CN-122012290-A - Composite microbial inoculum for mixed composting of sugarcane filter mud and sugarcane leaves and application method

Abstract

A composite microbial agent for mixing and composting filter mud and sugarcane leaves of sugarcane and an application method thereof belong to the technical field of microorganisms, the composite microbial agent comprises a high Wen Junji, a high Wen Junji, a medium temperature microbial agent, a two-stage inoculation method, wherein the high Wen Junji is prepared from thermophilic carbon monoxide streptomycete G-4-7, soil bacillus brevis G-5-2 and thermophilic chaetomium, the medium temperature microbial agent is prepared from trichoderma longibrachiatum, staphylococcus vortioides LH-403 and bacillus thuringiensis LMU-81. The composite microbial inoculum provided by the invention can effectively enhance the degradation of lignocellulose so as to promote the composting and the decomposition of the compost, further improve the humification degree of the compost through the biological enhancement effect, is beneficial to the nitrogen fixation effect of the compost and the improvement of the germination index of the compost, can promote the recycling of organic matters rich in lignocellulose, and provides a technical basis for the green treatment mode of agricultural wastes.

Inventors

• ZHOU HAO
• DING DE
• LI SHIQIANG
• CHEN SIMIN
• LI JIANBIN
• LI KAI
• ZHU BIYANG
• DENG LIGAO
• He Huiban
• LIANG HANZHU
• Xie Lianglong
• HUANG ZHI

Assignees

• 广西大学

Dates

Publication Date

20260512

Application Date

20260410

Claims (6)

1. 1. The composite microbial inoculum for mixed composting of sugarcane filter mud and sugarcane leaves is characterized by comprising a high Wen Junji and a medium Wen Junji, wherein the high Wen Junji and the medium Wen Junji are independently packaged, the high Wen Junji is suitable for a temperature range of composting more than or equal to 50 ℃, and the medium is suitable for a temperature range of composting less than 50 ℃; Gao Wen Junji is prepared from Streptomyces thermophilus (Streptomyces thermocarboxydus) G-4-7, brevibacillus soil (Brevibacillus agri) G-5-2 and Chaetomium thermophilum (Chaetomium thermophilum); the thermophilic streptomyces carbon monoxide (Streptomyces thermocarboxydus) G-4-7 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 36417 in the 10 th month of 2025; the Brevibacillus tumefaciens (Brevibacillus agri) G-5-2 is preserved in China general microbiological culture collection center (CGMCC) with a preservation number of 36418 in the 10 th month and 30 th year of 2025; The chaetomium thermophilum (Chaetomium thermophilum) is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC 3.17990; The mesophilic bacteria agent is prepared from staphylococcus vortioides (Staphylococcus warneri) LH-403, trichoderma longibrachiatum (Trichoderma longibrachiatum) and bacillus thuringiensis (Bacillus thuringiensis) LMU-81; The staphylococcus vortioides (Staphylococcus warneri) LH-403 is preserved in China general microbiological culture Collection center (CGMCC) with a preservation number of CGMCC No.36214 at the 10 th month 9 of 2025; The trichoderma longibrachiatum (Trichoderma longibrachiatum) is preserved in China center for type culture Collection of microorganisms, and the preservation number is CICC 41185; The Bacillus thuringiensis (Bacillus thuringiensis) LMU-81 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 36215 in the year 10 and the day 9 of 2025.
2. 2. The composite microbial inoculum for mixed composting of sugarcane filter mud and sugarcane leaves according to claim 1 is characterized in that the preparation method of the high-temperature microbial inoculum comprises the steps of inoculating thermophilic streptomyces carboxidans (Streptomyces thermocarboxydus) G-4-7, soil bacillus brevis (Brevibacillus agri) G-5-2 and thermophilic chaetomium (Chaetomium thermophilum) into a liquid culture medium respectively, culturing at 50 ℃, adjusting the colony quantity to 1X 10 8 CFU/mL, and mixing the strains in equal volume to obtain the high-temperature microbial inoculum.
3. 3. The composite microbial inoculum for mixed composting of sugarcane filter mud and sugarcane leaves according to claim 1 is characterized in that the preparation method of the mesophilic microbial inoculum comprises the steps of inoculating staphylococcus vortioides (Staphylococcus warneri) LH-403, trichoderma longibrachiatum (Trichoderma longibrachiatum) and bacillus thuringiensis (Bacillus thuringiensis) LMU-81 into a liquid culture medium respectively, culturing at 28 ℃, adjusting the colony quantity to 1X 10 8 CFU/mL, and mixing the strains in equal volumes to obtain the mesophilic microbial inoculum.
4. 4. A composting method using the composite microbial inoculum of claim 1, which is characterized in that two-stage inoculation is adopted, the high Wen Junji is inoculated in the initial stage of composting, and the medium-temperature microbial inoculum is inoculated after the composting enters a cooling period of less than 50 ℃.
5. 5. The method for composting a composite microbial inoculant according to claim 4, wherein the total inoculation amount of the composite microbial inoculant in the compost is 6% (v/w), and wherein the inoculation amounts of the high temperature microbial inoculant and the medium temperature microbial inoculant are 3% respectively.
6. 6. The use of the composite microbial agent of claim 1 in degrading lignocellulosic agricultural waste.

