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CN-122012284-A - Synthetic flora with plant growth promoting effect and application thereof

CN122012284ACN 122012284 ACN122012284 ACN 122012284ACN-122012284-A

Abstract

The invention relates to the technical field of microorganisms, in particular to a synthetic flora with a plant growth promoting effect and application thereof. The trichomonas treponini RB13 (with the preservation number of CGMCC No. 37455) and the pseudomonas 5asv2 (with the preservation number of CGMCC No. 37454) in the synthetic flora are indigenous microorganisms, so that the method is safe and pollution-free to the environment, does not generate any harmful residues in the application process, and does not destroy the balance of the original soil microbial community. Meanwhile, due to the good adaptability to low-temperature adversity and high growth promotion efficiency, the method can be widely applied to agricultural production in alpine regions, and solves the problems of slow growth and low yield of crops in the alpine regions. The invention provides a new technical approach for agricultural sustainable development of Qinghai-Tibet plateau and even other high-cold high-altitude areas, and has important significance for guaranteeing the safety of highland grains and ecological environment.

Inventors

  • CHU HAIYAN
  • CHEN SIYU
  • Gao Ruikun
  • ZHANG YUXIAO
  • LV LIHUI

Assignees

  • 中国科学院南京土壤研究所

Dates

Publication Date
20260512
Application Date
20260316

Claims (10)

  1. 1. The synthetic flora with the effect of promoting plant growth is characterized by comprising trichococcus equisimilis (Trichococcus sp.) RB13 and Pseudomonas sp 5asv2, wherein the collection number of the trichococcus equisimilis RB13 is CGMCC No.37455, and the collection number of the Pseudomonas sp 5asv2 is CGMCC No.37454.
  2. 2. The synthetic flora according to claim 1, wherein the ratio of viable count of trichomonas bundle RB13 to pseudomonas 5asv2 in the synthetic flora is 0.5-2:1.
  3. 3. A composite microbial agent, characterized in that the active ingredient comprises the synthetic flora according to claim 1 or 2.
  4. 4. The composite microbial agent according to claim 3, wherein the composite microbial agent comprises a liquid preparation or a solid preparation, the viable count of the composite microbial agent is not less than 1X 10 8 CFU/ml when the composite microbial agent is a liquid preparation, and the viable count of the composite microbial agent is not less than 1X 10 8 CFU/g when the composite microbial agent is a solid preparation.
  5. 5. The method for preparing the composite microbial inoculum according to claim 3 or 4, which is characterized by comprising the following steps: After respectively activating the trichomonas campestris RB13 and the pseudomonas 5asv2, mixing and inoculating the trichomonas campestris RB13 and the pseudomonas 5asv2 into a culture medium to be cultured to a logarithmic phase, wherein the culture medium comprises a trypticase liquid culture medium.
  6. 6. The method according to claim 5, wherein the temperature of the culture is 25-30 ℃.
  7. 7. Use of the synthetic flora according to claim 1 or 2 or the complex microbial inoculant according to claim 3 or 4 or prepared by the preparation method according to claim 5 or 6 in one or more of claims 1) -3): 1) Promoting plant growth, wherein the plant comprises highland barley; 2) Improving soil fertility; 3) The rhizosphere soil enzyme activity is improved, and the rhizosphere soil enzyme comprises one or more of urease, alkaline phosphatase, alkaline protease, beta-glucosidase, alpha-glucosidase and beta-galactosidase.
  8. 8. The use according to claim 7, wherein the promotion of plant growth comprises promotion of plant growth in a low temperature environment having a temperature in the range of 10 ℃ to 20 ℃.
  9. 9. A method for promoting plant growth, which comprises applying the synthetic flora according to claim 1 or 2 or the composite microbial inoculum according to claim 3 or 4 or the composite microbial inoculum prepared by the preparation method according to claim 5 or 6 to the rhizosphere area of plants.
  10. 10. The method of claim 9, wherein the viable count of the synthetic flora in the rhizosphere area of the plant after application is greater than or equal to 1 x 10 8 CFU/g soil.

