CN-122012291-A - Composite microbial agent for promoting corn growth based on rhizosphere micro-food net and application

Abstract

The invention discloses a composite microbial agent for promoting corn growth based on rhizosphere micro-food nets and application thereof, and belongs to the technical field of agricultural microbial agents. The invention provides a composite microbial agent for promoting corn growth and improving corn yield based on rhizosphere micro-food nets, which consists of Bacillus wiedmannii JN.2, flavobacterium ginsenosidimutans JN.3 and Rossellomorea aquimaris L-3 strains, is matched with a specific cultivation mode, namely straw returning covering and seeding strip cultivation, can remarkably increase the quantity of rhizosphere microbial populations, directionally regulate and control the rhizosphere micro-food nets, improve the soil structure, accelerate nutrient release, promote the effective conversion of nutrients in the micro-food nets and the transmission of the nutrients to corn plants, promote corn growth and improving corn yield, and is environment-friendly, sustainable, convenient to use, simple to operate and high in economic benefit, and meets the requirements on green and high-quality agricultural products.

Inventors

• LIU TING
• JIANG NAN
• JIAO JIAGUO
• LI GEN
• LI HUIXIN

Assignees

• 南京农业大学三亚研究院

Dates

Publication Date

20260512

Application Date

20260410

Claims (8)

1. 1. The composite microbial agent for promoting the growth of corn based on the rhizosphere micro-food net is characterized by comprising Bacillus wiedmannii JN.2, flavobacterium ginsenosidimutans JN.3 and Rossellomorea aquimaris L-3 strains; Bacillus wiedmannii JN.2, classified and named Bacillus wiedmannii JN.2, is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20252922 at 12 months and 17 days of 2025; flavobacterium ginsenosidimutans JN.3, classified and named Flavobacterium ginsenosidimutans JN.3, is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20252923 at 12 months and 17 days of 2025; Rossellomorea aquimaris L-3, classified as Rossellomorea aquimaris L-3, was deposited in China center for type culture Collection (CCTCC NO: M20241623) at 22 th month 07 of 2024.
2. 2. The composite microbial agent for promoting corn growth based on the rhizosphere micro-food network of claim 1, wherein the microbial activity ratio of Bacillus wiedmannii JN.2: flavobacterium ginsenosidimutans JN.3: rossellomorea aquimaris L-3 in the composite microbial agent is 1:1:1.
3. 3. The composite microbial agent for promoting corn growth based on the rhizosphere micro-food web according to claim 1, wherein the viable count of three strains in the composite microbial agent is 1X 10 9 CFU/mL.
4. 4. The method of claim 1 to 3, wherein the composite microbial agent is used for promoting the growth of corn and increasing the yield of corn.
5. 5. The application of the composite microbial agent for promoting corn growth based on the rhizosphere micro-food network according to claim 4, wherein the application method is root irrigation after sowing.
6. 6. The application of the composite microbial agent for promoting corn growth based on the rhizosphere micro-food network according to claim 5, wherein the microbial agent is diluted by 80-150 times before root irrigation.
7. 7. The application of the composite microbial agent for promoting corn growth based on the rhizosphere micro-food network according to claim 5, which is characterized in that seeds are sown on straw return covered and sown on land areas with tilled strips.
8. 8. The application of the composite microbial agent for promoting corn growth based on the rhizosphere micro-food network according to claim 6, wherein the root irrigation amount is 30L of composite microbial agent diluent/mu.

Description

Composite microbial agent for promoting corn growth based on rhizosphere micro-food net and application Technical Field The invention relates to the technical field of agricultural microbial agents, in particular to a composite microbial agent for promoting corn growth based on rhizosphere micro-food nets and application thereof. Background Rhizosphere is a micro-domain soil environment which is strongly influenced by plant root system activity, and is a core interface of soil-plant system substance exchange. The rhizosphere micro-food net is driven by root exudates and comprises a complex ecological network of multi-nutrient organisms such as bacteria, fungi, protozoa, nematodes and the like. The network dominates the mineralization and turnover of rhizosphere nutrients through interaction such as predation, decomposition and the like, and has a vital regulation and control effect on crop nutrient acquisition, stress resistance and growth and development. The corn is used as a global important grain and feed crop, and has important significance for promoting high and stable yield. However, under the modern intensive planting mode, the imbalance of the corn rhizosphere microecological system is caused by a single cultivation system and excessive fertilizer input for a long time, and the method is characterized in that the soil biodiversity is reduced, the number of key indication groups such as nematodes is reduced, the microbial community function is degraded, the key ecological processes such as soil organic matter decomposition and nutrient circulation are blocked, and the soil biological fertility is obviously reduced. This has become a potential bottleneck limiting further improvement of corn yield and green transformation in agriculture. Currently, aiming at microbial technology for improving crop growth, the development of single or composite microbial agents with direct growth-promoting functions (such as secretion of growth hormone, nitrogen fixation and phosphorus dissolution) is focused on. Although the traditional microbial inoculum can improve crop growth to a certain extent, the action mode of the traditional microbial inoculum is often limited to a direct path of a microbial inoculum-plant, and the problems of unstable effect, short duration and the like are generally existed. The root cause is that the methods neglect the cooperative interaction among organisms at all levels in the rhizosphere micro-food network, fail to integrate the functions of the microbial inoculum into a wider soil ecological network, and fail to start and maintain an 'ecological engine' driving the efficient circulation of nutrients. However, how to construct an effective functional microbial agent combination, and systematically rebuild and optimize a corn rhizosphere micro-food net through matched agricultural measures so as to realize stable and continuous promotion of corn growth, and a mature and reliable technical scheme still lacks at present. Therefore, a composite microbial agent capable of accurately regulating and controlling rhizosphere micro-food nets is developed, so that corn growth is promoted, yield is improved, soil nutrient dynamics is optimized, and the composite microbial agent has important theoretical significance and wide application prospect. Disclosure of Invention The invention aims to provide a composite microbial agent for promoting corn growth based on a rhizosphere micro-food network and application thereof, so as to solve the problems of single function, unstable action effect and short duration of the traditional microbial agent, and provide an effective scheme for realizing green yield increase of crops by directionally regulating and controlling the rhizosphere micro-food network. To achieve the above object, the present invention provides a composite microbial agent for promoting corn growth based on rhizosphere micro-food nets, which is composed of Bacillus wiedmanniijn.2, flavobacterium ginsenosidimutans jn.3 and Rossellomorea aquimarisL-3 strains. Preferably, the bacterial activity ratio of Bacillus wiedmanniiJN.2 to Flavobacterium ginsenosidimutansJN.3: rossellomorea aquimarisL-3 in the composite microbial agent is 1:1:1. Preferably, the viable count of the three strains in the compound microbial agent is 1X 10 9 CFU/mL. The application of the composite microbial agent for promoting the growth of corn based on the rhizosphere micro-food network is the application in promoting the growth of corn and improving the yield of corn. Preferably, the application method is root irrigation after sowing. Preferably, the bacterial liquid is diluted before root irrigation, and the dilution multiple is 80-150 times. Preferably, the seeds are sowed on the land where the straw returns to the row to cover and sow the strip to plough. Preferably, the root irrigation amount is 30L of the composite microbial agent diluent per mu. Bacillus wiedmannii JN.2, classified and named Bacillus wiedmannii JN