CN-122012286-A - Composite probiotic composition and application thereof in products for improving intestinal health

Abstract

The application provides a composite probiotic composition and application thereof in a product for improving intestinal health, wherein the composite probiotic composition comprises lactobacillus rhamnosus GG, lactobacillus compound powder, lactobacillus plantarum GOLDGUT-LP1024 and lactobacillus paracasei GOLDGUT-LC12345, the lactobacillus compound powder is formed by mixing bifidobacterium longum subspecies BORI, lactobacillus acidophilus AD031, bifidobacterium bifidum BGN4, bifidobacterium animalis subspecies AD011 and bifidobacterium longum subspecies infantis IBS007, and the lactobacillus plantarum GOLDGUT-LP1024 is preserved in the China general microbiological culture collection center with the preservation number of CGMCC No.28801. The composite probiotic composition provided by the application can promote intestinal immunity and improve intestinal metabolism so as to improve intestinal health and relieve antibiotic-associated diarrhea.

Inventors

• ZHANG SILU
• ZHOU XIAOLI
• ZHONG SHUYING
• XIAO GUOXUN

Assignees

• 深圳保时健生物工程有限公司

Dates

Publication Date

20260512

Application Date

20260326

Claims (10)

1. 1. The composite probiotic composition and application thereof in products for improving intestinal health are characterized in that the composite probiotic composition comprises lactobacillus rhamnosus GG, lactobacillus compound powder, lactobacillus plantarum GOLDGUT-LP1024 and lactobacillus paracasei GOLDGUT-LC12345, wherein the lactobacillus compound powder is formed by mixing bifidobacterium longum subspecies BORI, lactobacillus acidophilus AD031, bifidobacterium bifidum BGN4, bifidobacterium animalis subspecies AD011 and bifidobacterium longum subspecies infantis IBS 007; The lactobacillus plantarum GOLDGUT-LP1024 is preserved in China general microbiological culture Collection center (CGMCC No. 28801) with a preservation address of China academy of sciences of China, no. 3, north Star, the Korean area of Beijing city.
2. 2. The composite probiotic composition according to claim 1, characterized in that the mass ratio of lactobacillus rhamnosus GG, lactobacillus compound powder, lactobacillus plantarum GOLDGUT-LP1024 and lactobacillus paracasei GOLDGUT-LC12345 is (2-3): 6-7): 10-11): 7-8.
3. 3. The composite probiotic composition according to claim 1, characterized in that the total viable count specification of the composite probiotic composition is not less than 2.5 x 10 11 CFU/g.
4. 4. A complex probiotic preparation, characterized in that it comprises a complex probiotic composition according to any one of claims 1 to 3, and comprises pharmaceutically or food acceptable excipients.
5. 5. The compound probiotic preparation according to claim 4, wherein the preparation is in the form of solution, powder, capsule, tablet or granule, and the auxiliary material comprises any one or a combination of at least two of protective agent, filler, adhesive, emulsifying agent, solvent, colorant, pH regulator, bacteriostatic agent or buffering agent.
6. 6. The composite probiotic preparation according to claim 5, characterized in that it is a powder, prepared by the following method: Mixing lactobacillus rhamnosus GG bacterial powder, lactobacillus compound powder, lactobacillus plantarum GOLDGUT-LP1024 bacterial powder and lactobacillus paracasei GOLDGUT-LC12345 bacterial powder according to mass ratio to obtain the compound probiotic preparation, wherein the lactobacillus compound powder is formed by mixing bifidobacterium longum subspecies BORI bacterial powder, lactobacillus acidophilus AD031 bacterial powder, bifidobacterium bifidum BGN4 bacterial powder, bifidobacterium animalis subspecies AD011 bacterial powder and bifidobacterium longum subspecies infant IBS007 bacterial powder, wherein: The lactobacillus plantarum GOLDGUT-LP1024 bacterial powder is freeze-dried powder obtained by fermenting and centrifuging lactobacillus plantarum GOLDGUT-LP1024 bacterial strains through an MRS culture medium and then freeze-drying bacterial sludge.
7. 7. The use according to claim 1, wherein the improvement of intestinal health comprises an increase in intestinal immune function and an increase in intestinal metabolic function.
8. 8. The use of claim 7, wherein the enhancing intestinal immune function comprises at least one of: increasing the expression level of zonulin ZO-1 and mucin MUC2 in the colon; Modulating the gene expression levels of colonic genes Tjp, muc2, ocln, cldn1, gja, SCN9A, and inflammatory factors Il6, il 10; Increasing the expression level of colonic anti-inflammatory factors IL-4 and IL-10; Reduces the expression level of IL-1 beta of the colon pro-inflammatory factor.
9. 9. The use of claim 7, wherein the enhancing intestinal metabolic function comprises at least one of: reducing the expression level of urea nitrogen BUN in serum; improving the expression level of intestinal acetic acid and propionic acid; reducing the content of short chain fatty acid in intestinal tracts; improving the diversity of strains in intestinal tracts; Promoting the growth of probiotics in the intestinal flora and inhibiting the growth of pathogenic bacteria in the intestinal flora; and the key pathway of short chain fatty acid metabolism is improved.
10. 10. The use according to any one of claims 7 to 9, wherein the product comprises a pharmaceutical, nutraceutical or food product, and wherein the product has the effect of alleviating the symptoms of intestinal discomfort, including antibiotic-associated diarrhea, by improving the health of the intestinal tract.

