CN-122012305-A - Pseudomonas putida (Pseudomonas putida) RT12 and application thereof

Abstract

The invention relates to a new saccharomycete, in particular to pseudomonas putida (Pseudomonas putida) RT12 and application thereof in degradation of juglone in walnut green seedcase. The strain is preserved in China general microbiological culture collection center (CGMCC) at the date of 11 and 19 of 2025, and the preservation address is CGMCC No.36686 of China academy of sciences of China, no.3, west-Lu No. 1, games area North Star, beijing, and the market. The Pseudomonas putida (Pseudomonas putida) RT12 is obtained by separation, screening and identification from the region of the Akesu red flag slope of Xinjiang, and the growth characteristics, physiological and biochemical properties, ester production capacity and tolerance experiments of the strain are verified, so that the strain has the activity of efficiently degrading juglone, and the Pseudomonas putida (Pseudomonas putida) RT12 has a good application prospect and can be widely applied to the agricultural solid waste resource treatment industry.

Inventors

• ZHOU ZEYING
• ZHANG QIANG
• HUANG MINMIN
• NIU BEN
• NING WANJUN
• WU DI
• Mahmudi Abu-lai
• WANG BAOQING
• Adi, come on Maimaiti

Assignees

• 新疆林科院经济林研究所

Dates

Publication Date

20260512

Application Date

20251215

Claims (8)

1. 1. Pseudomonas putida (Pseudomonas putida) RT12 is characterized in that the strain is preserved in China general microbiological culture collection center (CGMCC) with a preservation address of CGMCC No.3 of China academy of sciences of North Star West way No. 1 in the Korean area of Beijing at the year 2025, 11 and 19, and a preservation number of CGMCC No.36686.
2. 2. The pseudomonas putida (Pseudomonas putida) RT12 of claim 1, wherein the 16S rRNA gene sequence is set forth in SEQ ID No. 1.
3. 3. An isolated culture medium of pseudomonas putida (Pseudomonas putida) RT12, which is characterized in that the isolated culture medium adopts a PDA culture medium or a juglone screening culture medium.
4. 4. The isolated culture medium of Pseudomonas putida (Pseudomonas putida) RT12 according to claim 3, wherein the juglone culture medium is formulated as 750. Mu.g/L potassium dihydrogen phosphate, 480. Mu.g/L disodium hydrogen phosphate, 40. Mu.g/L ammonium chloride, 10. Mu.g/L magnesium sulfate, 10. Mu.g/L calcium chloride, 0.2. Mu.g/L ferric chloride, 15. Mu.g/L juglone.
5. 5. The microbial agent is characterized by comprising pseudomonas putida (Pseudomonas putida) RT12 as claimed in claim 1, and a fermentation culture thereof, which can be a liquid microbial agent or a solid microbial agent, and can be prepared by adopting a conventional technical means and adding a carrier or other auxiliary materials allowed in the field of microbial agents.
6. 6. The walnut green seedcase starter is characterized in that the starter comprises Pseudomonas putida (Pseudomonas putida) RT12 as described in claim 1, and a fermentation culture thereof, which can be liquid microbial agent or solid microbial agent, and can be prepared by adding carriers or other auxiliary materials allowed in the field of microbial preparations by adopting conventional technical means.
7. 7. The preparation for degrading juglone is characterized in that the preparation comprises Pseudomonas putida (Pseudomonas putida) RT12 as claimed in claim 1, and a fermentation culture thereof, which can be a liquid microbial agent or a solid microbial agent, and can be prepared by adopting a conventional technical means and adding a carrier or other auxiliary materials allowed in the field of microbial preparations.
8. 8. Use of pseudomonas putida (Pseudomonas putida) RT12, a fermentation product thereof, or a zymophyte agent according to claim 1 in a juglone degrading fermentation.

