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CN-122012308-A - Brevundimonas strain and application thereof in degradation of dinotefuran and improvement of resistance

CN122012308ACN 122012308 ACN122012308 ACN 122012308ACN-122012308-A

Abstract

The invention relates to an agricultural biological control technology, and aims to provide a Brevundimonas strain and application thereof in degradation of dinotefuran and improvement of resistance. The Brevundimonas strain is preserved in China center for type culture collection, and has the preservation name of Brevundimonas D-1, the Latin name of Brevundimonas huaxiensis and the preservation number of CCTCC NO: M20252463. The 16S rRNA gene sequence of the strain is shown as SEQ ID NO. 1. The strain has novel source, has functional specificity and pertinence, shows remarkable degradation capability on dinotefuran, can improve the inherent resistance of parasitic wasp on dinotefuran by supplementing the strain, utilizes microorganisms for degradation and bioaugmentation, avoids secondary pollution, and accords with the development direction of modern agriculture.

Inventors

  • HUANG JIANHUA
  • Fu Lidi
  • SHI WENQI
  • WANG YING
  • CHEN JIANI

Assignees

  • 浙江大学

Dates

Publication Date
20260512
Application Date
20260109
Priority Date
20260108

Claims (10)

  1. 1. The strain is characterized in that the strain is preserved in China Center for Type Culture Collection (CCTCC) with the preservation name of Brevundimonas D-1, the Latin classification of Brevundimonas huaxiensis and the preservation number of CCTCC NO: M20252463.
  2. 2. The strain of shortwave monad according to claim 1, wherein the strain is isolated from intestinal contents of fruit fly ringworm bees adults.
  3. 3. The Brevundimonas strain of claim 1, wherein the 16S rRNA gene sequence of the strain is shown in SEQ ID NO. 1.
  4. 4. A method for culturing a Brevundimonas strain according to any one of claims 1 to 3, wherein the strain is transferred to a liquid phase medium, and cultured under 28-32 ℃ and shaking conditions, wherein the liquid phase medium is LB medium or inorganic salt medium with dinotefuran as the only carbon source.
  5. 5. Use of a strain of shortwave uniconas as claimed in any one of claims 1 to 3 for degrading dinotefuran or for increasing the resistance of parasitic wasps to dinotefuran.
  6. 6. A microbial agent having a function of degrading dinotefuran, characterized in that it comprises the shortwave monad strain of any one of claims 1 to 3 as an active ingredient, and an agriculturally acceptable carrier.
  7. 7. The use method of the microbial agent is characterized in that the microbial agent is added into water according to the dosage proportion of 1% -5% and uniformly mixed when in use, then the microbial agent is applied to fields or forests polluted by dinotefuran in a spraying mode, the application dosage is controlled within the range of 450-750L/hm 2 , the carrier in the microbial agent is sterile phosphate buffer, and the concentration of viable bacteria is 2X 10 9 cfu/mL.
  8. 8. A microbial agent having a function of improving the resistance of parasitic wasps to dinotefuran, characterized in that the microbial agent comprises the shortwave monad strain of any one of claims 1 to 3 as an active ingredient, and an acceptable carrier in parasitic wasp feed.
  9. 9. The method for using the microbial agent of claim 8, wherein the parasitic wasps are fed with a sucrose aqueous solution containing antibiotics for 1 day before use, the bacterial colony in the intestinal tracts of the parasitic wasps is removed, then the microbial agent is fed for 2 days, and the carrier in the microbial agent is sucrose water with the living bacterial concentration of 2X 10 9 cfu/mL.
  10. 10. The method of claim 9, wherein the parasitic wasp is a parasitic wasp of genus goiter or genus goiter.

Description

Brevundimonas strain and application thereof in degradation of dinotefuran and improvement of resistance Technical Field The invention belongs to the technical field of crossing of an environmental microorganism technology and agricultural biological control, and particularly relates to novel shortwave monad with a pesticide degradation function and application of the strain in degradation of anabasine pesticide dinotefuran. Background Neonicotinoid insecticides are one of the most widely used insecticide worldwide, however, their high water solubility and durability also pose a serious threat to the ecological environment and non-target organisms (especially pollinating and natural enemy insects). The first two generations of pesticides such as imidacloprid and thiamethoxam are forbidden by various countries and organizations such as European Union in 2020, and dinotefuran is used as a new generation of neonicotinoid pesticide, has different chemical structures and is safer for mammals, so that the application of the novel neonicotinoid pesticide is increasingly wide, but the degradation behavior and the ecological toxicity of dinotefuran in the environment are greatly different due to the different chemical structures. At present, microbial remediation technology for pesticide residues is considered as a green, sustainable solution. Some microorganisms have been reported to degrade other neonicotinoid insecticides, for example, patent CN112779194B discloses a strain of gordonia alcaligenes for degrading imidacloprid and patent CN116376764A discloses a strain of pseudomonas for degrading acetamiprid. However, microbial resources, particularly functional strains derived from the intestinal tract of natural enemy insects, having high efficiency and specific degradability to dinotefuran are rarely reported at present. In nature, the fruit flies are widely distributed, the number is huge, the variety is great, and in agricultural production, especially in berry fruit planting industry, huge harm is caused, so that huge economic loss is caused. It is counted that about 90% of fruit flies are parasitized by parasitic wasps in nature. It has now been found and reported that Drosophila parasitic wasps are of the genus 16 of the 4 families, wherein the number of three species of the genus Cyclopentablaceae FIGITIDAE key-chest gall wasps of the subfamily Eucoilinae, the genus Leptopilina, the genus Ganaspis and the genus Asobara of the genus Braconidae open arm cocoon of the family Apiaceae is in excess of 20%. The small ring gall bees and the open arm cocoon bees are dominant parasitic natural enemies of fruit fly pests, and the small ring gall bees and the open arm cocoon bees show excellent biological control efficiency in ecological systems such as orchards, namely the small ring gall bees (L. drosophilae) of fruit flies can accurately position and parasitic early larvae of various fruit flies, the population base number of the small ring gall bees is effectively restrained, and the Japanese open arm anti-jaw cocoon bees (A.japonica) are good at parasitizing middle and later larvae, and have high parasitizing rate and remarkable pest control effect. However, the application of field pesticides severely affects the population survival and pest control efficacy of parasitic bees, which themselves often die in large amounts due to exposure to the pesticide environment, resulting in failure of control. In recent years, research shows that insect intestinal symbiotic bacteria possibly participate in the detoxification and metabolism process of a host on pesticides, so that searching for a microbial resource capable of specifically degrading a specific pesticide and protecting natural enemy insects is important for realizing sustainable development of agriculture. Disclosure of Invention The invention aims to solve the technical problem of overcoming the defects of the prior art and providing a Brevundimonas strain and application thereof in dinotefuran degradation. To achieve the above object, the solution of the present invention is: a Brevundimonas strain is provided, and is preserved in China center for type culture collection, wherein the preservation name is Brevundimonas D-1, the Latin classification is Brevundimonas huaxiensis, and the preservation number is CCTCC NO: M20252463. As a preferred embodiment of the present invention, the Brevundimonas strain is isolated from the intestinal contents of fruit fly ringworm gall bees adults. As a preferable scheme of the invention, the 16S rRNA gene sequence of the Brevundimonas strain is shown as SEQ ID NO. 1. The invention also provides a culture method of the shortwave monad strain, which comprises the steps of transferring the strain into a liquid phase culture medium, and culturing under 28-32 ℃ and shaking conditions, wherein the liquid phase culture medium is an LB culture medium or an inorganic salt culture medium with dinotefuran as a unique carbon source. Th