CN-122012279-A - Seawater culture pond environment regulation and control based on bottom nourishing and mixed microbial inoculum

CN122012279ACN 122012279 ACN122012279 ACN 122012279ACN-122012279-A

Abstract

The invention relates to a sea water stichopus japonicus culture pond environment regulation and control method based on a bottom-nourishing device and a mixed microbial inoculum and application thereof. According to the invention, the bottom-nourishing operation is carried out on the sea water stichopus japonicus culture pond, and meanwhile, the mixed microbial inoculum of the rhodobacter sphaeroides, the phosphophaga bacillus, the Altolochia and the enterococcus faecalis is combined, so that the water quality of the stichopus japonicus culture pond is improved, and meanwhile, the immunity of the stichopus japonicus is enhanced, and the invention has remarkable environmental and economic benefits.

Inventors

XIAO YAO
JIANG JINGWEI
DONG YING
ZHOU ZUNCHUN
SONG GANG
WANG BAI
GAO SHAN
WANG XUDA
LI PEIPEI
ZHAO DAQIAN
Jiang Pingzhe
YUE DONGMEI

Assignees

辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站)

Dates

Publication Date: 20260512
Application Date: 20260210

Claims (10)

1. The mixed microbial inoculum for the environment regulation of the sea water stichopus japonicus culture pond is characterized by comprising rhodobacter sphaeroides (Rhodobacter sphaeroides), phospho-phagostimulant bacillus (Fictibacillus phosphorivorans), amola almoraxella (Alteromonas abrolhosensis) and enterococcus faecalis (Enterococcus faecalis), wherein the preservation number of enterococcus faecalis is CICC 23658, the preservation number of phospho-phagostimulant bacillus is CGMCC No.36465, the preservation number of amola almoraxella is CGMCC No.36464, and the preservation number of rhodobacter sphaeroides is ATCC 17023.
2. The mixed microbial inoculant according to claim 1, wherein the concentration ratio of rhodobacter sphaeroides, bacillus phosphophaga, moraxella alpina and enterococcus faecalis in the mixed microbial inoculant is 100-10:10-1:10-1:10-1.
3. The mixed microbial agent according to claim 3, wherein the rhodobacter sphaeroides concentration is 10 8 -10 9 cfu/ml, the Bacillus sphaericus concentration is 10 7 -10 8 cfu/ml, the Moraxella albolani concentration is 10 7 -10 8 cfu/ml, and the enterococcus faecalis concentration is 10 7 -10 8 cfu/ml.
4. The mixed microbial inoculum according to any one of claims 1-3 is characterized in that the preparation steps of the mixed microbial inoculum are that the rhodobacter sphaeroides stock solution, the phosphophaga bacillus stock solution, the alto Moraxella stock solution and the enterococcus faecalis stock solution are taken, mixed, added with sterile seawater and stored at 4 ℃ for standby.
5. The environment regulation and control method for the sea water stichopus japonicus culture pond is characterized by comprising the following steps: 1) Performing bottom-nourishing operation on the stichopus japonicus culture pond; 2) And sprinkling the mixed microbial inoculum according to any one of claims 1-4 to the water body after bottom-nourishing.
6. The environment control method according to claim 5, wherein the bottom-nourishing operation is performed based on a bottom-nourishing device, the bottom-nourishing device consists of a water pumping device, an engine, a floating row, a water draining pipe, a water inlet pipe, a transverse water pipe and a bottom-nourishing pipe, the water pumping device and the engine are arranged at the upper end of the floating row, the water pumping device is connected with the water draining pipe and the water inlet pipe, the tail end of the water draining pipe is connected with the transverse water pipe, the transverse water pipe is vertically connected with the bottom-nourishing pipe, and the bottom-nourishing pipe is perpendicular to the water surface and extends to the water; The bottom-nourishing device comprises a water pumping device, an engine, a floating row, a water draining pipe, a water inlet pipe, a transverse water pipe and bottom-nourishing pipes, wherein the water pumping device and the engine are arranged at the upper end of the floating row; More preferably, the number of the bottom-nourishing pipes is 10-16, the length of the bottom-nourishing pipes is 2-4 m, and the diameter of the bottom-nourishing pipes is 1-1.5 inches.
7. The environmental conditioning method according to claim 5, wherein the concentration of the mixed microbial agent is 0.5-1 ppm, and the mixed microbial agent is sprayed 1: 1mL per cubic meter of water body.
8. The environmental conditioning method according to any one of claims 5 to 6, wherein the bottom-replenishing operation is performed by using the bottom-replenishing device according to water quality conditions; Preferably: After the ice is melted in spring, when the temperature of the seawater rises to about 10 ℃, the bottom-nourishing device is utilized to carry out comprehensive deep bottom-nourishing, the tail end of a bottom-nourishing pipe is ensured to be more than 10cm away from the bottom of the pool, preferably, the bottom-nourishing frequency is 3 to 4 times per week, the mixed microbial inoculum of 0.5 to 1 ppm is sprayed after each bottom-nourishing, and the dosage of each time is 1 to mL of the mixed microbial inoculum sprayed per cubic meter of water body; In the high-temperature period in summer, when the sea water temperature reaches above 26 ℃, shallow bottom is nourished by utilizing the bottom-nourishing device, the distance between the tail end of a bottom-nourishing pipe and the pool bottom is ensured to be more than 30cm, preferably, the bottom-nourishing frequency is that the bottom-nourishing is carried out once per day, cloudy shading is only needed, 0.5-1 ppm of the mixed microbial inoculum is sprayed after each bottom-nourishing, and the use amount of each mixed microbial inoculum is that 1.1 mL of the mixed microbial inoculum is sprayed per cubic meter of water body.
9. A bottom-nourishing device for environmental regulation of a sea water stichopus japonicus culture pond is characterized by comprising a water pumping device, an engine, a floating row, drain pipes, water inlet pipes, transverse water pipes and bottom-nourishing pipes, wherein the water pumping device and the engine are arranged at the upper end of the floating row, the water pumping device is connected with two drain pipes and one water inlet pipe, the tail ends of the two drain pipes are respectively connected with two end areas of the transverse water pipes, the transverse water pipes are horizontally arranged above the floating row and are vertically connected with a plurality of bottom-nourishing pipes, the bottom-nourishing pipes are perpendicular to the water surface and extend to the water, the number of the bottom-nourishing pipes is preferably 10-16, the length of each bottom-nourishing pipe is 2-4 meters, and the diameter of each bottom-nourishing pipe is 1-1.5 inches.
10. The bottom-nourishing device according to claim 9, wherein the bottom-nourishing device is assembled by fixing the pumping device on a floating raft with an engine, the pumping device is respectively connected to two drain pipes and one water inlet pipe, the tail ends of the two drain pipes are connected to the two ends of a transverse water pipe, and the lower ends of the transverse water pipe are connected with the corresponding bottom-nourishing pipes according to the size of a pond.

