZHENG
  • CHEN JIANQIN
  • LIU XIANGYU

Assignees

  • 南京农业大学三亚研究院
  • 南京农业大学

Dates

Publication Date
20260512
Application Date
20260209

Claims (6)

  1. 1. The group of growth promoting and decontamination bacteria is characterized by comprising endophyte Acinetobacter (Acinetobacter sp.) Sb 2-4, gordon phthalate (Gordonia phthalatica) 107 and growth promoting bacillus (Bacillus subtilis) 19743, wherein the viable bacteria ratio of the endophyte Acinetobacter Sb 2-4 to the Gordon phthalate 107 to the growth promoting bacillus 19743 in the group of growth promoting and decontamination bacteria is 1-1.5:1-1.5:1-1.5, the endophyte Acinetobacter Sb 2-4 is preserved in China center for type culture collection (China center for type culture collection) in 12 months 01, and the preservation number of the endophyte Acinetobacter Sb 2-4 is CCTCC NO: M20252724.
  2. 2. Use of a growth-promoting-decontaminating flora according to claim 1 for promoting crop growth and for restoring phthalate contaminated soil.
  3. 3. The use according to claim 2, wherein the use comprises preparing a microbial inoculum from the growth-promoting and decontamination flora, inoculating the microbial inoculum into farmland soil contaminated by phthalate esters to promote crop growth and phthalate ester degradation, wherein the inoculum is inoculated in an amount of 5-10% of the farmland soil mass.
  4. 4. The use according to claim 3, wherein the bacterial agent is a bacterial suspension or an immobilized bacterial agent.
  5. 5. The use according to claim 4, wherein the method of preparing the bacterial suspension comprises: Respectively carrying out activation treatment on the endophyte Acinetobacter Sb 2-4, the Gordonia phthalate 107 and the Bacillus natto 19743 to obtain activated endophyte Acinetobacter Sb 2-4, activated Gordonia phthalate 107 and activated Bacillus natto 19743; According to the viable bacteria ratio of 1-1.5:1-1.5:1-1.5, the activated endophyte Acinetobacter Sb 2-4, the activated Gordon phthalate strain 107 and the activated Bacillus thuringiensis 19743 are mixed and prepared into a bacterial suspension with the OD 600nm value of 1.
  6. 6. The use according to claim 5, wherein the preparation method of the immobilized microbial agent comprises: pyrolyzing rice stalks or cornstalks at 800-1200 ℃ for 12-24 hours to obtain an activated carbon carrier; Adding the bacterial suspension into an active carbon carrier according to a bacterial material ratio of 20-30mL:1.5g, solidifying for 0.5-4d in an oscillation incubator, and centrifuging by using a disc centrifuge to obtain the immobilized bacterial agent.

Description

Group of growth-promoting and decontamination flora and application thereof Technical Field The invention relates to the technical field of phthalate polluted soil restoration, in particular to a group of growth-promoting and pollution-eliminating flora and application thereof. Background Phthalate esters (PAEs) are widely used in water, soil, atmosphere and other environmental media through various ways such as industrial wastewater, plastic garbage degradation, domestic sewage discharge and the like, and are detected even in remote areas such as polar regions and the like, so that the phthalate esters (PAEs) have strong migration and diffusion capacities. The environmental risk of PAEs is not only due to their ubiquitous nature, but also to their serious threat to the ecosystem and human health. In the prior art, PAEs degrading bacterial groups constructed by PAEs degrading bacterial strains are often adopted to repair phthalate polluted soil, but the existing PAEs degrading bacterial groups cannot promote crop growth while the PAEs degrading effect is not good enough. Disclosure of Invention The invention provides a group of growth-promoting and decontamination flora and application thereof, which can realize crop growth promotion while ensuring the degradation effect of PAEs. In order to achieve the above purpose, the invention adopts the following technical scheme: In a first aspect, the invention provides a group of growth-promoting and decontamination flora, wherein the growth-promoting and decontamination flora comprises endophyte acinetobacter (Acinetobacter sp.) Sb 2-4, gordon phthalate (Gordonia phthalatica) 107 and growth-promoting bacillus (Bacillus subtilis) 19743, and the viable bacteria ratio of the endophyte acinetobacter Sb 2-4, the Gordon phthalate 107 and the growth-promoting bacillus 19743 in the growth-promoting and decontamination flora is 1-1.5:1-1.5:1-1.5. The endophyte acinetobacter Sb2-4 and the Gordonia phthalate 107 are PAEs degrading bacteria, and the growth promoting bacillus 19743 is growth promoting bacteria. Acinetobacter Sb2-4 (Acinetobacter sp.Sb2-4) is preserved in China center for type culture Collection (China center for type culture collection) in year 2025 and 12, and the preservation number of the Acinetobacter Sb2-4 is CCTCC NO: M20252724. The Gordon phthalate 107 is purchased from China industry microbiological culture Collection center (CICC), the preservation number is CICC 24107, and the preservation address is Nanjing's Zhengling street 50 from Jiangsu province. Bacillus thuringiensis 19743 is purchased from China center for collection of microorganisms and strains (ACCC), the collection number is ACCC 19743, and the collection address is the resource building of national academy of agricultural resources and agricultural division research institute, guangcun south street 12 in the sea lake area of Beijing city. In one implementation of the first aspect, the 16S rRNA sequence of Acinetobacter endogenously provided by Sb 2-4 is shown in SEQ ID NO. 1. In a second aspect, the present invention provides the use of a group of growth-promoting-decontaminating flora according to the first aspect for promoting crop growth and restoring phthalate contaminated soil. In one implementation mode of the second aspect, the application comprises preparing a microbial inoculum by adopting growth promoting and pollution removing flora, inoculating the microbial inoculum into farmland soil polluted by phthalate to realize crop growth promotion and phthalate degradation, wherein the inoculation amount of the microbial inoculum is 5-10% of the mass of the farmland soil. In one implementation manner of the second aspect, the growth-promoting and decontamination bacterial agent is a bacterial suspension or an immobilized bacterial agent. In one implementation manner of the second aspect, a method for preparing a bacterial suspension includes: Respectively carrying out activation treatment on the endophyte Acinetobacter Sb 2-4, the Gordonia phthalate 107 and the Bacillus natto 19743 to obtain activated endophyte Acinetobacter Sb 2-4, activated Gordonia phthalate 107 and activated Bacillus natto 19743; According to the viable bacteria ratio of 1-1.5:1-1.5:1-1.5, the activated endophyte Acinetobacter Sb 2-4, the activated Gordon phthalate strain 107 and the activated Bacillus thuringiensis 19743 are mixed and prepared into a bacterial suspension with the OD 600nm value of 1. In one implementation manner of the second aspect, a method for preparing an immobilized microbial agent includes: pyrolyzing rice stalks or cornstalks at 800-1200 ℃ for 12-24 hours to obtain an activated carbon carrier; Adding the bacterial suspension into an active carbon carrier according to a bacterial material ratio of 20-30mL to 1.5g, solidifying for 0.5-4d in an oscillation incubator, and centrifuging by using a disc centrifuge to obtain the immobilized bacterial agent. Compared with the prior art, the inven