Description

Synthetic flora with plant growth promoting effect and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a synthetic flora with a plant growth promoting effect and application thereof. Background Wetland is one of three ecological systems, is critical to the survival and development of human beings, provides necessary biodiversity and ecological system services, and plays a key role in hydrologic regulation, bio-geochemical circulation and biodiversity protection. The Qinghai-Tibet plateau is called the earth's "third pole" and "Asian water tower", and its ecological environment is highly sensitive to global climate change and is a magnifying glass for global climate change. The Qinghai-Tibet plateau alpine-cold wetland is inoculated with various and unique microorganism resources due to the unique extreme environments such as low temperature, strong radiation, drought, high salt and alkali, and the like, and is regarded as a 'repository' of microorganism resources in various ancient extreme environments. These microorganisms have unique metabolic characteristics and extremely high scientific research value. At present, the excavation and utilization of soil microbial strain resources of typical wetland in Qinghai-Tibet plateau are still limited. Decoding functional genes of the microbial strains, revealing genetic characteristics of flora, establishing a system of soil microbial germplasm resource library of Qinghai-Tibet plateau, helping to protect microbial diversity in climate sensitive areas, and providing theoretical basis for exploring special features, adaptation mechanism and evolution history of life under extreme environmental conditions. Highland barley (highland barley) is one of the main food crops in Qinghai-Tibet plateau, and grows in the environment of high cold and anoxia and soil impoverishment throughout the year. For a long time, the local agricultural production is faced with the problems of slow crop growth, low soil nutrient availability and the like caused by high and cold low temperature. Although some growth promoting bactericides are used for improving crop yield at present, most of the growth promoting bactericides are derived from microorganisms in normal temperature areas of plain, and often have insufficient activity and unsatisfactory effect in extreme environments of low temperature and high altitude in Qinghai-Tibet plateau. Therefore, screening of indigenous microorganisms from the special environment of Qinghai-Tibet plateau is urgently needed to construct functional flora which is suitable for the alpine environment and has remarkable growth promoting effect, and is used for improving the growth and yield of crops such as highland barley and the like. Disclosure of Invention In order to solve the problems, the invention provides a synthetic flora with plant growth promoting effect and application thereof. The synthetic flora provided by the invention can stably colonize the rhizosphere of highland barley at a low temperature (10-20 ℃), and the turnover and supply capacity of nutrients of the rhizosphere are enhanced, so that the root system development of the highland barley is promoted and the plant growth is driven. In order to achieve the above object, the present invention provides the following technical solutions: the invention provides a synthetic flora with a plant growth promoting effect, which comprises trichococcus (Trichococcus) RB13 and pseudomonas (Pseudomonas sp.) 5asv2, wherein the collection number of the trichococcus RB13 is CGMCC No.37455, and the collection number of the pseudomonas 5asv2 is CGMCC No.37454. Preferably, the ratio of the viable count of the trichomonas bundle RB13 to the viable count of the pseudomonas 5asv2 in the synthetic flora is 0.5-2:1. The invention provides a composite microbial inoculum, and the active ingredients comprise the synthetic flora in the technical scheme. Preferably, the dosage form of the composite microbial inoculum comprises a liquid preparation or a solid preparation, when the dosage form of the composite microbial inoculum is the liquid preparation, the viable count of the composite microbial inoculum is more than or equal to 1 multiplied by 10 8 CFU/ml, and when the dosage form of the composite microbial inoculum is the solid preparation, the viable count of the composite microbial inoculum is more than or equal to 1 multiplied by 10 8 CFU/g. The invention provides a preparation method of the composite microbial inoculum, which comprises the following steps: after respectively activating the trichomonas campestris RB13 and the pseudomonas 5asv2, inoculating the trichomonas campestris RB13 and the pseudomonas 5asv2 into a culture medium for culturing to a logarithmic growth phase, wherein the culture medium comprises a trypticase liquid culture medium. Preferably, the temperature of the culture is 25-30 ℃. The invention provides the application