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CN-122012299-A - Bacterial strain with high nitrite tolerance and ammonium production function, bacterial agent, preparation method and application of bacterial agent

CN122012299ACN 122012299 ACN122012299 ACN 122012299ACN-122012299-A

Abstract

A bacterial strain and a bacterial agent with high nitrite tolerance and an ammonium generating function and a preparation method and application of the bacterial agent relate to a bacterial strain and a bacterial agent and a preparation method and application of the bacterial agent. The invention aims to solve the problems of low nitrogen availability, excessive use of chemical nitrogenous fertilizer, ecological degradation of soil and poor tolerance of the traditional denitrification process. The strain is named as streptomyces circulans Streptomyces anulatus and is preserved in China center for type culture collection, wherein the address is the university of Wuhan, china, the preservation date is 2025, 5 and 12 days, and the preservation number is CCTCC NO: M20251026. The invention belongs to the technical field of microorganisms.

Inventors

  • WANG XIN
  • SONG FUQIANG
  • XU CHONGXIN
  • LIU SIRUI
  • WANG XIN
  • ZHANG CONGMIN
  • HUANG QIANCHI

Assignees

  • 黑龙江大学

Dates

Publication Date
20260512
Application Date
20251013

Claims (8)

  1. 1. A strain with high nitrite tolerance and ammonium production function is characterized in that the strain is named as streptomyces circulans Streptomyces anulatus and is preserved in China center for type culture collection, and the preservation number is CCTCC NO: M20251026.
  2. 2. The strain having high nitrite tolerance and an ammonium-producing function according to claim 1, wherein the ammonium-producing function is represented by continuously and stably producing ammonium nitrogen under the condition that nitrate or nitrite is used as a nitrogen source, and the average ammonium-producing efficiency under the condition of nitrate is 3.1 to 4.3 mg/(l.h), and the average ammonium-producing efficiency under the condition of nitrite is 0.92 to 1.02 mg/(l.h).
  3. 3. A microbial inoculum prepared by using the strain of claim 1, wherein the fermentation broth of Streptomyces roseus Streptomyces anulatus255 is concentrated and then mixed with trehalose, glycerol, D-ascorbic acid, potassium sorbate and sodium dehydroacetate, wherein after each liter of fermentation broth is concentrated to 150 mL, trehalose 4g, glycerol 2 g, D-ascorbic acid 0.5 g, potassium sorbate 0.3 g and sodium dehydroacetate 0.04 g are added and the volume is fixed to 200 mL.
  4. 4. A preparation method of a microbial agent with high nitrite tolerance and ammonium generation function is characterized by comprising the following specific steps: Step 1, inoculating streptomyces circulans Streptomyces anulatus255 into an LB bacterial culture medium for culturing; and step 2, re-suspending and adding a protective agent to prepare the microbial inoculum.
  5. 5. The method for producing a microbial inoculum having high nitrite tolerance and producing ammonium as claimed in claim 3, wherein the temperature in the LB liquid medium in the step 1 is 30 ℃, the culturing time is 48 hours, and the viable bacteria concentration in the microbial inoculum is 2.5X10 8 cfu/mL.
  6. 6. The method of claim 3, wherein the protective agent in step 2 is trehalose, glycerol, D-ascorbic acid, potassium sorbate or sodium dehydroacetate.
  7. 7. The application of the microbial inoculum with high nitrite tolerance and ammonium generation function is characterized in that the microbial inoculum is used for nitrite-containing wastewater treatment, nitrogen recovery and ecological restoration.
  8. 8. The application of the microbial inoculum with high nitrite tolerance and ammonium production function is characterized in that the ammonium nitrogen content of the soil is increased by 2.19-3.05 times in 15 days after the microbial inoculum is applied to the soil, the ammonium nitrogen content of the soil is increased by 1.44-1.64 times in 30 days, the dry weight of plants is increased by 12-35%, the plant height is increased by 10-30%, and the nitrogen content of leaves is increased by 38-43%.

