CN-122012315-A - Application of bacillus bailii E1 in improving rice bacterial leaf blight resistance

Abstract

The invention belongs to the technical field of biological control and provides application of bacillus subtilis E1 in improving bacterial leaf blight resistance of rice, wherein bacterial liquid prepared by the bacillus subtilis E1 is used for spraying on the surfaces of four-leaf stage rice leaves, and the treatment effect of the bacillus subtilis E1 on bacterial leaf blight of rice is evaluated, and results show that the bacillus subtilis E1 can obviously inhibit bacterial leaf blight growth, improve bacterial leaf blight resistance, solve the problems of low colonization rate and unstable effect of the traditional biological control bacteria, replace or reduce chemical bactericides and provide theoretical basis for bacterial agent development.

Inventors

• ZHANG DEYONG
• KANG HOUXIANG
• SU PIN
• PENG WEIYE
• Peng Qianze
• GAO LIMING

Assignees

• 岳麓山实验室

Dates

Publication Date

20260512

Application Date

20260120

Claims (7)

1. 1. The bacillus beleiensis E1 is characterized in that bacillus beleiensis E1 strain E1 is separated from healthy rice leaves of Yiyang Taojiang in Hunan, and is identified as Bacillus velezensis through 16S rRNA, gyrB and rpoB polygene sequencing, and is preserved in China center for type culture collection, with a preservation number of CGMCC No.35057 and a preservation date of 2025-07-03.
2. 2. Use of bacillus belgium E1 according to claim 1 for increasing resistance to rice sheath blight.
3. 3. The use according to claim 2, wherein the bacillus beijerinus E1 is used as a working fluid of a bacterial suspension.
4. 4. The application of claim 3, wherein the preparation method of the bacterial suspension working solution comprises the steps of performing inclined plane activation on bacterial strains, adjusting OD600 = 0.8, and adding Tween-20 with a volume fraction of 0.02% to obtain the bacterial suspension working solution.
5. 5. The use according to claim 3, wherein the bacterial suspension is used at a concentration of 1 x 10 8 CFU/mL.
6. 6. The use according to claim 2, characterized in that bacillus beijerinus E1 is used for the control of the four-leaf stage of rice.
7. 7. The use according to claim 2, wherein bacillus beijensis E1 is used for activating the immune pathway of rice OsMAPK-OsWRKY 13-OsOPR, inhibiting the growth of bacterial blight bacteria.

Description

Application of bacillus bailii E1 in improving rice bacterial leaf blight resistance Technical Field The invention belongs to the technical field of biological control, relates to control of bacterial leaf blight of rice, and in particular relates to application of bacillus beijerinckii E1 in improving bacterial leaf blight resistance of rice. Background Bacterial leaf Blight (Bacterial blast) is a vascular bundle Bacterial disease caused by Xanthomonas oryzae pathogenic varieties (xoos) and is recognized as one of the most damaging diseases in asia and even global rice areas. The disease mainly infects rice leaves, leads to leaf dryness and sharp reduction of photosynthesis area, can cause dead rice plants when serious, generally causes 10% -30% yield loss in years, and can even be killed in individual fields in epidemic years, thus forming continuous threat to grain safety production. For a long time, the prevention and treatment of bacterial leaf blight of rice mainly depend on chemical pesticides, such as copper preparations like bordeaux mixture and antibiotics like streptomycin. However, this strategy of chemical control-dependent is facing an increasingly serious challenge. Firstly, the Xoo pathogenic bacteria are extremely easy to generate drug resistance under continuous selection pressure, so that the drug control effect is obviously reduced. Secondly, the abuse of antibiotics and heavy metal copper can cause the problem of pesticide residue, thereby not only threatening the food safety, but also polluting the ecological environment of the rice field and damaging the micro-ecological balance and the biodiversity of the soil. In view of the limitations of chemical control, biological control (biocontrol) by utilizing beneficial microorganisms is regarded as a sustainable alternative or complementary strategy because of the advantages of environmental friendliness, difficulty in generating resistance and the like. Among many biocontrol microorganisms, bacillus (bacillus spp) is considered an ideal biocontrol bacterial resource because of its ability to produce heat-resistant spores to resist adverse environments and secrete a variety of antibacterial substances (e.g., antibacterial lipopeptides, enzymes) while having the ability to Induce Systemic Resistance (ISR) in plants. However, the existing bacillus biocontrol bacteria still have obvious short plates in actual field application, and the core problem is that the colonization capability and stability of the strain on the surface of crops are greatly limited by field complex environmental factors (such as temperature, humidity, ultraviolet rays, indigenous microbial communities and the like), so that the control effect is unstable, and the large-scale commercial popularization and application are difficult to realize. Bacillus belicus (Bacillus velezensis) has been widely reported as an important species in the genus Bacillus to have broad-spectrum antagonistic activity against various plant pathogenic bacteria such as Magnaporthe oryzae causing rice blast, rhizoctonia solani causing banded sclerotial blight. However, the research and patent resources at present are focused on the diseases, and bacillus beliensis strains which have definite prevention effect and are protected by patents and are aimed at another serious disease of rice, namely bacterial leaf blight, still belong to the blank. More importantly, there is no in-depth and clear research report on how such biocontrol bacteria act on a rice-Xoo interaction system, and the potential molecular action mechanism of the biocontrol bacteria, especially whether the biocontrol bacteria can activate the immune defense pathway of rice to resist bacterial leaf blight or not. Therefore, the bacillus belicus patent strain with stable and efficient inhibiting capability on the bacterial leaf blight of rice is separated and screened, the biocontrol mechanism of the bacillus belicus patent strain is clarified, and the bacillus belicus patent strain has important theoretical and practical significance for developing novel biopesticide, reducing the use of chemical pesticides and promoting the development of green prevention and control technology of rice diseases. The present invention is based on this urgent need. In conclusion, the invention provides the method for inhibiting the Xoo and improving the resistance of the rice leaf blight by chemotactic colonization and activation OsMAPK-OsWRKY 13-OsOPR10 immune pathway of the strain E1 (CGMCC No. 35057). Disclosure of Invention The invention aims to provide application of bacillus subtilis E1 in improving rice bacterial leaf blight resistance, and application of bacillus subtilis E1 bacterial suspension to rice bacterial leaf blight control provides a novel approach and means for rice bacterial leaf blight control. In order to achieve the above purpose, the technical scheme of the invention is as follows: The invention provides bacillus beleiensis E1, w