Description

Pseudomonas putida (Pseudomonas putida) RT12 and application thereof Technical Field The invention relates to the technical field of microbial strains and application thereof, in particular to pseudomonas putida new strain (Pseudomonas putida) RT12 and application thereof in agricultural solid waste resource treatment. Background The green peel of the walnut (namely, the green peel of the outer layer of the walnut fruit) is used as a main byproduct of the primary processing of the walnut, and the problem of harmless treatment becomes one of key challenges for restricting the sustainable development of the walnut industry. The annual output of the green tangerine peel of the walnut is huge, and the annual output of the green tangerine peel waste in the Xinjiang area of China exceeds 578 ten thousand tons. The traditional treatment mode is mainly to discard at will, and walnut quinone and other allelochemicals contained in green tangerine peel release toxic components in the natural decay process, pollute soil and water, even inhibit crop growth or cause fish fry death, and the problems are particularly serious in walnut producing areas such as Xinjiang Hetian, keshen, ackersu and the like. Juglone (Juglone, 5-hydroxy-1, 4-naphthoquinone) is a natural quinone compound widely existing in Juglandaceae plants (Juglandaceae), and is one of important secondary metabolites of plants such as black walnut (Juglans nigra), white walnut (Juglans cinerea) and walnut (Juglans regia). It is stored in the plant body mainly in a glucoside-bound form, and when plant tissues are damaged or decomposed, juglone is released in a free state into the surrounding environment. Juglone has strong chemical activity and biotoxicity, can generate oxidation-reduction reaction with various cell components, has obvious inhibition effect on plants, microorganisms and animal cells, and is a typical chemosensory substance (allelopathic compound). Juglone plays a role of a plant competing factor in nature, and can help juglandaceae plants form a chemosensory zone (allelopathic zone) by inhibiting germination and root growth of surrounding plant seeds. However, the ecological significance of this allelopathy presents new problems in the artificial environment. Along with the expansion of walnut planting area, wood processing, stacking of walnut shells and leaves and production of industrial extracts, juglone gradually accumulates in the agricultural ecosystem and the surrounding environment. Juglone has certain durability and fat solubility, is easy to be adsorbed on the surfaces of soil organic matters and particles, is not easy to be naturally degraded, and can cause soil microecological imbalance, microbial diversity reduction and plant growth disorder in local areas. For the water body, juglone can enter surface water or underground water through runoff or infiltration, and has potential toxic effect on aquatic organisms. Therefore, juglone is not only plant source ecological toxin, but also pollution factor to be solved in some regional environment restoration. The current treatments for juglone contamination are mainly focused on physical and chemical methods. The physical adsorption method can reduce the concentration of juglone to a certain extent by adsorbing the juglone by using activated carbon, bentonite, biochar and other materials, but has limited adsorption capacity, and the regeneration process of the adsorption material is complex, so that secondary pollution is easy to generate. The chemical oxidation method utilizes strong oxidants such as ozone, persulfates, fenton reagent and the like to decompose juglone, and has the advantages of high degradation rate, severe process conditions, high cost and possibility of generating intermediate products with unknown toxicity. In addition, the photocatalytic degradation technology (such as TiO 2 photocatalysis) has a certain removal effect on juglone, but the efficiency is obviously reduced in an environment with lower natural illumination intensity or turbid medium, so that the large-scale application is difficult to realize. Therefore, the existing physicochemical method has obvious defects in the aspects of cost, operation condition, environmental compatibility and secondary pollution control. In contrast, the biodegradation method has the advantages of environmental friendliness, low energy consumption, thorough treatment and the like, and is a hot spot direction of research in recent years. Part of microorganisms in natural environment have metabolic conversion capability on quinone compounds, and can be converted into low-toxicity or nontoxic products through oxidation-reduction, hydroxylation, decarboxylation and other reactions. Some fungi (such as white rot fungi, aspergillus niger, etc.) and bacteria (such as Bacillus, rhodococcus genus) have been shown to degrade naphthoquinone compounds. However, specific efficient degradation strains aiming at juglone are still freshly repo