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CN-122012292-A - Microorganism composite microbial agent and application thereof in coverage planting mode

CN122012292ACN 122012292 ACN122012292 ACN 122012292ACN-122012292-A

Abstract

The invention relates to a microbial composite microbial agent and application thereof in a coverage planting mode, and belongs to the technical field of microorganisms. The microbial synthetic flora comprises bacillus subtilis JZ23081 and bacillus bailii JZ23083, the effective viable count ratio of the two strains is 1:1, and the effective viable count of the composite microbial agent prepared by the synthetic flora is more than or equal to 5.0X10 8 /mL. The invention also provides application of the synthetic flora and the composite microbial inoculum in corn coverage planting, and provides products for promoting corn growth and improving soil nutrient conversion rate. The composite microbial inoculum can obviously promote the growth of corns in seedling stage, promote the soil nutrient conversion rate and the microbial activity, effectively solve the problems of late corns emergence, low emergence rate, poor seedling growth and the like caused by slow straw decomposition and low ground temperature in northern early spring coverage planting, realize the synergistic effect of corn strong seedlings and soil improvement, is particularly suitable for the production of spring corns in a thin-layer black soil area, and has important practical significance for agricultural quality improvement and ecological protection.

Inventors

  • BAO XUELIAN
  • JIANG ZHIYANG
  • WANG YU
  • CAO XIA
  • DENG FANGBO
  • XU XIN
  • ZHOU WENRUI
  • ZHOU JINXIN

Assignees

  • 中国科学院沈阳应用生态研究所

Dates

Publication Date
20260512
Application Date
20260410

Claims (10)

  1. 1. A microbial synthetic flora comprising the following microbial strains: Bacillus subtilis (Bacillus subtilis) JZ23081 is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) with the address of North Star Xiyu No. 1, 3 in the Korean area of Beijing, and with the preservation number of CGMCC No.36304; bacillus belicus (Bacillus velezensis) JZ23083 was deposited with China general microbiological culture Collection center (CGMCC) at the 10 th month 23 th year 2025, and was deposited with the Seagaku-1, no.3, the Ministry of Kogyo North Star of Beijing, and with a collection number of CGMCC No.36306.
  2. 2. The microorganism-producing strain of claim 1, wherein the ratio of the number of viable bacteria of Bacillus subtilis JZ23081 to Bacillus bailii JZ23083 in the microorganism-producing strain is 1:1.
  3. 3. A microbial composite agent comprising a synthetic flora according to any one of claims 1-2.
  4. 4. The microbial composite agent according to claim 3, wherein the effective viable count of the composite agent is not less than 5.0X10 8 /mL.
  5. 5. Use of a microbial synthetic flora according to any one of claims 1-2 or a microbial composite inoculant according to any one of claims 3-4 in crop planting.
  6. 6. The use of a microbial synthetic flora or a microbial composite agent according to claim 5, wherein the crop is corn and the crop planting pattern is a blanket planting pattern.
  7. 7. The use of a microbial synthetic flora or a microbial composite inoculant according to claim 5, wherein the microbial synthetic flora or microbial composite inoculant is used for promoting corn growth.
  8. 8. The use of a microbial synthetic flora or a microbial composite inoculant according to claim 5, wherein the microbial synthetic flora or microbial composite inoculant is used for improving the soil nutrient conversion rate.
  9. 9. A product for promoting corn growth comprising the microbial synthetic flora according to any one of claims 1-2 or the microbial complex inoculant according to any one of claims 3-4.
  10. 10. A product for increasing the nutrient conversion of soil comprising the microbial synthetic flora according to any one of claims 1-2 or the microbial composite inoculant according to any one of claims 3-4.

Description

Microorganism composite microbial agent and application thereof in coverage planting mode Technical Field The invention relates to the technical field of microorganisms, in particular to a microbial composite microbial agent and application thereof in a coverage planting mode. Background The corn stalk covering and returning technology is a common soil fertility improvement means in the current agricultural production. The technology can realize the stable cultivation layer soil structure, increase the soil nutrient, reduce the agricultural production cost and other multiple benefits by covering the corns after harvesting on the ground surface for returning. Different from straw coverage, crop coverage is an advanced stage of protective cultivation, and leguminous coverage crops are introduced into a protective cultivation system, and the core advantages of the crop coverage are that soil nitrogen supply is improved through biological nitrogen fixation, and organic matters are increased to improve soil nutrient circulation. However, in early spring sowing in north, the straws are slowly decomposed in soil due to the limitation of climate conditions, the expected soil fertility and nutrient supply effect are difficult to realize in a short period, and meanwhile, the limitations of unsynchronized residual nutrient release and crop demands exist. Under the conditions of low temperature and insufficient supply of soil nutrients, seed germination is delayed, so that the seed emergence rate and seedling root system development are affected, and the absorption of water and nutrients by plants is further affected. In order to alleviate the adverse effects of low temperature on germination and emergence of spring corn seeds, the main measures at present comprise film-covered planting, seed initiation treatment, seeding period adjustment and the like. The seed initiation treatment mostly adopts a penetration regulator, a plant growth regulator, a chemical bactericide or a single nutrition preparation, although the seed emergence rate can be improved to a certain extent, the function is single, the seed initiation treatment only acts on the surfaces of seeds or seedlings, the microecological environment of rhizosphere soil cannot be improved, and particularly in a covering planting mode, the synergistic effect of strong seedlings and soil improvement is difficult to realize. Disclosure of Invention The invention aims to provide a microbial synthetic flora and a microbial composite microbial agent, and further provides an application of the microbial synthetic flora or the microbial composite agent in a coverage planting mode so as to solve the problems in the prior art. On the basis of straw covering and crop covering protective cultivation, the inventor develops a microbial synthetic flora, and two functional microbial viable bacteria, namely bacillus subtilis (Bacillus subtilis) JZ23081 and bacillus bailii (Bacillus velezensis) JZ23083, are screened and compounded. Specifically, the invention is realized through the following scheme: in a first aspect, the invention provides a synthetic flora comprising the following microbial strains: Bacillus subtilis (Bacillus subtilis) JZ23081 is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) with the address of North Star Xiyu No. 1, 3 in the Korean area of Beijing, and with the preservation number of CGMCC No.36304; bacillus belicus (Bacillus velezensis) JZ23083 was deposited with China general microbiological culture Collection center (CGMCC) at the 10 th month 23 th year 2025, and was deposited with the Seagaku-1, no.3, the Ministry of Kogyo North Star of Beijing, and with a collection number of CGMCC No.36306. Wherein the effective viable count ratio of bacillus subtilis JZ23081 and bacillus bailii JZ23083 in the synthetic flora is 1:1. In a second aspect, the invention provides a microbial composite inoculant comprising a first proposed synthetic population. Wherein the effective viable count of the composite microbial inoculum is more than or equal to 5.0X10 8/mL. In a third aspect, the present invention provides the use of the synthetic flora set forth in the first aspect or the complex microbial inoculum set forth in the second aspect as set forth above in crop planting. Preferably, the crop planting mode is a blanket planting mode. Preferably, the crop is corn. Preferably, the synthetic flora or complex inoculant is used in promoting the growth of maize plants. Preferably, the synthetic flora or the composite microbial inoculum is applied to improving the soil nutrient conversion rate. In a fourth aspect, the invention provides a product for promoting corn growth, comprising the synthetic flora as set forth in the first aspect or the composite microbial inoculant as set forth in the second aspect. In a fifth aspect, the present invention provides a product for improving the nutrient convers