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CN-122012313-A - Pseudomonas strain JC1303 and application thereof

CN122012313ACN 122012313 ACN122012313 ACN 122012313ACN-122012313-A

Abstract

The invention relates to the technical field of novel bacterial strains for degrading cellulose, in particular to a pseudomonas bacterial strain JC1303 and application thereof. Through whole genome sequencing and functional annotation, the first system reveals that the strain carries a complete cellulose degradation enzyme system including endo beta-1, 4-glucanase, cellulase and beta-glucosidase, has a plurality of central metabolic pathways supporting efficient degradation and utilization of cellulose, and determines specific gene resources through general genome analysis. The strain has the remarkable technical effects that the strain shows higher cellulase activity on the 5 th day of culture, and can stably and efficiently degrade cellulose materials such as agricultural wastes in a salt-containing environment by virtue of the unique genetic background and marine source characteristics.

Inventors

  • WANG FENGLIN
  • ZHANG XIUMEI
  • HE YAODONG
  • PENG ZHENGWEI

Assignees

  • 浙江海洋大学

Dates

Publication Date
20260512
Application Date
20260106

Claims (8)

  1. 1. A Pseudomonas strain JC1303 is characterized in that the Pseudomonas strain JC1303 is preserved in China center for type culture Collection with the preservation number of M20252026 and the classification of Pseudoxanthomonas sp.JC1303.
  2. 2. A microbial preparation comprising the pseudomonas strain JC1303 according to claim 1, together with one or more agriculturally acceptable carriers.
  3. 3. The microbial preparation according to claim 2, wherein the preparation is a lyophilized powder or liquid microbial preparation, and the viable count of the strain JC1303 is not less than 1 x 10CFU/g.
  4. 4. A method of degrading a cellulosic material comprising the step of contacting the cellulosic material with the pseudomonas strain JC1303 of claim 1, or the microbial agent of claim 2 or 3.
  5. 5. The method of claim 4, wherein the cellulosic material is derived from agricultural waste.
  6. 6. The method according to claim 4, wherein the culture is carried out in a medium containing sodium carboxymethylcellulose or microcrystalline cellulose at 25℃to 30℃and 150 to 200rpm for 3 to 7 days.
  7. 7. A method for producing reducing sugars, characterized in that a cellulosic material is degraded by the method according to any one of claims 4-6 and the produced reducing sugars comprising glucose are collected.
  8. 8. Use of the strain JC1303 and/or metabolites thereof according to claim 1 for the preparation of a formulation for degrading cellulose, treating agricultural waste or producing biofuel.

Description

Pseudomonas strain JC1303 and application thereof Technical Field The invention relates to the technical field of novel bacterial strains for degrading cellulose, in particular to a pseudomonas bacterial strain JC1303 and application thereof. Background Cellulose is a linear polysaccharide formed by glucose units connected through beta-1, 4-glycosidic bonds, is a main component of plant cell walls and is recognized as the most abundant organic compound and important renewable resources on the earth. Cellulose is useful in the production of alcohols, gaseous fuels, and degradable materials. However, due to its complex chemical structure, efficient utilization presents a major challenge, which is particularly pronounced in agricultural waste treatment-cellulose's resistance to degradation reduces the recycling and composting efficiency. In China, a large amount of agricultural organic waste is directly burned or piled up, which causes resource waste and environmental pollution. Therefore, developing efficient cellulose resource utilization technology has important significance for relieving resource shortage and promoting environmental protection. Microbial cellulase plays a central role in cellulose degradation, which is important for solving the waste of cellulose resources. The cellulase mainly comprises endo beta-1, 4-glucanase and beta-1, 4-glucosidase, and can decompose cellulose into glucose monomers. Therefore, the screening of cellulose degrading strains has practical significance for the development and utilization of cellulose resources. The marine ecological system has abundant microbial resources and highly diversified metabolic capabilities, and microorganisms living in the marine extreme environment can generate cellulase with excellent properties such as salt resistance, acid resistance, alkali resistance, pressure resistance and the like, so that the marine ecological system provides important advantages for development and application of the cellulase. Disclosure of Invention The invention aims to overcome the defects of the prior art, provides a novel marine pseudomonas strain JC1303 with a preservation number of CCTCC NO: M20252026, and discloses a complete cellulose degradation enzyme system comprising endo beta-1, 4-glucanase, cellulase and beta-glucosidase for the first time through whole genome sequencing and functional annotation, wherein the strain carries a complete cellulose degradation enzyme system comprising endo beta-1, 4-glucanase, cellulase and beta-glucosidase, has a plurality of central metabolic pathways supporting efficient degradation and utilization of cellulose, and defines specific gene resources through genome analysis. The strain shows higher cellulase activity (for example FPase activity reaches 0.65+/-0.11U/mL) on the 5 th day of culture, and can stably and efficiently degrade cellulose materials such as agricultural wastes and the like in a salt-containing environment. The first aspect of the invention provides a Pseudomonas strain JC1303,2025 which is preserved in China Center for Type Culture Collection (CCTCC) No. M20252026 and classified and named Pseudoxanthomonas sp.JC1303. Through detection, the 16S rRNA gene sequence of the Pseudomonas strain JC1303 is shown as SEQ ID NO. 1, and the whole genome sequence is shown as SEQ ID NO. 2. The second aspect of the present invention provides a microbial preparation comprising the above-described Pseudomonas strain JC1303, and one or more agriculturally acceptable carriers. Further, the preparation is in the form of freeze-dried powder or liquid microbial inoculum, and the viable count of the strain JC1303 is not less than 1X 10CFU/g. In a third aspect the present invention provides a method of degrading cellulosic material comprising the step of contacting cellulosic material with a Pseudomonas strain JC1303 as described above, or a microbial preparation as described above. Further, the cellulosic material is derived from agricultural waste. Further, in a medium containing sodium carboxymethyl cellulose or microcrystalline cellulose, the culture is carried out at 25-30℃and 150-200rpm for 3-7 days. In a fourth aspect the invention provides a method of producing a reducing sugar, degrading cellulosic material using the method described above and collecting the produced reducing sugar comprising glucose. In a fifth aspect the present invention provides the use of the strain JC1303 and/or metabolites thereof described above in the preparation of a formulation for degrading cellulose, treating agricultural waste or producing biofuel. Compared with the prior art, the invention has the beneficial effects that: The application provides a novel marine pseudomonas strain JC1303 with a preservation number of CCTCC NO: M20252026, and provides a microbial preparation containing the strain, a method for degrading cellulose materials and producing reducing sugar. Through whole genome sequencing and functional annotation, the first