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CN-122012295-A - Composite microbial inoculum separated from sour soup and having adsorption function as well as preparation method and application thereof

CN122012295ACN 122012295 ACN122012295 ACN 122012295ACN-122012295-A

Abstract

The invention discloses a composite microbial agent with an adsorption function separated from sour soup, and a preparation method and application thereof, and belongs to the technical field of microorganisms, in particular relates to a composite microbial agent with an adsorption function separated from sour soup, and the composite microbial agent comprises lactobacillus plantarum MJ-S1 and lactobacillus casei MJ-P1, wherein the preservation number of the lactobacillus plantarum MJ-S1 is CCTCC NO: M2026044, and the preservation number of the lactobacillus casei MJ-P1 is CCTCC NO: M2026043. The application of the composite microbial agent in adsorbing microplastic. The composite microbial inoculum composed of the lactobacillus plantarum MJ-S1 and the cheese bacillus MJ-P1 has a micro-plastic adsorption function, and can be used for preparing red sour soup with good flavor by fermentation.

Inventors

  • LI WEI
  • XIA YIXUAN
  • XIAO LUYAO
  • RUI XIN
  • Bao Mengxiao
  • WU YI

Assignees

  • 南京农业大学三亚研究院
  • 南京农业大学

Dates

Publication Date
20260512
Application Date
20260416

Claims (9)

  1. 1. A composite microbial agent with adsorption function separated from sour soup, which is characterized by comprising lactobacillus plantarum (Lactiplantibacillus plantarum) MJ-S1 and lactobacillus casei (Lacticaseibacillus casei) MJ-P1; The Lactobacillus plantarum MJ-S1 is preserved in China center for type culture Collection (China center for type culture Collection) in 2026, 01 month and 08, and has a preservation address of China university of Wuhan, and a preservation number of CCTCC NO: M2026044; The cheese bacillus MJ-P1 is preserved in China center for type culture Collection (China, university of Wuhan, china, and CCTCC NO: M2026043) at 2026 and 01 month 08.
  2. 2. The composite microbial inoculant according to claim 1, wherein the mass ratio of lactobacillus plantarum MJ-S1 to lactobacillus casei MJ-P1 in the composite microbial inoculant is 2:1.
  3. 3. The composite microbial agent according to claim 1, wherein the total effective viable count of the composite microbial agent is not less than 1 x 10 10 CFU/mL.
  4. 4. The preparation method of the composite microbial inoculum according to any one of claims 1-3 is characterized by comprising the following steps of mixing lactobacillus plantarum MJ-S1 bacterial liquid and lactobacillus casei MJ-P1 bacterial liquid according to a mass ratio of 2:1 to prepare the composite microbial inoculum.
  5. 5. The method according to claim 4, wherein the effective viable count of the Lactobacillus casei MJ-S1 strain solution is 1X 10 9 CFU/mL, and the effective viable count of the Lactobacillus casei MJ-P1 strain solution is 1X 10 9 CFU/mL.
  6. 6. The use of a composite microbial agent according to any one of claims 1 to 3 for adsorbing microplastic, wherein the microplastic is PS.
  7. 7. The application of the composite microbial inoculum according to any one of claims 1-3 in preparation of a preparation with a micro-plastic adsorption function, wherein the micro-plastic is PS.
  8. 8. The use of a complex microbial inoculant according to any one of claims 1-3 for the preparation of red soups.
  9. 9. The application of claim 8, wherein the application is specifically that capsicum and tomato are taken, broken, salt is added to obtain fermentation raw materials, the composite microbial inoculum is inoculated into the fermentation raw materials according to an inoculum size of 6% (v/v), and the fermentation raw materials are stood for 7d at 32 ℃ to obtain the red acid soup.

Description

Composite microbial inoculum separated from sour soup and having adsorption function as well as preparation method and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a composite microbial agent with an adsorption function, which is separated from sour soup, and a preparation method and application thereof. Background Microplastic refers to plastic particles with the diameter smaller than 5mm, the microplastic can exist in soil, fresh water, sediment, atmosphere, drinking water and human body, and along with the surge of global plastic consumption and the defect of waste management, the pollution of the microplastic is rapidly promoted and is widely distributed in all environments. Prolonged exposure to microplastic is a potential risk to human health, as the presence of microplastic in plastic products may result in contact, food and air, through ingestion, inhalation and skin contact. In all biological systems, exposure of microplastic may lead to particle toxicity, with concomitant oxidative stress, inflammatory lesions, and increased uptake or translocation, affecting human health. In the field of recent years, the use of probiotics with micro-plastic adsorption function is mentioned in patents. Although probiotics have a certain effect in the field of adsorption of microplastic, the effect of the probiotic composition is limited, for example, the publication number is CN 117089486A, the name is Lactobacillus plantarum DT55 and the application thereof in preparing products with the function of adsorption and removal of microplastic, the lactobacillus plantarum (Lactiplantibacillus plantarum) DT55 separated from healthy human excrement can adsorb microplastic, but the effect of probiotic combination and compounding the function of adsorption of microplastic is not mentioned, the publication number is CN119372103A, namely the microplastic adsorbent prepared by compounding lactobacillus plantarum MPs-68 with adsorption microplastic and uric acid degradation and the application thereof, the microorganism adsorbent prepared by compounding lactobacillus plantarum MPs-68 separated from marine products and lactobacillus curvatus (Latilactobacillus curvatus) MPA01 is mentioned, but specific probiotic combination compounding examples are not mentioned, and the used compound strain is lactobacillus plantarum and lactobacillus curvatus. The microplastic is used as a widely existing pollutant in the environment, and micron-sized particles of the microplastic can enter human bodies through food chains, so that potential threats are formed to intestinal microecological balance and host health. Research shows that specific probiotics can effectively capture and degrade micro plastic particles through mechanisms such as biomembrane wrapping, surface adsorption, extracellular polymer combination and the like, so that retention and absorption of the micro plastic particles in intestinal tracts are reduced. In addition, probiotics can indirectly reduce oxidative stress and inflammatory response caused by microplastic through regulating intestinal barrier function and maintaining flora steady state. In the field of food processing or environmental treatment, probiotics are added as biological adsorbents, so that the probiotics can be combined before being absorbed by intestinal tracts and discharged out of the body through feces, and the toxic damage to organisms is obviously inhibited. Therefore, the method for screening the probiotics strain with the capability of efficiently adsorbing the microplastic has important application prospect in the fields of environmental remediation and health protection. Disclosure of Invention In order to solve the technical problems, the invention provides a composite microbial agent with an adsorption function, which is separated from sour soup, a preparation method and application thereof, and the provided composite microbial agent consisting of lactobacillus plantarum (Lactiplantibacillus plantarum) MJ-S1 and cheese bacillus (Lacticaseibacillus casei) MJ-P1 has a micro-plastic adsorption function and can be used for preparing red sour soup with good flavor by fermentation. The invention provides a composite microbial inoculum with an adsorption function, which is separated from soups, and comprises lactobacillus plantarum MJ-S1 and lactobacillus casei MJ-P1, wherein the lactobacillus plantarum MJ-S1 is preserved in China center for type culture collection at the year 2026 and the month 08, the preservation address is China, wuhan university, the preservation number is CCTCC NO: M2026044, the lactobacillus casei MJ-P1 is preserved in China center for type culture collection at the year 2026 and the month 08, the preservation address is China, wuhan university, and the preservation number is CCTCC NO: M2026043. Preferably, the mass ratio of the lactobacillus plantarum MJ-S1 to the lactobacillus casei MJ-P1 in