CN-122012282-A - Intestinal bacteria for improving semen quality and application thereof

Abstract

The invention discloses an intestinal tract bacterium for improving semen quality and application thereof, and belongs to the fields of microbial technology and biological medicine. Aiming at the problem of the lack of the prior art of improving high fat diet induced male reproductive lesions with specific probiotics, the present invention proposes to use the xenorhabdus strain Allobaculum stercoricanis (A.s) or an inactivated form thereof (P-A.s) for this purpose. Animal experiments show that compared with a high-fat diet model group, the supplement A.s or P-A.s can obviously inhibit abnormal weight increase, improve testis index, improve sperm density, activity, movement speed and testosterone level of testes, and can effectively repair tissue morphological damage of testes and epididymis and ultrastructural abnormality of sperms. Wherein, the improvement effect of the inactivated bacteria form P-A.s is particularly remarkable.

Inventors

• CAI RUI
• CHEN ZHONGYAO
• PANG WEIJUN
• WANG FANG
• WANG MINGYU
• HAN HAOZHE
• Lai Zhenheng

Assignees

• 西北农林科技大学

Dates

Publication Date

20260512

Application Date

20260312

Claims (8)

1. 1. A probiotic preparation for improving male reproductive lesions, characterized in that the active ingredient of said preparation comprises a strain Allobaculum stercoricanis of the genus bacillus (A.s) or an inactivated form of this strain (P-A.s), and pharmaceutically acceptable excipients.
2. 2. The probiotic preparation according to claim 1, characterized in that the daily effective dose of A.s strain or P-A.s in the preparation is not less than 1 x 10 9 CFU or equivalent of thalli.
3. 3. The probiotic preparation according to claim 1 or 2, characterized in that the preparation is in the form of a probiotic preparation, a functional food or a dietary supplement.
4. 4. Use of a strain Allobaculum stercoricanis of the genus isopobacterium (A.s) or an inactivated form thereof (P-A.s) for the preparation of a product for ameliorating male reproductive lesions.
5. 5. The use according to claim 4, wherein the male reproductive lesions are induced by a high fat diet.
6. 6. The use according to claim 4 or 5, wherein said ameliorating male reproductive lesions comprises at least one of inhibiting abnormal weight gain caused by a high fat diet, increasing testis index, increasing sperm quality, increasing testosterone relative content in testis tissue, repairing tissue morphological lesions of the seminiferous tubules and epididymis ducts, repairing lesions of the acrosome, mitochondrial sheath ultrastructure.
7. 7. The use of claim 6, wherein said improving sperm quality comprises improving at least one of sperm density, motility, curvilinear movement velocity, and linear movement velocity.
8. 8. The use according to claim 4, wherein the product is a probiotic preparation, a functional food, a dietary supplement or a feed additive.

Description

Intestinal bacteria for improving semen quality and application thereof Technical Field The invention relates to the fields of microbiological technology and biological medicine, in particular to a specific intestinal strain, i.e. a strain Allobaculum stercoricanis of the genus Isobacterium and application of an inactivated form thereof in improving male reproductive injury, and a probiotic preparation containing the strain. Background The disclosure of this background section is only intended to increase some understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art. Improving body metabolism by using intestinal strains is a research front in the current biomedical field, and related researches are gradually deepened to causal verification of specific strain-action mechanism-metabolism efficacy and product development stage from the observation of the relevance of obesity and intestinal flora. The genus isopibacterium has been shown to play a positive role in diseases such as obesity, hypertension, arthritis, etc., as a potential probiotic, of which Allobaculum stercoricanis (hereinafter abbreviated as A.s) is a core strain of interest in this genus. The strain is successfully separated in 2004, and researches show that the strain has higher abundance in healthy and slim animals, and the abundance of the strain can be obviously increased after diet-induced obese animals lose weight, which indicates that the strain is closely related to the healthy metabolic state of organisms. Currently, the studies of the genus isopibacterium (allobacilum), in particular strain A.s, are mainly focused on the field of intestinal microbiome. This bacterium is identified as an important short chain fatty acid producer in the mammalian gut and is of particular interest in the production of butyric acid. Butyric acid has a core function of anti-inflammatory, maintenance of intestinal barrier and regulation of immunity, so that in many studies an increase in the abundance of allobaculom is often associated with an improved metabolic state, for example in animal models of obesity, type 2 diabetes and inflammatory bowel disease, an increase in its abundance often being indicative of an improvement in health indicators. However, most of the existing researches are in a correlation observation level, the specific action mechanism of the bacteria and the causal role of the bacteria in host physiology are still unknown, and most of the research is limited to intestinal environments and is not extended to other physiological systems. However, the effect of A.s on the research of semen quality and male reproductive health belongs to an undeveloped blank field. At present, there is no direct evidence or published studies discussing the association between this strain and semen parameters such as sperm concentration, viability, morphology or DNA integrity. Behind this gap is the system where traditional studies treat intestinal microorganisms as relatively independent of reproductive health. However, with the rise of the concept of the "intestinal-testicular axis", the scientific community has begun to focus on the fact that intestinal flora may remotely influence testicular function and spermatogenesis through pathways such as immunomodulation, metabolite secretion and endocrine disruption. However, until now, no report has been made on whether Allobaculum stercoricanis strain or its inactivated form can be directly used for improving, especially repairing, damage to male reproductive system caused by metabolic stress such as high-fat diet, and there is no technical suggestion for developing it as a related product. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a specific intestinal strain, namely a strain Allobaculum stercoricanis of the genus Bacillus and application of an inactivated form thereof in improving male reproductive injury, and a probiotic preparation containing the strain. The technical scheme adopted by the invention is as follows: In a first aspect, the present invention provides a probiotic formulation for use in ameliorating male reproductive lesions. The active ingredient of the preparation comprises a strain Allobaculum stercoricanis (A.s) of the genus Isobacterium or an inactivated form (P-A.s) of the strain, and pharmaceutically acceptable adjuvants (such as probiotic protectant, carrier, excipient, etc.). Preferably, the daily effective dose of A.s strain or P-A.s in the formulation is not less than 1X 10 9 CFU or equivalent of cells. The preparation can be made into probiotic preparation, functional food or dietary supplement. In a second aspect, the invention provides the use of a strain Allobaculum stercoricanis (A.s) of the genus xenorhabdus or an inactivated form thereof (P-A.s) in the manufacture