Description

Composite microbial inoculum for mixed composting of sugarcane filter mud and sugarcane leaves and application method Technical Field The invention belongs to the technical field of microbial agents, and particularly relates to a composite microbial agent for mixed composting of sugarcane filter mud and sugarcane leaves and an application method thereof. Background Economic development and population growth have driven the expansion of agricultural production scale, thereby producing a large amount of agricultural waste. The sugarcane is the sugar crop with the largest planting area in the world, is also the most important energy source and feed crop, and has higher ecological and economic values. Step-by-step mechanical harvesting is a mode of sugarcane mechanized harvesting operation, and refers to the steps of disassembling complete sugarcane harvesting procedures (such as sugarcane cutting, leaf stripping, sugarcane material transferring and the like) into a plurality of independent links, firstly completing sugarcane cutting, then conveying sugarcane materials to a transport vehicle by using a transfer machine, and finally completing sugarcane leaf and sugarcane tip separation by using special equipment. The stepwise mechanical harvesting is more suitable for the scenes of scattered land parcels, special crop variety characteristics or insufficient suitability of agricultural machinery in planting areas. The popularization of the step-by-step mechanical harvesting also faces a serious challenge, namely, the problem of timely processing by peeling sugarcane leaves. Because of the lack of mature and feasible high-value processing technology, most sugarcane leaves stripped by the step-by-step mechanical harvesting can only be stacked at an operation point, so that a large amount of land is occupied, and a high fire risk exists. With further popularization of the stepwise mechanical harvesting, the sugarcane leaf production amount is continuously increased. Therefore, the search and the realization of the on-site resource utilization of sugarcane leaves have become the key for comprehensively promoting the sugarcane harvesting of sugar materials. The sugarcane filter mud is a byproduct generated in the sugarcane sugar production process, and is an organic mixture which is precipitated and separated out after the sugarcane mixed juice is subjected to the action of a filtering clarifier. The filter mud is rich in organic matters, fertilizer nutrients such as nitrogen, phosphorus, potassium and the like, and medium trace elements such as calcium, magnesium, sulfur and the like, and is a high-quality organic fertilizer raw material. However, current sludge utilization is mainly based on simple stack retting or direct returning to the field. Due to the lack of the high-efficiency decomposition technology, the utilization efficiency is quite low, and the problems of plant diseases and insect pests, soil-borne diseases, root burning, seedling burning and the like are easily caused. In recent years, exploration practices for preparing organic fertilizer from filter mud have been developed in the industry, but the problems of long fermentation period, insufficient microbial activity, unstable fertilizer efficiency and the like in the prior art still generally exist, the additional value of the product is limited, and the efficient utilization of the sugarcane filter mud is difficult to support. The sugarcane leaf and the sugarcane filter mud are byproducts of sugarcane sugar production, are rich in lignocellulose, can be used as organic fertilizer raw materials, are complementary in carbon-nitrogen ratio after being mixed, have balanced nutrition elements, and can realize comprehensive utilization of wastes by adding fermentation microorganisms to produce organic fertilizer. However, in the fermentation degradation process, the difficult degradation of lignocellulose, particularly lignin components, and the aerobic composting of solid wastes rich in lignocellulose often show lower degradation efficiency. In order to promote the degradation of lignocellulose and shorten the process, high-temperature composting is commonly adopted for fermentation at present. The high temperature period of the compost is a key stage of biological decomposition of organic matters and harmless treatment of the compost, but microorganisms are often influenced by high temperature factors at the stage, population quantity and activity are generally reduced, and the residence time of the high temperature period is short, so that the rapid decomposition of the organic matters is limited. Although a plurality of composite bacterial agents for promoting composting and a technology for composting mixed with sugarcane filter mud and sugarcane leaves are also described in the prior art, the degradation capability of the existing composite bacterial agents for the lignocellulose of the mixed composting of the sugarcane filter mud and the sugarcan