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CN-122012301-A - Cold-resistant bacillus paradise and application thereof in improving stress resistance of plants

CN122012301ACN 122012301 ACN122012301 ACN 122012301ACN-122012301-A

Abstract

The invention discloses enterobacteria and application thereof in improving stress resistance of plants. The invention relates to the field of biological agriculture, and provides cold-resistant bacillus paradise (Peribacillus frigoritolerans) YJD5-23, which has a registration number of CGMCC No.36069 in the common microorganism center of China Committee for culture Collection of microorganisms. The escherichia coli YJD5-23 provided by the invention can improve the resistance of arabidopsis thaliana, alfalfa, corn and soybean to saline-alkali stress, and is specifically characterized by increasing dry weight, fresh weight, plant length, leaf area and chlorophyll content, improving oxidation resistance, reducing wilt and improving physiological and biochemical indexes. YJD5-23 microbial inoculum improves the property and activity of the saline-alkali soil and improves the abundance and composition of microbial communities. The YJD5-23 strain is found to have the capabilities of secreting IAA, ACC deaminase, phagostimulant, dissolving phosphorus, fixing nitrogen and relieving potassium. The indexes show that the YJD5-23 microbial inoculum has the potential of promoting the growth of bacteria and has important application value in the agricultural field.

Inventors

  • LI LIXIN
  • ZHANG MENGYU
  • WU YUANHANG

Assignees

  • 东北林业大学

Dates

Publication Date
20260512
Application Date
20251024

Claims (10)

  1. 1. The bacillus is characterized in that the bacillus is cold-resistant bacillus parapsilosis (Peribacillus frigoritolerans), the strain number of the bacillus is YJD5-23, and the registration number of the bacillus in the common microorganism center of China Committee for culture Collection of microorganisms is CGMCC No.36069.
  2. 2. The bacillus culture of claim 1, wherein the bacillus culture is obtained by culturing the bacillus of claim 1 in a microbial culture medium.
  3. 3. A microbial inoculum comprising the bacillus of claim 1 or/and the metabolite of the bacillus of claim 1 or/and the culture of claim 2.
  4. 4. A salt and/or alkali stress resistant formulation, characterized in that the salt and/or alkali stress resistant formulation comprises the bacillus of claim 1 or/and the metabolite of the bacillus of claim 1 or/and the culture of claim 2 or/and the microbial inoculum of claim 3.
  5. 5. The use of any of the bacillus of claim 1, the metabolite of bacillus of claim 1, the culture of claim 2, the microbial inoculum of claim 3, the salt and/or alkali stress resistant formulation of claim 4, as follows: The above application may be specifically any of the following: (a1) Enhancing the salt resistance and alkalinity resistance of plants; (a2) Preparing a salt-resistant, alkaline-resistant product of the enhanced plant; (a3) Promoting plant growth; (a4) Preparing a product for promoting plant growth; (a5) Producing IAA; (a6) Preparing an IAA-producing product; (a7) Producing ACC deaminase; (a8) Preparing an ACC deaminase-producing product; (a9) Producing mesophilic ferrite; (a10) Preparation of product producing mesophilic ferrite (A11) Dissolving phosphorus; (a12) Preparing a phosphorus-dissolved product; (a13) Nitrogen fixation; (a14) Preparing a nitrogen fixation product; (a15) A biological membrane; (a16) Preparing a product of the biological film; In the above application, the promotion of plant growth is embodied as all or part of the following: (b1) Promoting the fresh weight of the plant to be increased under the saline-alkali stress condition or non-saline-alkali stress condition; (b2) Promoting plant dry weight increase under saline-alkali stress conditions or non-saline-alkali stress conditions; (b3) Promoting plant root elongation under saline-alkali stress conditions or non-saline-alkali stress conditions; (b4) Promoting plant growth under saline-alkali stress conditions or non-saline-alkali stress conditions; (b5) Promoting the length increase of plant leaves under saline-alkali stress conditions or non-saline-alkali stress conditions; (b6) Promoting plant leaf width increase under saline-alkali stress conditions or non-saline-alkali stress conditions; (b7) Reducing plant leaf wilting under saline-alkali stress conditions or non-saline-alkali stress conditions; (b8) Promoting the chlorophyll content of the plant to be increased under the saline-alkali stress condition or non-saline-alkali stress condition; (b9) Promoting the reduction of the proline content of plants under the saline-alkali stress condition or non-saline-alkali stress condition; (b10) Promoting the reduction of the malondialdehyde content of plants under the saline-alkali stress condition or non-saline-alkali stress condition; (b11) Promoting the improvement of the antioxidant enzyme activity of the plants under the saline-alkali stress condition or non-saline-alkali stress condition; (b12) Regulating plant secondary metabolites under saline-alkali stress conditions or non-saline-alkali stress conditions; (b13) Improving the activity of the saline-alkali soil and the physicochemical property of the saline-alkali soil; (b14) Improving the abundance and composition of the microbial community in the saline-alkali soil.
  6. 6. Use according to claim 5, characterized in that said plants are cruciferous, gramineous and leguminous plants. The plant is a crucifer plant which is a 1), a 2) or a 3) below: a1 A 2), arabidopsis, a 3), arabidopsis; the graminaceous plant may be b 1), b 2) or b 3) below: b1 B 2), rice or maize, b 3), rice or maize; The leguminous plant may be c 1), c 2) or c 3) below: c1 Leguminous family; c 2), alfalfa or soybean, c 3), alfalfa or soybean.
  7. 7. The method for preparing the microbial inoculum according to claim 3, comprising the step of using the bacillus according to claim 1 as an active ingredient to obtain the microbial inoculum.
  8. 8. A bio-organic fertilizer characterized in that the bio-organic fertilizer contains the bacillus of claim 1 or the culture of claim 2 or the microbial inoculum of claim 3.
  9. 9. The method according to claim 5, wherein the plant is a monocotyledonous plant or a dicotyledonous plant.
  10. 10. A method for improving stress resistance or/and promoting plant growth, which comprises treating a plant or a culture medium of a plant with the Bacillus of claim 1, the Bacillus metabolite of claim 1, the culture of claim 2, or the salt and/or alkali stress resistant agent of claim 5.

