CN-122012318-A - Bacillus subtilis KY2098, compound preparation of bacillus subtilis KY2098 and grape seed extract and application of compound preparation in preventing and treating chicken bursal mycoplasmosis

Abstract

The invention belongs to the technical field of biological preparations, and discloses bacillus subtilis KY2098, a compound preparation of the bacillus subtilis KY2098 and grape seed extract and application of the bacillus subtilis KY2098 and the compound preparation in preventing and treating chicken bursa mycoplasmosis. The preservation number of the bacillus subtilis is CCTCC NO: M2026185. Experiments prove that the bacillus subtilis and grape seed extract compound preparation has potential interaction, can be matched with each other, plays a role in synergy, can effectively prevent and treat chicken bursal mycoplasmosis, has the effect of enhancing organism immunity and promoting growth, and has the advantages of scientific, efficient, green and environment-friendly treatment mode, and has important significance for the industrial development of non-resistance cultivation.

Inventors

• KANG CHAO
• SUN XIAOMEI
• GAO JINGE
• LIU YANG
• JIN MEILIN
• YAN JIAHAO
• JIANG LINGYU
• CUI HONGYAN
• LI QIAN
• DENG MINGYONG

Assignees

• 华中农业大学

Dates

Publication Date

20260512

Application Date

20260125

Claims (9)

1. 1. An isolated bacillus subtilis (Bacillus subtilis) KY2098 with the preservation number of CCTCC NO: M2026185.
2. 2. The fermentation broth or supernatant of bacillus subtilis according to claim 1, wherein the fermentation broth comprises live bacillus subtilis KY 2098.
3. 3. A compound formulation comprising bacillus subtilis KY2098 of claim 1, a fermentation broth or a fermentation supernatant thereof.
4. 4. The compound preparation according to claim 3, wherein the compound preparation contains grape seed extract.
5. 5. The compound preparation of claim 4, wherein the grape seed extract is an aqueous grape seed extract.
6. 6. The method for preparing a compound preparation according to claim 5, wherein the method comprises extracting pulverized and sieved grape seeds with water at a ratio of 1:5-20 for several times, mixing filtrates, heating and refluxing, ultrafiltering, concentrating, and spray drying.
7. 7. The compound preparation of claim 4, wherein the mass ratio of the bacillus subtilis KY2098 powder to the grape seed extract is 1:0.005-0.02, and the effective bacterial concentration of the bacillus subtilis KY2098 powder is 5.0X10 9 CFU/g.
8. 8. The bacillus subtilis KY2098 of claim 1, the fermentation broth or supernatant of the bacillus subtilis KY2098 of claim 2, or any of the following applications of the compound formulation of claim 3: 1) The application of the composition in preparing medicines for treating or preventing chicken bursal mycoplasmosis; 2) The application of the composition in preparing medicines for treating or preventing chicken bursa mycoplasma infection; 3) The application in preparing chicken feed additive.
9. 9. The use according to claim 8, wherein the pharmaceutical dosage form is all pharmaceutically acceptable dosage forms.

Description

Bacillus subtilis KY2098, compound preparation of bacillus subtilis KY2098 and grape seed extract and application of compound preparation in preventing and treating chicken bursal mycoplasmosis Technical Field The invention belongs to the technical field of biological preparations, and particularly relates to bacillus subtilis KY2098, a compound preparation of the bacillus subtilis KY2098 and grape seed extract and application of the bacillus subtilis KY2098 and the compound preparation in preventing and treating chicken bursal mycoplasmosis. Background Chicken bursal mycoplasmosis is a global infectious disease caused by bursal mycoplasmosis, and causes serious economic loss to poultry industry. The main symptoms of the disease are soft feet, emaciation and keel cyst, which can have adverse effects on the egg laying performance, egg quality and growth of the breeding hens and laying hens. Improper feeding management, immunosuppression, and mixed infection with other diseases can all contribute to the disease's prevalence. With the rapid development of intensive breeding, the incidence of chicken bursal mycoplasmosis is in an ascending trend, and the chicken bursal mycoplasmosis is a serious threat to the poultry industry. At present, medicines for treating chicken bursal mycoplasmosis are more in the market, but due to different culture environments and management levels, and individual and regional differences exist in the sensitivity of chicken bursal mycoplasmosis to medicines, a unified and standardized treatment scheme for chicken bursal mycoplasmosis is lacking in clinic by veterinarians. The common treatment of chicken bursal mycoplasmosis depends on the addition of antibiotics. With the prevalence and development of chicken bursal mycoplasmosis, pathogen resistance is continuously enhanced, and the problem of drug residue is also more serious, so that the existing treatment scheme is more and more limited, and a more scientific and effective treatment strategy needs to be explored. With the advent of the "anti-reduction/anti-replacement age", the development and application of antibiotic substitutes became a hotspot problem for research and production in animal husbandry, where the development of probiotics, plant extracts and traditional Chinese medicine products has received a great deal of attention. As a feed additive, the bacillus subtilis has the potential of improving intestinal microbiota of livestock and poultry, regulating metabolism, enhancing immunity and promoting digestion and absorption of feed. Grape seed Extract (GRAPE SEED Extract, GSE) is an active ingredient extracted from grape seeds, mainly contains oxidation-resistant substances such as procyanidins (OPCs), polyphenol compounds and the like, can improve the quality and the production performance of livestock and poultry meat, enhance the oxidation resistance and the immunity of animals, and has application potential for replacing antibiotics. The application combines probiotics and plant extracts to prepare the bacillus subtilis-grape seed extract composite product, and the bacillus subtilis-grape seed extract composite product have a synergistic effect, provide a new strategy for the treatment of chicken bursa mycoplasma diseases, and accord with the scientific concepts of safety, environmental protection and green cultivation. Disclosure of Invention Aiming at the problems in the prior art, the invention aims to provide the bacillus subtilis KY2098 for preventing and treating chicken bursal mycoplasmosis, and the preservation number of the strain is CCTCC NO: M2026185. Another object of the present invention is to provide a strain and its use in the preparation of a compound formulation with grape seed extract. In order to solve the technical problems, the invention provides the following technical scheme: The applicant has screened a strain of bacillus subtilis with antagonistic chicken bursa mycoplasma from a plurality of strains of bacillus subtilis, and the strain is preserved in 2026, 01 and 22 days to China center for type culture Collection, and is classified and named as bacillus subtilis (Bacillus subtilis) KY2098, with the preservation number of CCTCC NO: M2026185 and the address of university of Wuhan in China. The protection scope of the invention comprises: a fermentation broth or fermentation supernatant of bacillus subtilis (Bacillus subtilis) KY2098, the fermentation broth comprising viable bacillus subtilis KY 2098. A compound preparation comprising bacillus subtilis KY2098, a fermentation broth or a fermentation supernatant thereof. The compound preparation described above, preferably, the compound preparation also contains grape seed extract. Preferably, the grape seed extract is a grape seed water extract. The preparation method of the compound preparation, preferably, the grape seed extract comprises the steps of extracting crushed and sieved grape seeds for a plurality of times according to the materia