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CN-122012310-A - Sphingomonas Sphingopyxis sp, R3-92 and application thereof

CN122012310ACN 122012310 ACN122012310 ACN 122012310ACN-122012310-A

Abstract

The invention relates to the technical field of microbiology and biological nano-selenium preparation, and provides a sphingosine box fungus Sphingopyxis sp.R3-92 and application thereof. The strain R3-92 is a new species of sphingosine box bacteria Sphingopyxis sp which can efficiently convert selenite into nano-selenium, and is named as selenium reduction sphingosine box bacteria (Sphingopyxis selenitireducens) with the preservation number of GDMCC No:63729. The strain R3-92 has stronger tolerance to selenate and selenite, and a plate culture test shows that the strain R3-92 can tolerate 100 mM selenate and 80 mM selenite. The invention also establishes a method for preparing a large amount of nano-selenium by utilizing R3-92 in a bioreactor, and has obvious application effects in fertilizer, feed, selenium-rich functional food processing, health care products and medical products.

Inventors

  • GUO YANBIN
  • LIU YI
  • ZHANG YAFENG
  • YAO ZHEN
  • ZHANG SASA
  • LI KUI
  • DAI LU
  • HE LIANZHEN

Assignees

  • 中国农业大学
  • 青海省第五地质勘查院

Dates

Publication Date
20260512
Application Date
20260109

Claims (9)

  1. 1. Sphingomonas Sphingopyxis sp.R 3-92, accession number GDMCC No:63729.
  2. 2. A microbial preparation comprising the sphingosine according to claim 1.
  3. 3. The use of the sphingosine box fungus according to claim 1 in selenium polluted environment restoration and nano-selenium preparation.
  4. 4. The nano-selenium biosynthesis method is characterized in that the sphingosine box bacterium according to claim 1 is inoculated into a culture medium added with selenite, and after a period of culture, nano-selenium is separated and purified from the culture solution.
  5. 5. The method of claim 4, wherein the initial selenium concentration in the selenite-supplemented medium is from 2 to 80 mM.
  6. 6. The method of claim 5, wherein the initial selenium concentration in the selenite-supplemented medium is 2-20 mM.
  7. 7. The method of claim 6, wherein the initial selenium concentration in the selenite-supplemented medium is 5 mM.
  8. 8. The method according to any one of claims 4 to 7, wherein the basic components of the culture medium are yeast extract 5 g/L, tryptone 10 g/L, sodium chloride 10 g/L, solid medium supplemented with agar 18 g/L, pH7.0-7.2.
  9. 9. The method according to any one of claims 4 to 7, wherein the culturing conditions are 28.+ -. 0.5 ℃ and the rotational speed is 150 rpm.

Description

Sphingomonas Sphingopyxis sp, R3-92 and application thereof Technical Field The invention relates to the technical field of microbiology and biological nano-selenium preparation, in particular to a sphingosine box fungus Sphingopyxis sp.R3-92 and application thereof. Background Selenium is an element commonly found in nature, and has the main valence states of negative divalent, zero-valent, tetravalent and hexavalent. Since the industry society, glass manufacturing industry uses selenium for coloring and decoloring, metallurgical industry uses selenium dioxide for electrolyzing manganese, and selenium dust and selenium-containing waste materials are generated in the mining process. In addition, selenium is used as a trace element necessary for people and animals, and has important application in the fields of health care products, feeds, fertilizers and the like. The high selenium wastewater produced by these industrial activities contains mainly selenate [ Se (VI) ] and selenite [ Se (IV) ], which are two highly toxic inorganic selenium compounds. Selenium poisoning can be caused when high concentration selenium is ingested by human body, which leads to nail and hair loss, inappetence, reduced reproductive function and reduced immunity. In contrast, nano selenium is a bacteriostatic agent with low toxicity and high biological activity, and has great application potential in the biomedical field and the fields of food and feed additives. Therefore, the method for restoring the selenium polluted wastewater is low in cost, green and efficient. Sphingomonas (Sphingopyxis) belongs to the family Sphingomonasceae (Sphingomonadaceae) and is widely distributed in soil, water and plant rhizosphere. The genus has 22 formally and effectively published species. The sphingosine box fungus has great potential for degrading various environmental pollutants, such as microcystin, cyhalofop-butyl, chlorpyrifos and the like, has the capability of adsorbing cadmium and manganese in water, and is an important strain resource [1] for biological repair. There is no report of biological conversion of selenium compounds by Sphingomonas. Disclosure of Invention The invention aims to provide sphingosine box bacteria Sphingopyxis sp, R3-92 capable of efficiently converting selenite and/or selenate into nano-selenium and application thereof. In order to achieve the purpose of the invention, in a first aspect, the invention provides a strain R3-92 which is separated and purified from selenium-rich soil of a pond dam of Enshi fish in Hubei and can reduce selenite to generate nano selenium, which is classified and named Sphingopyxis sp, and is preserved in the microorganism strain collection (GDMCC) of Guangdong province, address: building 5 of No. 59 of Dai 100 in Guangzhou city martyr of Guangdong province, institute of microbiology, post code 510070, preservation number GDMCC No:63729, and preservation date 2023, 8 months and 11 days. In a second aspect, the present invention provides a microbial preparation comprising Sphingomonas R3-92. In a third aspect, the invention provides application of Sphingomonas R3-92 in selenium polluted environment restoration and nano-selenium preparation. In a fourth aspect, the invention provides a nano-selenium biosynthesis method, which comprises inoculating Sphingomonas R3-92 into a culture medium added with selenite, culturing for a period of time, and separating and purifying nano-selenium from the culture solution. The initial selenium concentration in the selenite-added medium is 2-80 mM, preferably 2-20 mM, more preferably 5 mM. In one embodiment of the invention, the basic components of the culture medium are yeast extract 5g/L, tryptone 10g/L, sodium chloride 10g/L, and agar 18 g/L, pH7.0-7.2 is added to the solid culture medium. Preferably, the culture conditions are 28+ -0.5℃and the rotational speed is 150 rpm. By means of the technical scheme, the invention has at least the following advantages and beneficial effects: The sphingosine box bacteria R3-92 of the invention has stronger tolerance to selenate and selenite, and a flat plate test shows that R3-92 can tolerate 100 mM selenate and 80mM selenite. The invention also establishes a method for preparing a large amount of nano-selenium by utilizing R3-92 in a bioreactor, which can be used for bioremediation of selenium-containing wastewater and synthesis of biological nano-selenium and has wide application prospect. Drawings FIG. 1 shows colony and fungus body morphology characteristics of the strain R3-92 of the present invention. FIG. 2 is a 16S rRNA gene evolutionary tree of strain R3-92 of the present invention. Note that Bootstrap values greater than 50% are shown. In parentheses is GenBank accession number of the strain 16S rRNA gene sequence, and the scale indicates 1% nucleotide substitution rate. FIG. 3 is a phylogenetic tree constructed by using UBCG based on 92 core genes of the strain R3-92 of the present invention. Note that