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CN-122012316-A - Pseudomonas brassicae with boron dissolving characteristic, screening method and application thereof

CN122012316ACN 122012316 ACN122012316 ACN 122012316ACN-122012316-A

Abstract

The invention belongs to the technical field of microorganisms and biological products thereof, and particularly relates to pseudomonas brassicae with boron dissolving property, a screening method and application thereof. The preservation number of the pseudomonas brassicae is CCTCC NO: M2026072. The pseudomonas brassicae TB-01 provided by the invention can promote plant growth by activating dual mechanisms of soil boron and secreting biomass auxin (IAA), so that the problem of growth disorder of crops in a boron-deficient soil or high-boron ore environment is solved more effectively, the dual targets of efficient utilization of boron elements in soil with insufficient effective boron supply or crops with high requirement on boron elements and healthy growth of plants are realized, and the pseudomonas brassicae has good application potential and market value.

Inventors

  • SONG BAIQUAN
  • WANG WENYA
  • ZHAO MEIHUI
  • YANG SONGLIN

Assignees

  • 黑龙江大学

Dates

Publication Date
20260512
Application Date
20260120

Claims (10)

  1. 1. A Pseudomonas rape is characterized in that the preservation number is CCTCC NO: M2026072.
  2. 2.A microbial agent comprising pseudomonas brassicae as claimed in claim 1.
  3. 3. The microbial agent according to claim 2, wherein the microbial agent is a liquid microbial agent, and the concentration of the bacterial liquid of pseudomonas brassicae in the liquid microbial agent is 0.5-1.8 in terms of OD 600 .
  4. 4. The microbial agent according to claim 2, wherein the preparation method of the microbial agent comprises the following steps: Inoculating the pseudomonas brassicae to a liquid culture medium for culture, centrifugally collecting thalli after the culture is in a logarithmic phase, washing the thalli with physiological saline, adding the physiological saline for resuspension, and regulating the concentration OD 600 value of the bacterial liquid to be 0.5-1.8 to obtain the microbial inoculum.
  5. 5. The microbial agent according to claim 4, wherein the liquid culture medium is LB culture medium or inorganic salt culture medium, wherein the inorganic salt culture medium comprises ：MgSO 4 ·7H 2 O 0.15 g/L、NaH 2 PO 4 0.08 g/L、Na 2 HPO 4 0.09 g/L、(NH 4 ) 2 SO 4 0.065 g/L、CaCl 2 0.02 g/L and glucose 2 g/L, and the pH is 6.8-7.2; the culture conditions are 28-30deg.C, 150-200rpm.
  6. 6. Use of pseudomonas brassicae according to claim 1 or of a microbial agent according to any one of claims 2-5, wherein said use is selected from at least one of the following (1) - (3): (1) The use in dissolving boron-containing minerals; (2) The application in improving the effective boron content of soil; (3) And application in promoting crop growth.
  7. 7. A method of promoting crop growth comprising the steps of: Applying the pseudomonas brassicae of claim 1 or the microbial inoculant of any one of claims 2-5 to crop root soil or foliar spray treatment, followed by culturing the crop plant.
  8. 8. The method for promoting crop growth as claimed in claim 7, wherein the pseudomonas brassicae or the microbial agent is applied to the crop root soil by a root irrigation method; The root irrigation dose is 60-100 mL per plant, and the bacterial concentration is 0.5-1.8 calculated by OD 600 .
  9. 9. The method of claim 7, wherein the crop is sugar beet and the root filling period is a seedling stage, a rapid growth stage of leaf clusters, a root sugar increasing stage and a sugar accumulating stage.
  10. 10. A screening method of boron-dissolving bacteria is characterized in that, pseudomonas brassicae for screening according to claim 1, comprising the steps of: s1, culturing a collected soil sample in an LB culture medium, and separating and purifying soil microorganisms; s2, culturing the purified soil microorganism in an inorganic salt culture medium containing boron ore; S3, collecting culture solution, filtering, detecting the content of soluble boron in the filtrate, and screening microorganisms with boron dissolving characteristics according to the content of the soluble boron.

