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CN-122012312-A - Bacterial cellulose strain with high yield and application thereof

CN122012312ACN 122012312 ACN122012312 ACN 122012312ACN-122012312-A

Abstract

The invention relates to the technical field of microorganisms, and discloses a high-yield bacterial cellulose strain and application thereof, and provides a high-yield bacterial cellulose strain, namely Komagataeibacter nataicola JF11, which is preserved in the Guangdong province microorganism strain preservation center, wherein the preservation number is GDMCC NO:67317, and the preservation date is 2025, 11 and 18. The bacterial cellulose strain with high yield provided by the invention can efficiently produce bacterial cellulose, and has the advantages of high yield, simple culture process and low production cost.

Inventors

  • LIU SIXIN
  • YANG QIN
  • LIN XUE
  • GUO YAQI
  • WANG TONG
  • LI CONGFA

Assignees

  • 海南大学

Dates

Publication Date
20260512
Application Date
20260114

Claims (10)

  1. 1. The high-yield bacterial cellulose strain is Komagataeibacter nataicola JF, and is deposited in the Guangdong province microorganism strain collection center, wherein the deposition number is GDMCC NO:67317, and the deposition date is 2025, 11 and 18.
  2. 2. A microbial agent comprising the high-yielding bacterial cellulose strain of claim 1.
  3. 3. A composition comprising, as a main ingredient, the composition comprising the high yielding bacterial cellulose strain of claim 1.
  4. 4. Use of a high-yielding bacterial cellulose strain according to claim 1, wherein the use is in the production of bacterial cellulose.
  5. 5. Use of the microbial agent of claim 2, in the manufacture of bacterial cellulose.
  6. 6. Use of a composition according to claim 3, in the manufacture of bacterial cellulose.
  7. 7. A method of producing bacterial cellulose comprising the steps of: (1) Preparing a culture medium, namely preparing a pre-Fermented Coconut Water (FCW) liquid culture medium, wherein the formula of the pre-Fermented Coconut Water (FCW) liquid culture medium is (NH 4 ) 2 SO 4 3g/L、MgSO 4 ・7H 2 O 0.3g/L、KH 2 PO 4 0.3g/L、, the pre-fermented coconut water is 70%, the sugar degree is adjusted to 6 Brix, the pH is 4.5, and the culture medium is sterilized at 115 ℃ for 25min; (2) Inoculating and culturing, namely inoculating a high-yield bacterial cellulose strain into the culture medium for culturing; (3) Harvesting and purifying, namely collecting bacterial cellulose membranes after the stationary culture is finished, washing, and drying to constant weight at 70 ℃ to obtain the bacterial cellulose; Wherein the high-yielding bacterial cellulose strain is the high-yielding bacterial cellulose strain according to claim 1.
  8. 8. The method for producing bacterial cellulose according to claim 7, wherein said high-producing bacterial cellulose strain in step (2) is inoculated into said medium at a volume ratio of 2%.
  9. 9. The method for producing bacterial cellulose according to claim 7, wherein the washing is specifically deionized water washing, treatment in a 75 ℃ water bath with 0.1mol/L NaOH solution for 2 hours, soaking in 0.5% acetic acid solution for 1 day, and rinsing with clear water.
  10. 10. The method for producing bacterial cellulose according to claim 7, wherein said culturing in step (2) is specifically stationary culturing at 30℃for 4 to 8 days.

Description

Bacterial cellulose strain with high yield and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a high-yield bacterial cellulose strain and application thereof. Background Bacterial cellulose (Bacterial cellulose, BC) is a natural high molecular polymer synthesized by microorganisms, has unique properties of high purity, high crystallinity, high water retention, good biocompatibility, degradability and the like, and has wide application prospects in the fields of foods (such as coconut), medicines (such as artificial skin and drug carriers), cosmetics, advanced materials and the like. At present, the main bacterial strain for industrial production of bacterial cellulose is acetobacter xylinum. However, acetobacter xylinum has some inherent disadvantages such as (1) limited yield of bacterial cellulose, resulting in high production cost, (2) sensitivity to fermentation environment (e.g. pH, dissolved oxygen, inhibitor), unstable fermentation process, and (3) the structure and performance of the produced bacterial cellulose are insufficient in some specific applications (e.g. high-strength materials). Based on the above, a new strain capable of realizing stable and efficient BC synthesis in a low-cost pre-Fermented Coconut Water (FCW) liquid culture medium is provided, and is important to promote low-cost and high-performance application of bacterial cellulose. Disclosure of Invention In view of the above, the present invention provides a bacterial cellulose strain with high yield and application thereof, which aims to solve at least one of the problems of the prior art. The invention provides a high-yield bacterial cellulose strain which is Komagataeibacter nataicola JF and is preserved in the Guangdong province microorganism strain preservation center, wherein the preservation number is GDMCC NO:67317, and the preservation date is 2025, 11 and 18. The invention also provides a microbial inoculum, which comprises the high-yield bacterial cellulose strain according to the technical scheme. The invention also provides a composition, which comprises the high-yield bacterial cellulose strain according to the technical scheme. The invention also provides application of the high-yield bacterial cellulose strain in the technical scheme, and the application is application in bacterial cellulose production. The invention also provides application of the microbial inoculum according to the technical scheme, wherein the application is application in production of bacterial cellulose. The invention also provides an application of the composition in the technical scheme, which is characterized in that the application is an application in the production of bacterial cellulose. The invention also provides a method for producing bacterial cellulose, comprising the following steps: (1) Preparing a culture medium, namely preparing a pre-fermented coconut water liquid culture medium, wherein the formula of the pre-Fermented Coconut Water (FCW) liquid culture medium is (NH4)2SO4 3g/L、MgSO4・7H2O 0.3g/L、KH2PO4 0.3g/L、, the pre-fermented coconut water is 70%, the sugar degree is adjusted to 6 Brix, the pH is 4.5, and the culture medium is sterilized for 25min at the temperature of 115 ℃; (2) Inoculating and culturing, namely inoculating a high-yield bacterial cellulose strain into the culture medium for culturing; (3) Harvesting and purifying, namely collecting bacterial cellulose membranes after the stationary culture is finished, washing, and drying to constant weight at 70 ℃ to obtain the bacterial cellulose; Wherein the high-yielding bacterial cellulose strain is the high-yielding bacterial cellulose strain according to claim 1. Preferably, the high-yielding bacterial cellulose strain in step (2) is inoculated into the medium at a volume ratio of 2%. Preferably, the washing is specifically carried out by adopting deionized water for washing, adopting 0.1mol/L NaOH solution for 75 ℃ water bath treatment for 2 hours, adopting 0.5% acetic acid solution for soaking for 1 day, and adopting clear water for flushing. Preferably, the culture in the step (2) is specifically stationary culture at 30 ℃ for 4-8 days. Compared with the prior art, the invention has the beneficial effects that: The invention provides a high-yield bacterial cellulose strain, namely Komagataeibacter nataicola JF11, which is deposited in the microorganism strain collection of Guangdong province, wherein the deposition number is GDMCC NO:67317, and the deposition date is 2025, 11, 18 days. The bacterial cellulose strain with high yield provided by the invention can efficiently produce bacterial cellulose, and has the advantages of high yield, simple culture process and low production cost. Drawings Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for