Search

CN-122012287-A - Synthetic biocontrol flora for preventing and controlling occurrence of wheat stem basal rot and application thereof

CN122012287ACN 122012287 ACN122012287 ACN 122012287ACN-122012287-A

Abstract

The invention discloses a synthetic biocontrol flora for preventing and controlling the occurrence of wheat stem basal rot and application thereof, and relates to the technical field of microorganisms. The synthetic biocontrol flora comprises streptomyces brown (Streptomyces aquilus) L69 and pseudomonas korea (Pseudomonas koreensis) S25, wherein the preservation numbers of the streptomyces brown L69 and the pseudomonas korea S25 are GDMCC No:67852 and GDMCC No:67851 respectively. After the synthetic biocontrol flora provided by the invention is applied to plants, the colonization level of fusarium pseudograminearum in the rhizosphere and the root system of wheat can be effectively reduced, and the accumulation of wheat stem basal rot germs in the bodies of the plants can be reduced, so that the prevention and control of the wheat stem basal rot can be realized. The invention provides a new technical path for preventing and controlling the wheat stem rot soil-borne diseases, and has good popularization and application prospects.

Inventors

  • WU JIALING
  • WU CHUANFA
  • GE TIDA
  • LI JIAYANG
  • GUO JUN

Assignees

  • 宁波大学

Dates

Publication Date
20260512
Application Date
20260401

Claims (10)

  1. 1. A synthetic biocontrol flora for controlling the occurrence of wheat stem basal rot, comprising streptomyces brown (Streptomyces aquilus) L69 and pseudomonas korea (Pseudomonas koreensis) S25; the deposit numbers of the brown streptomycete L69 and the Korean Pseudomonas S25 are GDMCC No:67852 and GDMCC No:67851, respectively.
  2. 2. Streptomyces brown L69 for preventing and controlling the occurrence of wheat stem rot, wherein the preservation number of the streptomyces brown L69 is GDMCC No:67852.
  3. 3. A korean pseudomonas S25 for controlling the occurrence of stalk rot of wheat, wherein the korean pseudomonas S25 has a deposit number of GDMCC No:67851.
  4. 4. Use of a synthetic biocontrol flora according to claim 1 for the preparation of a microbial agent for controlling the occurrence of wheat stem basal rot.
  5. 5. A composite microbial preparation for controlling the occurrence of wheat stem basal rot, comprising the synthetic biocontrol flora of claim 1.
  6. 6. Use of streptomyces brown color L69 according to claim 2 or pseudomonas koraiensis S25 according to claim 3 for preparing a microbial preparation for controlling the occurrence of wheat stem basal rot.
  7. 7. A microbial preparation for controlling the occurrence of wheat stem basal rot, characterized by comprising streptomyces brown strain L69 according to claim 2 or pseudomonas koraiensis S25 according to claim 3.
  8. 8. Use of the synthetic biocontrol flora according to claim 1, the streptomyces brown set forth in claim 2, L69 or the pseudomonas korea S25 according to claim 3 for controlling the occurrence of wheat stem basal rot.
  9. 9. Use of the compound microbial preparation according to claim 5 or the microbial preparation according to claim 7 for controlling the occurrence of wheat stem basal rot.
  10. 10. A method for controlling the occurrence of wheat stem rot, comprising the step of applying the synthetic biocontrol flora according to claim 1, the streptomyces brown strain L69 according to claim 2 or the pseudomonas koraiensis S25 according to claim 3 to wheat plants.

Description

Synthetic biocontrol flora for preventing and controlling occurrence of wheat stem basal rot and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a synthetic biocontrol flora for preventing and controlling the occurrence of wheat stem basal rot and application thereof. Background In recent years, wheat stem-based rot caused by fusarium pseudograminearum (Fusarium pseudograminearum) continuously occurs in a plurality of main producing areas and tends to be aggravated, and the wheat stem-based rot has become one of important soil-borne diseases threatening stable production of wheat. The pathogenic bacteria mainly infects the vascular tissue at the stem base of the plant, which causes the symptoms of premature senility, wilting, white spike and the like of the plant, and seriously affects the formation of seeds and the final yield. The existing prevention and control measures mainly depend on disease-resistant variety breeding, agricultural cultivation regulation and control and limited chemical agent application. However, factors such as lack of resistance resources, insufficient resistance stability, complex and changeable field ecological environment and the like limit the long-term prevention and control effects of the measures. Meanwhile, chemical control is limited in control effect, and ecological risks such as pesticide residue, pathogen resistance enhancement and soil microecology unbalance are possibly brought, so that a new approach for preventing and controlling diseases, which is more green, safer and sustainable, is urgently needed to be explored. The rhizosphere microbiome is taken as an important functional group for the interaction of plants and soil environment, is regarded as a second genome for the healthy regulation of crops, and plays a key barrier role in inhibiting infection of soil-borne pathogens, maintaining ecological balance of rhizosphere, promoting the growth of crops and the like. The beneficial microorganisms are utilized to regulate and control the rhizosphere microecological environment and improve the stress resistance of plants, and the method is considered as a green and sustainable disease prevention and control strategy. However, the existing biocontrol agents are mostly applied in the form of single strain, the field environmental adaptability and the control stability are insufficient, and the synergistic requirements of disease inhibition and crop growth quality improvement are difficult to be met. Therefore, the development of the synthetic biocontrol flora which can stably colonize under field conditions, has the functions of biocontrol and growth promotion and is suitable for preventing and controlling the wheat stem-based rot has important significance for improving the healthy production level of wheat and promoting the green sustainable development of agriculture. Disclosure of Invention The invention aims to provide a synthetic biocontrol flora for preventing and controlling the occurrence of wheat stem basal rot and application thereof, so as to solve the problems in the prior art. After the synthetic biocontrol flora is applied to plants, the colonization level of fusarium pseudograminearum in the rhizosphere and root systems of wheat can be effectively reduced, and the accumulation of wheat stem basal rot germs in plants is reduced, so that the prevention and control of the wheat stem basal rot are realized. In order to achieve the above object, the present invention provides the following solutions: The invention provides a synthetic biocontrol flora for preventing and controlling the occurrence of wheat stem basal rot, which comprises streptomyces brown (Streptomyces aquilus) L69 and pseudomonas koraiensis (Pseudomonas koreensis) S25; the deposit numbers of the brown streptomycete L69 and the Korean Pseudomonas S25 are GDMCC No:67852 and GDMCC No:67851, respectively. The invention also provides the streptomyces brown which is used for preventing and controlling the occurrence of the wheat stem-based rot, wherein the preservation number of the streptomyces brown L69 is GDMCC No:67852. The invention also provides a Korean pseudomonas S25 for preventing and controlling the occurrence of the wheat stem basal rot, wherein the preservation number of the Korean pseudomonas S25 is GDMCC No:67851. The invention also provides application of the synthetic biocontrol flora in preparing a microbial preparation for preventing and controlling the occurrence of wheat stem basal rot. The invention also provides a compound microbial preparation for preventing and controlling the occurrence of the wheat stem basal rot, which comprises the synthetic biocontrol flora. The invention also provides an application of the streptomyces brown strain L69 or the pseudomonas Korean S25 in preparing a microbial preparation for preventing and controlling the occurrence of the wheat stem rot. The invention also provides a