Description

Pseudomonas brassicae with boron dissolving characteristic, screening method and application thereof Technical Field The invention relates to the technical field of microorganisms and biological products thereof, in particular to pseudomonas brassicae with boron dissolving property, a screening method and application thereof. Background Boron is a trace nutrient essential for plants and plays a very important role in plant cell wall synthesis, carbohydrate transport, development of reproductive organs, photosynthesis of plants, and the like. The phenomenon of boron deficiency of soil is very common in the global scope, and the yield and quality of various crops such as cotton, rape, beet, citrus and the like and fruits are seriously affected. In agricultural production, the problem of boron deficiency is mainly corrected by a method of applying chemical boron fertilizer such as borax and the like. However, boron behaves very specifically in the soil and its effectiveness depends strongly on the pH of the soil, in acidic soil boron is easily immobilized by iron aluminum oxide, while in alkaline or lime soil boron effectiveness drops drastically, resulting in very low, usually less than 5%, utilization of the applied boron fertilizer in the season. This traditional way of applying fertilizers not only increases the production costs, but also presents environmental risks due to leaching or excessive application. In the field of plant nutrition, the use of beneficial microorganisms, such as plant rhizosphere growth promoting bacteria (PGPR), to activate mineral elements inherent in soil that are difficult to directly use by plants has proven to be a green and sustainable agricultural strategy. The phosphate-dissolving microorganism can release the phosphorus element in the indissoluble phosphate in the soil through the excellent phosphate-dissolving capability, so that the content of the soluble phosphorus in the soil is improved, the utilization rate of the phosphorus element in the soil is improved, and the related microbial inoculum is widely applied to agricultural production. However, no report has been made on microorganisms having boron-dissolving and boron-dissolving properties, and no study has been made on improving the utilization rate of effective boron in soil by using microorganisms. Therefore, the screening of microorganisms capable of dissolving indissolvable boron or the development of biological products thereof is an important research direction for reducing boron fertilizer use and boron resource consumption of boron-deficient soil, improving the utilization efficiency of soil boron and promoting green sustainable development of agricultural production. Disclosure of Invention Aiming at the defect of the prior art that microorganisms with boron dissolving and dissolving characteristics are lack, the invention provides pseudomonas brassicae TB-01 with boron dissolving capability. Based on the excellent characteristics that the pseudomonas rape strain can promote plant growth by activating soil boron and secreting biomass-promoting auxin, the application of the pseudomonas rape strain or a microbial agent containing the pseudomonas rape strain in dissolving boron-containing minerals, improving the effective boron content of soil and/or promoting crop growth is further provided. The invention is realized by the following technical scheme: the first aspect of the invention provides pseudomonas brassicae with the preservation number of CCTCC NO: M2026072. In a second aspect the invention provides a microbial agent comprising Pseudomonas brassicae as described above. Further, the microbial agent is a liquid microbial agent, and in the liquid microbial agent, the concentration of the bacterial liquid of the pseudomonas brassicae is 0.5-1.8 calculated by OD 600. Further, the preparation method of the microbial agent comprises the steps of inoculating the pseudomonas brassicae in a liquid culture medium for culture, centrifugally collecting thalli after the culture is carried out to a logarithmic phase, washing the thalli with physiological saline, adding the physiological saline for resuspension, and adjusting the concentration OD 600 value of the bacterial liquid to be 0.5-1.8 to obtain the microbial agent. Further, the liquid culture medium is LB culture medium or inorganic salt culture medium, wherein the inorganic salt culture medium comprises ：MgSO4·7H2O 0.15 g/L、NaH2PO4 0.08 g/L、Na2HPO4 0.09 g/L、(NH4)2SO4 0.065 g/L、CaCl20.02 g/L and glucose 2 g/L, and the pH value is 6.8-7.2. Further, the culture conditions are 28-30℃and 150-200rpm. In a third aspect, the present invention provides the use of a Pseudomonas brassicae or a microbial agent as described above, selected from at least one of the following (1) - (3): (1) The use in dissolving boron-containing minerals; (2) The application in improving the effective boron content of soil; (3) And application in promoting crop growth. In a fourth aspec