Description

Cold-resistant bacillus paradise and application thereof in improving stress resistance of plants Technical Field The invention relates to the field of biological agriculture, in particular to bacillus and application thereof in improving stress resistance of plants. Background Plants are subjected to both biotic and abiotic stresses in the natural environment, with saline-alkali stress having become one of the major abiotic stresses threatening the production of sustainable crops worldwide. The artificial activity is superimposed with climate change to continuously aggravate soil salinization, and the excessively high salt concentration causes clay swelling and aggregate disintegration through exchangeable Na+ accumulation and high pH to reduce soil permeability, water holding capacity and permeability, and causes ion imbalance, osmotic stress, reactive Oxygen Species (ROS) explosion, nutrition mobilization blocking and hormone disorder in plants to finally inhibit crop growth and obviously reduce yield. In addition, salinity can also destroy the microbial diversity of the soil and impair the health of the soil. Bacillus sp is widely distributed in rhizosphere and root, is an important member of plant microbiome, has the characteristics of salt tolerance, drought tolerance, acid and alkali resistance, formation of heat-resistant spores and the like, and can survive for a long time in adverse environment and be recovered quickly. A large number of researches show that bacillus can relieve the toxicity of salt stress to plants and improve the salt tolerance and yield of crops through multiple mechanisms of dissolving phosphorus and dissolving potassium, generating plant hormone (such as IAA and cytokinin), inducing System Resistance (ISR), synthesizing antibacterial active substances and the like. The cold-resistant bacillus parapsilosis (Peribacillus frigoritolerans) is one of representative strains of bacillus parapsilosis, and has the dual advantages of low temperature resistance and saline-alkali resistance. The strain can keep high survival rate and metabolic activity under the conditions of low temperature of 0-10 ℃ and pH of 8.5-10.0 and NaCl concentration of more than or equal to 8%, and cold adaptive extracellular polysaccharide, osmotic adjustment compatible solutes (such as proline and betaine) and antioxidant enzyme systems (SOD and CAT) produced by the strain can obviously reduce ROS accumulation induced by salt stress and maintain the osmotic balance and membrane integrity of plant cells. In addition, the cold-resistant bacillus paradoxus can colonize the root surface and root inner cortex of plants, and the adaptability and nutrient acquisition capacity of the plants to saline-alkali environments are enhanced by inducing the expression of host salt-tolerant genes (such as SOS1 and NHX 1), promoting the synthesis of root system auxin and root hair hyperplasia. Therefore, the method for extracting and utilizing the cold-resistant bacillus paradenticola resource has important theoretical and practical significance for realizing green and sustainable agricultural production in the region of double stress of seasonal low temperature and soil salinization. Disclosure of Invention The invention aims to solve the technical problem of how to improve the normal growth of plants in saline-alkali soil, improve the yield and biomass of the plants and improve the stress resistance of the plants. In order to solve the technical problems, the invention firstly screens a strain of cold-resistant bacillus parapsilosis. The cold-resistant bacillus paradenticola provided by the invention is bacillus paradenticola CGMCC No.36069, and the registration number of the bacillus paradenticola in the common microorganism center of China Committee for culture Collection of microorganisms is CGMCC No.36069. The invention also provides a microbial inoculum. The microbial inoculum provided by the invention contains the cold-resistant bacillus paradise and/or the metabolite of the cold-resistant bacillus paradise. In the microbial inoculum, the microbial inoculum contains a carrier in addition to the active ingredient. The carrier may be a carrier commonly used in the pesticide arts and which is biologically inert. The carrier may be a solid carrier or a liquid carrier, the solid carrier may be a mineral material, a plant material or a high molecular compound, the mineral material may be at least one of clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica and diatomaceous earth, the plant material may be at least one of corn flour, bean flour and starch, the high molecular compound may be polyvinyl alcohol and/or polyglycol, the liquid carrier may be an organic solvent, vegetable oil, mineral oil or water, and the organic solvent may be decane and/or dodecane. Among the above-mentioned bacterial agents, the formulation of the bacterial agent can be various formulations, such as liquid, emulsion, suspending agent