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CN-122012288-A - Acid-resistant nitrogen fixation-activated ferric phosphate synthetic flora, composite microbial inoculum and application thereof

CN122012288ACN 122012288 ACN122012288 ACN 122012288ACN-122012288-A

Abstract

The invention relates to the technical field of microorganisms, in particular to an acid-resistant nitrogen fixation-activated ferric phosphate synthetic flora and application thereof, which consists of microbial strains, wherein the preservation number of the pantoea agglomerans P-23 is CGMCC No.37622, and the preservation number of the pseudomonas donghuensis N-13 is CGMCC No.37624, so that the acid-resistant nitrogen fixation-activated ferric phosphate synthetic flora is suitable for synchronous biological reinforcement of nitrogen and phosphorus nutrients in an acid oligotrophic environment.

Inventors

  • DAI ZHONGMIN
  • Cheng Shuxun
  • XU JIANMING

Assignees

  • 浙江大学

Dates

Publication Date
20260512
Application Date
20260407

Claims (7)

  1. 1. The acid-resistant nitrogen fixation-activated ferric phosphate synthetic flora is characterized by comprising microbial strains of Pseudomonas eastern lake (Pseudomonas donghuensis) N-13 and Pantoea agglomerans (Pantoea agglomerans) P-23, wherein the preservation number of the Pantoea agglomerans P-23 is CGMCC No.37622, and the preservation number of the Pseudomonas eastern lake N-13 is CGMCC No.37624.
  2. 2. The synthetic flora of claim 1, wherein the ratio of the number of viable bacteria effective for pantoea agglomerans P-23 to pseudomonas eastern lake N-13 is 1:1.
  3. 3. A composite microbial agent comprising the synthetic flora of claim 1 or 2.
  4. 4. The composite microbial inoculant according to claim 3, wherein the OD 600 of the synthetic flora in the composite microbial inoculant is greater than or equal to 0.5.
  5. 5. The application of the synthetic flora according to claim 1 or 2 or the composite microbial inoculum according to claim 3 or 4 in biological nitrogen fixation and indissolvable phosphate activation and phosphorus release under acidic conditions, wherein the acidic conditions comprise acidic soil or acidic water, the pH range of the acidic conditions is 3.0-7.0, and the indissolvable phosphate is ferric phosphate or aluminum phosphate.
  6. 6. A method for synchronously increasing the content of available nitrogen and available phosphorus in an acid oligotrophic environment, which is characterized in that the synthetic flora according to claim 1 or 2 or the composite microbial agent according to claim 3 or 4 is applied to the acid oligotrophic environment.
  7. 7. The method of claim 6, wherein the acidic oligotrophic environment is an acidic soil or acidic water body having a poorly soluble phosphate as the sole or primary phosphorus source, and wherein the poorly soluble phosphate is ferric phosphate or aluminum phosphate.

Description

Acid-resistant nitrogen fixation-activated ferric phosphate synthetic flora, composite microbial inoculum and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to an acid-resistant nitrogen fixation-activated ferric phosphate synthetic flora and application thereof. Background In a variety of acidic environments, including partially acidified black soil, the lack of available nitrogen and available phosphorus in the soil is a key factor limiting ecological restoration and agricultural production. Acid stress not only leads phosphorus to be fixed by iron and aluminum to form indissolvable phosphate (such as ferric phosphate), but also obviously inhibits the activity of nitrogen-fixing microorganisms, thus causing nutrient double starvation. Currently, nutrient improvement for acidic environments mainly depends on lime application to adjust the pH or large investment of chemical nitrogen and phosphorus fertilizers. Although the methods can take effect in a short period, the cost is high, and secondary environmental problems such as soil hardening, salinization, water eutrophication and the like are easily caused. Bioremediation pathways, particularly with functional microorganisms, are green sustainable repair pathways, and are considered an environmentally friendly and sustainable alternative strategy. However, the existing functional bacterial agents are mostly suitable for neutral or weak acid conditions, for example, CN110257283A discloses a slow rooting tumor bacterium suitable for drug resistance, stress resistance and nitrogen fixation in Huang-Huai region and application thereof, but the slow rooting tumor bacterium has only single nitrogen fixation function, the acid resistance is not clear, and is not suitable for activating ferric phosphate, and CN118126903B discloses a multifunctional bacillus belicus capable of resisting acid and producing alkali, a microbial agent thereof and application thereof, and has the advantages of being alkaline-producing and acid-resisting, nitrogen fixation-free, and being a single strain, and being incapable of synchronously solving nitrogen and phosphorus co-deficiency. From the above, it is clear that the activity and stability suddenly decrease at a pH of less than 5.5, and the activation efficiency of iron phosphate is particularly low. Although nitrogen-fixing bacteria or phosphate-dissolving bacteria are reported, the method has not been known to realize stable and synergistic synthesis of high-efficiency nitrogen fixation and special activated ferric phosphate under a wide-range acidic gradient (pH 3.0-7.0), and is difficult to solve the problem of compounding of nitrogen and phosphorus deficiency in an acidic oligotrophic environment. Disclosure of Invention The invention aims to overcome the defects of the prior art and provides an acid-resistant nitrogen fixation-activated ferric phosphate synthetic flora, a compound microbial inoculum and application thereof. The synthetic flora shows remarkable synergistic effect in an acidic culture medium with pH of 3.0-7.0, can synchronously improve the effective nitrogen content and efficiently activate ferric phosphate to be effective phosphorus, has the functions of nitrogen fixation and phosphorus release which are gradually improved along with the enhancement of acidity in the range of pH of 3.0-6.0, still keeps stable function under the stress of strong acid (pH of 3.0), and is suitable for synchronous biological reinforcement of nitrogen and phosphorus nutrients in an acidic oligotrophic environment. In order to achieve the above purpose, the present invention provides the following technical solutions: The acid-resistant nitrogen fixation-activated ferric phosphate synthetic flora consists of microbial strains including Pseudomonas eastern lake (Pseudomonas donghuensis) N-13 and Pantoea agglomerans (Pantoea agglomerans) P-23, wherein the preservation number of the Pseudomonas eastern lake N-13 is CGMCC No.37624, and the preservation number of the Pantoea agglomerans P-23 is CGMCC No.37622. Preferably, the ratio of the effective viable count of the pantoea agglomerans P-23 to the effective viable count of the pseudomonas eastern lake N-13 is 1:1. A composite microbial inoculum comprising the synthetic flora. Preferably, the OD600 value of the synthetic flora in the composite microbial inoculum is more than or equal to 0.5. The scheme provides application of the synthetic flora or the composite microbial inoculum to biological nitrogen fixation and indissolvable phosphate activation and phosphorus release under an acidic condition, wherein the acidic condition comprises acidic soil or acidic water, the pH range of the acidic condition is 3.0-7.0, and the indissolvable phosphate is ferric phosphate or aluminum phosphate. The invention also provides a method for synchronously improving the content of effective nitrogen and effective phosphorus in the ac