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CN-122012298-A - Deep sea cold-resistant thiomonas and application thereof

CN122012298ACN 122012298 ACN122012298 ACN 122012298ACN-122012298-A

Abstract

The invention relates to the technical field of microorganisms, in particular to deep-sea thiomonas and application thereof. The strain is Sulfurimonas iocasae FCS preserved in China general microbiological culture collection center (CGMCC) No.46387. The strain has the functions of carbon fixation, denitrification and dephosphorization and the application of deep sea thiomonas strain with multi-vitamin production in the fields of environmental restoration, biological product production and the like.

Inventors

  • SUN QINGLEI
  • YAN XIAOMAN
  • SUN LI

Assignees

  • 中国科学院海洋研究所

Dates

Publication Date
20260512
Application Date
20250408

Claims (7)

  1. 1. A deep sea cold-resistant thiomonad is characterized in that the strain is Sulfurimonas iocasae FCS which is preserved in China general microbiological culture collection center with the preservation number of CGMCCNo.46387.
  2. 2. The use of deep sea sulfur cold-tolerant monad as claimed in claim 1, characterized in that the strain is used in environmental carbon fixation by using carbon dioxide.
  3. 3. A process for preparing deep-sea cold-resistant thiomonas strain according to claim 1, wherein said strain is used for denitrification and/or dephosphorization in the environment.
  4. 4. The application of deep sea cold-resistant sulfur monad as claimed in claim 1, wherein the strain is applied to degrading nitrogen and phosphorus pollutants in environment.
  5. 5. The application of deep sea cold-resistant sulfur monad as claimed in claim 4, wherein the strain is used for degrading nitrogen and phosphorus pollutants in low-temperature sewage or soil.
  6. 6. The use of deep-sea sulfur-tolerant bacteria as claimed in claim 1, characterized in that the strain is used for producing biological products.
  7. 7. The application of deep sea cold-resistant thiomonas according to claim 6, wherein the strain is applied to the preparation of protein and vitamins.

Description

Deep sea cold-resistant thiomonas and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to deep-sea thiomonas and application thereof. Background Under the current environmental problems and biotechnology development demands, it is of great importance to find microorganisms which are efficient and can adapt to special environmental conditions. The microbial activity in the low-temperature environment plays a key role in the aspects of low-temperature industrial wastewater treatment and the like. Meanwhile, the carbon fixation, denitrification and dephosphorization functions are important for relieving the greenhouse effect, purifying the water eutrophication and other environmental problems, and the microorganism can produce vitamins, so that the method has potential application value in the fields of biological products and medicines. However, a bacterial strain which has many of the above-mentioned excellent properties and which effectively functions at low temperatures has not been found yet. Disclosure of Invention The invention aims to provide a novel bacterial strain which can grow in a low-temperature environment and has the functions of carbon fixation, denitrification and dephosphorization and multi-vitamin production so as to meet the various demands of environmental restoration, biological product production and the like. In order to achieve the above purpose, the invention adopts the technical proposal that The deep sea cold-resistant thiomonas strain is Sulfurimonas iocasae FCS which is preserved in China general microbiological culture collection center (CGMCC) No.46387. The application of the deep sea cold-resistant thiomonas is that the strain is applied to environmental carbon fixation by utilizing carbon dioxide. Use of said deep sea sulfur cold tolerant monad, said strain for denitrification and/or dephosphorization in the environment. The application of the deep sea cold-resistant thiomonas strain is that the strain is applied to removing nitrogen and phosphorus pollutants in the environment. The strain is applied to degradation of nitrogen and phosphorus pollutants in low-temperature sewage or soil. The application of the deep sea cold-resistant thiomonas strain in the production of biological products. The strain is applied to the preparation of proteins and vitamins. The invention has the following advantages: The bacterial strain can grow and perform multiple functions in a low-temperature environment, widens the application range (5-20 ℃) of microorganisms in the low-temperature environment, and provides a new way for environment restoration and biotechnology development in low-temperature areas. The carbon fixation, denitrification and dephosphorization functions are beneficial to solving the current environmental problems, reducing the emission of greenhouse gases, purifying water and having remarkable environmental benefits. The function of producing protein and multiple vitamins provides a new microorganism source for the production of biological products, reduces the production cost of the protein and the vitamins, and has potential economic benefit. Description of the drawings FIG. 1 shows a temperature growth test of strain FCS5 according to an embodiment of the present invention FIG. 2 is a carbon dioxide fixation experiment of strain FCS5 according to an embodiment of the present invention FIG. 3 shows denitrification and dephosphorization experiments of the strain FCS5 according to an embodiment of the present invention Detailed Description The invention is further illustrated below with reference to examples. The examples are intended to illustrate the invention and not to limit it in any way. Example 1 Acquisition of thiomonas FCS 5: 1. Isolation of strains And (3) enriching and culturing the potential armoured shrimp seta sample collected by a scientific research ship in cold spring in south China sea for 30 days by using an MJH culture medium at 10 ℃, and separating and culturing by using a limiting dilution method after the culture medium is turbid. After the strain was purified, it was placed in 15% glycerol and stored for a long period at-80 ℃. The MJH culture medium comprises the following ：NaCl 30.0g/L,K2HPO4 0.14g/L,CaCl2·2H2O 0.14g/L,MgSO4·7H2O 3.4g/L,MgCl2·6H2O 4.18g/L,KCl 0.33g/L,NH4Cl 0.25g/L,Fe(NH4)2(SO4)2·6H2O 0.01g/L,NaNO3 2g/L,NaHCO3 1.5g/L; trace element solutions (trace mineral element solutions: aminotriacetic acid 1.5g/L,MgSO4·7H2O 3.0g/L,MnSO4·H2O 0.5g/L,NaCl 1.0g/L,FeSO4·7H2O 0.1g/L,CoSO4·7H2O0.1g/L,CaCl2·2H2O 0.1g/L,ZnSO4·7H2O 0.1g/L,CuSO4·5H2O 0.01g/L,AlK(SO4)2 0.01g/L,H3BO3 0.01g/L,Na2MoO4·2H2O 0.01g/L. is used for dissolving aminotriacetic acid, naOH solution is used for adjusting the pH to 6.5, various components are sequentially added, and finally the pH is adjusted to 7.0.), 1mL of vitamin solution (vitamin solution: biotin 2.0mg/L, folic acid 2.0mg/L, pyridoxine hydrochloride 10