Description

Composite probiotic composition and application thereof in products for improving intestinal health Technical Field The application belongs to the technical field of microorganisms, and particularly relates to a composite probiotic composition and application thereof in products for improving intestinal health. Background The intestinal tract is an important digestive organ and immune barrier organ of a human body, and the maintenance of the microecological balance of the intestinal tract is a core foundation of human health. The intestinal flora is used as a core composition of intestinal microecology, the stability of the structure and the function of the intestinal flora directly influences the integrity of intestinal mucosa barriers, the immunoregulation function and the metabolic homeostasis, once the intestinal flora is unbalanced, the problems of damaged intestinal barriers, immune disorder, metabolic abnormality and the like are easily caused, and then intestinal discomfort symptoms such as diarrhea, abdominal distention, intestinal inflammation and the like are induced, and the systemic immunity and the metabolic function are also influenced when the intestinal flora is severe, so that the intestinal microecology becomes the cause of various intestinal related diseases. Probiotics are important means for improving intestinal health as core active substances for regulating intestinal microecological balance. The probiotics can maintain intestinal microecological steady state by regulating intestinal flora structure, enhancing intestinal mucosa barrier function, regulating intestinal immunity, etc. However, when the traditional single strain probiotics are used for regulating intestinal microecology, the problems of limited strain suitability, single function, difficulty in adapting to different intestinal environments and the like exist, and the diversified intestinal health regulation requirements are difficult to meet. Along with the deep research of intestinal health, the diversity and stability of intestinal flora are gradually discovered to be the key for maintaining intestinal homeostasis, and a single probiotic strain is difficult to cover the full-dimension requirement of intestinal microecological regulation. Meanwhile, the intestinal health problem is not only limited to the local part of the intestinal canal, but also closely related to the systemic immunity, metabolism and other systems, and the comprehensive improvement of the intestinal canal steady state of the single-function probiotic preparation is difficult to realize. Therefore, there is a need in the field of intestinal health for a complex probiotic composition that effectively synergistically regulates intestinal flora structure, enhances intestinal barrier function, regulates intestinal immunity and metabolism. Disclosure of Invention The application provides a composite probiotic composition and application thereof in products for improving intestinal health, and by means of the synergistic effect of a plurality of strains of screened probiotics, the intestinal mucosa barrier is repaired, the intestinal flora structure and the metabolic function are regulated, so that a high-efficiency and safe technical scheme is provided for intestinal health management, intestinal discomfort can be effectively relieved, the intestinal barrier function is improved, and the stable reconstruction of intestinal tracts and the improvement of related health problems are assisted. In a first aspect, the embodiment of the application provides a composite probiotic composition and application thereof in a product for improving intestinal health, wherein the composite probiotic composition comprises lactobacillus rhamnosus GG, lactobacillus compound powder, lactobacillus plantarum GOLDGUT-LP1024 and lactobacillus paracasei GOLDGUT-LC12345, and the lactobacillus compound powder is formed by mixing bifidobacterium longum BORI, lactobacillus acidophilus AD031, bifidobacterium bifidum BGN4, bifidobacterium animalis milk subspecies AD011 and bifidobacterium longum infant subspecies IBS 007; The lactobacillus plantarum GOLDGUT-LP1024 is preserved in China general microbiological culture Collection center (CGMCC No. 28801) with a preservation address of China academy of sciences of China, no. 3, north Star, the Korean area of Beijing city. In some embodiments, the mass ratio of lactobacillus rhamnosus GG, lactobacillus compound powder, lactobacillus plantarum GOLDGUT-LP1024 and lactobacillus paracasei GOLDGUT-LC12345 is (2-3): 6-7): 10-11): 7-8. In some embodiments, the total viable count specification of the composite probiotic composition is ≡2.5X10 11 CFU/g. In a second aspect, embodiments of the present application provide a composite probiotic preparation comprising a composite probiotic composition according to the first aspect, and pharmaceutically or food acceptable excipients. In some embodiments, the formulation is in the form of a solution, pow