Description

Bacterial strain with high nitrite tolerance and ammonium production function, bacterial agent, preparation method and application of bacterial agent Technical Field The invention relates to a strain, a microbial agent, a preparation method and application of the microbial agent, and belongs to the technical field of microorganisms. Background Ammonium nitrogen is one of the main sources of nitrogen in the soil, and is a direct and rare source of nitrogen for microorganisms in the environment, especially for those microorganisms in the environment that cannot directly use nitrate as a source of nitrogen. Ammonium nitrogen is also an important nitrogen source for plants, when plants use ammonium nitrogen as a nitrogen source, hydrogen ions are released to improve the alkaline environment of soil, and the utilization efficiency of ammonium by plants is higher than that of nitrate, which is also a great advantage of ammonium nitrogen. Along with the continuous expansion of industrial and agricultural wastewater, livestock and poultry breeding tail water and municipal sewage treatment systems, nitrite has become one of the main causes of water eutrophication and public health risks. Nitrite inhibits hemoglobin oxygen carrying function and has an acute lethal concentration of onlyThe high-concentration nitrite not only damages the microecological balance of the rhizosphere, but also causes soil nitrogen loss and nitrogen loss through denitrificationAnd (5) discharging. Therefore, the method can be used for developing ammonium which can stably survive under high nitrite stress and efficiently convert nitrogen into ammonium which can be absorbed and utilized by crops) Is particularly important. On the application level, the biological microbial inoculum has the advantages that ① can quickly convert acute toxic nitrite into ammonium for phytoplankton in aquaculture water, reduce water changing frequency and tail water discharge, and ② can maintain rhizosphere nitrogen circulation steady state under high nitrite stress and reduce crop nitrogen utilization rate by inoculating the functional microbial inoculum through a biological strengthening technology in a soil restoration sceneCompared with the conventional nitrification-denitrification process, ③ has the advantages of no need of strict staged control of the microbial inoculum process, low running cost and low technical requirements, and is suitable for decentralized rural sewage treatment and in-situ purification of facility fishery tail water. Disclosure of Invention The invention aims to solve the problems of low nitrogen availability, excessive use of chemical nitrogen fertilizer, ecological degradation of soil and poor tolerance of the traditional denitrification process, and further provides a bacterial strain and a bacterial agent with high nitrite tolerance and ammonium generation function, and a preparation method and application of the bacterial agent. The invention adopts the technical scheme that the strain with high nitrite tolerance and ammonium production function is named as streptomyces circulans Streptomyces anulatus and is preserved in China center for type culture collection, the address is China university of Wuhan, the preservation time is 2025 years, 5 months and 12 days, and the preservation number is CCTCC NO: M20251026. The microbial inoculum with high nitrite tolerance and ammonium production function is prepared by concentrating fermentation liquor of streptomyces circulans Streptomyces anulatus, mixing the fermentation liquor with trehalose, glycerol, D-ascorbic acid, potassium sorbate and sodium dehydroacetate, concentrating each liter of fermentation liquor to 150 mL, adding trehalose 4 g, glycerol 2g, D-ascorbic acid 0.5 g, potassium sorbate 0.3 g and sodium dehydroacetate 0.04 g, and fixing the volume to 200 mL. The invention relates to a preparation method of a microbial inoculum with high nitrite tolerance and ammonium production function, which comprises the following specific steps: step 1, inoculating streptomyces circulans Streptomyces anulatus 255 into an LB bacterial culture medium for culturing; and step 2, re-suspending and adding a protective agent to prepare the microbial inoculum. Further, the temperature in the LB liquid medium in the step 1 is 30 ℃, the culture time is 48 hours, and the concentration of viable bacteria in the microbial inoculum is 2.5X10 8 cfu/mL. Further, the temperature in the LB liquid medium in the step 1 is 30 ℃, the culture time is 48 hours, and the concentration of viable bacteria in the microbial inoculum is 2.5X10 8 cfu/mL. The microbial inoculum provided by the invention has high nitrite tolerance and is applied to nitrite-containing wastewater treatment, nitrogen recovery and ecological restoration. The beneficial effects of the invention are as follows: 1. The strain can be used for the scenes of aquaculture tail water, industrial high-salt wastewater, eutrophication water and the like,