Description

Seawater culture pond environment regulation and control based on bottom nourishing and mixed microbial inoculum Technical Field The invention belongs to the technical field of microbial engineering and environmental remediation, and particularly relates to a stichopus japonicus culture pond environment regulation method based on a bottom-nourishing device and application thereof. Background With the continuous development of the mariculture industry, pond culture has become an important mode of stichopus japonicus proliferation culture. The dissolved oxygen and the temperature in the pond seawater can change along with seasons, so that harmful substances in the pond water quality environment are increased in the ice melting period and the summer high-temperature period, and stichopus japonicus diseases are easy to occur suddenly. The good pond water quality environment not only can absorb harmful substances in water, but also can improve the disease resistance of stichopus japonicus, so that the stichopus japonicus is in a relatively stable growth environment, and the method is a key technical means for ensuring the success of cultivation. However, the traditional bottom oxygenation system has high cost and high energy consumption, and has certain potential safety hazards of electricity consumption, and meanwhile, substances such as feces and aquatic plant carcasses generated by stichopus japonicus are deposited at the bottom of the pond through the processes of adsorption, complexation, sedimentation and the like, so that the pond bottom is too hard, dissolved oxygen is insufficient, harmful substances such as nitrite, ammonia nitrogen, sulfide and the like are not easy to decompose, and disease burst such as stichopus japonicus skin formation and the like is easy to cause. Therefore, improving the micro-ecological environment of the seawater culture pond is a necessary requirement for the healthy development of the stichopus japonicus pond culture, and is an effective means for guaranteeing the quality safety of the aquatic products. In view of this, the present invention has been proposed. Disclosure of Invention In order to solve the technical problems in the prior art, the invention seeks a new thought of stichopus japonicus culture pond environment regulation technology, and accordingly the invention is provided. The invention at least comprises the following three purposes: 1) Seeking a mixed microbial inoculum for regulating and controlling the environment of a sea water stichopus japonicus culture pond; 2) Seeking a bottom-nourishing device for regulating and controlling the environment of a sea water stichopus japonicus culture pond; 3) A method for regulating and controlling the environment of a sea water stichopus japonicus culture pond is sought. In order to achieve the above purpose, the invention specifically provides the following technical scheme: The invention firstly provides a mixed microbial inoculum for regulating and controlling the environment of a sea water stichopus japonicus culture pond, which comprises rhodobacter sphaeroides (Rhodobacter sphaeroides), phospho-phaga bacillus (Fictibacillus phosphorivorans), altolla (Alteromonas abrolhosensis) and enterococcus faecalis (Enterococcus faecalis). Furthermore, the enterococcus faecalis has a preservation number of CICC 23658, the phosphophaga bacillus has a preservation number of CGMCC No.36465, the Moraxella alpina has a preservation number of CGMCC No.36464, and the rhodobacter sphaeroides has a preservation number of ATCC 17023. Further, the concentration ratio of rhodobacter sphaeroides, bacillus phosphophaga, moraxella alpina and enterococcus faecalis in the mixed microbial agent is 100-10:10-1:10-1:10-1. Preferably, the rhodobacter sphaeroides concentration in the mixed bacterial agent is 10 7-109 cfu/ml, the phosphophaga bacillus concentration is 10 6-108 cfu/ml, the amola algovora concentration is 10 6-108 cfu/ml, the enterococcus faecalis concentration is 10 6-108 cfu/ml, more preferably, the rhodobacter sphaeroides concentration in the mixed bacterial agent is 10 8-109 cfu/ml, the phosphophaga bacillus concentration is 10 7-108 cfu/ml, the amola algovora concentration is 10 7-108 cfu/ml and the enterococcus faecalis concentration is 10 7-108 cfu/ml. In some aspects, the specific preparation steps of the mixed microbial inoculum comprise the steps of taking rhodobacter sphaeroides stock solution with the concentration of 10 8 -109 cfu/ml, lactobacillus sakei with the concentration of 10 7 -108 cfu/ml, moraxella albolabris with the concentration of 10 7-108 cfu/ml and enterococcus faecalis stock solution with the concentration of 10 7-108 cfu/ml, mixing the above materials, adding sterile seawater, and preserving at 4 ℃ for standby. In some embodiments, the rhodobacter sphaeroides is prepared by: Fully dissolving rhodobacter sphaeroides freeze-dried powder microbial inoculum in sterile distilled water, streaking and activati