Search

CN-122012300-A - Lactobacillus plantarum strain LES-JC-L-07 and application thereof

CN122012300ACN 122012300 ACN122012300 ACN 122012300ACN-122012300-A

Abstract

The invention provides a selenium-enriched lactobacillus plantarum which is separated from a special food milk fan and successfully stored, the selenium-enriched lactobacillus plantarum has a selenium enrichment rate of 85-91%, the selenium enrichment rate is improved to 93-100% after selenium enrichment condition optimization, the provided lactobacillus plantarum has strong high-concentration sodium selenite resistance, strong acid resistance and strong bile salt resistance, has better tolerance to gastric juice and intestinal juice, and the provided lactobacillus plantarum can be efficiently transformed and utilized to prepare organic selenium, such as preparation of probiotic solid beverage, production of fermented food, processing of milk industry and the like.

Inventors

  • Xia Rao
  • CHEN JIANHUA
  • ZHAO SHIWEI
  • DU YONGXIN
  • ZHANG LIBO
  • XIAO MENGLIN
  • LI DIAOYI
  • ZHANG SHU
  • YIN KERU
  • LI QIONGYING

Assignees

  • 云南来思尔乳业有限公司
  • 大理来思尔乳业有限公司

Dates

Publication Date
20260512
Application Date
20251022

Claims (9)

  1. 1. The lactobacillus plantarum strain LES-JC-L-07 is characterized in that the preservation number of the strain is CGMCC No.35733.
  2. 2. A lactobacillus plantarum strain LES-JC-L-07 according to claim 1, isolated from the special food lactic acid whey of the state of the white autonomous of the university of yunnan.
  3. 3. The lactobacillus plantarum strain LES-JC-L-07 according to claim 1, wherein the selenium enrichment rate of the strain is 85-91%.
  4. 4. The lactobacillus plantarum strain LES-JC-L-07 according to claim 1, wherein the gene sequence of the strain 16S rDNA is shown in a nucleotide or amino acid sequence table.
  5. 5. The lactobacillus plantarum strain LES-JC-L-07 according to claim 1, wherein the lactobacillus plantarum strain LES-JC-L-07 is cultured in a mode of adding sodium selenite with a final concentration of 0.05mg/mL step by step (before, during and after the logarithmic growth phase of the strain) or pre-culturing, the strain is inoculated with 2-10% (bacterial liquid/MRS liquid medium, V/V) and fermented for 20-48 hours, so as to obtain a Gao Fuxi-rate strain, and the Gao Fuxi-rate strain has a selenium enrichment rate of 93-100%.
  6. 6. The Gao Fuxi-rate strain according to claim 5, wherein the radical scavenging rate for DPPH, ABTS and superoxide anions is increased by 7 to 13% as compared with LES-JC-L-07 of a non-selenium-enriched strain (strain obtained after culture in MRS liquid medium).
  7. 7. The lactobacillus plantarum strain LES-JC-L-07 according to claim 1 is characterized in that the viable count of the strain is greater than 10 6 CFU/mL under 1-10 mg/mL of sodium selenite, the survival rate of the strain is 85-93% under acidic conditions, and the survival rate of the strain is 87-93% under bile salts.
  8. 8. Use of the lactobacillus plantarum strain LES-JC-L-07 according to any of claims 1-7 for the production of organic selenium.
  9. 9. Use of lactobacillus plantarum strain LES-JC-L-07 according to claim 8 in organic selenium production, including dairy production, probiotic solid drink production and fermented food production.

Description

Lactobacillus plantarum strain LES-JC-L-07 and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a lactobacillus plantarum strain LES-JC-L-07 and application thereof. Background Selenium has antioxidant, antiinflammatory, antitumor and antiaging effects as essential trace element. About 26 million people worldwide do not meet selenium intake needs in a normal diet, selenium deficiency can cause keshan disease, kaschin-Beck disease and other diseases, and the supplementation of organic selenium can reduce the risk of suffering from the diseases. Selenium mainly comprises 3 forms of inorganic selenium, organic selenium and elemental selenium. Compared with inorganic selenium, simple substance selenium and organic selenium have lower toxicity and are easier to be absorbed by human body. In addition, the simple substance selenium and the organic selenium have higher biological activity and free radical clearance rate, and the content of the simple substance selenium and the organic selenium is positively related to the oxidation resistance of the strain. Milk fans are one of the special foods in the marble region and belong to cheese products. It has unique flavor, and is rich in protein, amino acid and other nutritious components and trace elements essential to human body. The acid whey remaining during the manufacture of the milk fan is rich in lactic acid bacteria resources for which several studies have been conducted by researchers. Lactic acid bacteria are one of the most well known probiotics affecting host health and their role is not only in regulating gastrointestinal balance, but also in antioxidant, anti-inflammatory and anticancer properties. In addition, lactic acid bacteria are also the main species in selenium-rich probiotic research. At present, screening microorganisms with high-efficiency selenium-rich capability to realize large-scale production of organic selenium is one of the key directions of selenium-rich research. Lactobacillus plantarum (Lactiplantibacillus plantarum) is one of the lactic acid bacteria, and previous studies have shown that it has a good effect on selenium enrichment. In recent two years, the research on selenium-enriched probiotics covers the aspects of dairy production, animal cultivation, food fermentation and the like, and is mainly focused on the aspects of screening selenium-enriched probiotics, optimizing fermentation conditions, feed additives and the like. The product produced by converting the lactobacillus plantarum into inorganic selenium has the dual health care effects of probiotics and selenium enrichment, and has wide application prospect in the fields of food, medicine and the like. However, studies have shown that most lactic acid bacteria have less than 85% selenium enrichment and low tolerance to high concentrations of inorganic selenium (e.g., sodium selenite), and that high selenium environments inhibit cell growth and even lead to death. And secondly, the selenium-enriched probiotics have poor stability under the environments of high temperature, strong acid and the like, and seriously influence the application effect of the selenium-enriched probiotics in functional foods. In addition, lactobacillus plantarum resources with high-efficiency selenium conversion capability in nature are scarce, so that the screening and cultivation of strains with high selenium enrichment rate are also the key directions of current researches. Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide the selenium-enriched lactobacillus plantarum which is derived from the strong tolerance of the special food milk fan in the places of the large scale. The technical scheme of the invention is as follows: The invention provides a lactobacillus plantarum strain LES-JC-L-07, and the preservation number of the strain is CGMCC No.35733. The strain is isolated from a special food milk fan in the state of white autonomous in Yunnan province. The strain is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.35733 in the year 8 and 25 of 2025. The invention discloses a preservation description, which is to name lactobacillus plantarum in a classified manner, wherein the Latin name is Lactiplantibacillus plantarum, the biological material of the reference is LES-JC-L-07, the preservation organization is China general microbiological culture Collection center, CGMCC (China general microbiological culture Collection center), the address is North Star Xiya No. 1,3 of the Baiya, beijing, the Chaoyang region, the preservation date is 2025, 8, 25, and the preservation center registration number is CGMCC No.35733. The selenium enrichment rate of the strain is 85-91%. The gene sequence of the strain 16S rDNA is shown in a nucleotide or amino acid sequence table. The invention also provides a method for improv