CN-122012319-A - Lactobacillus reuteri, mycorrhizal fungi synthetic flora containing Luo He bacillus and arbuscular mycorrhizal fungi, compound microbial inoculum and application of compound microbial inoculum in soil nitrogen fixation and plant growth promotion

Abstract

The invention belongs to the technical field of biological bactericides and crop cultivation, and particularly relates to a bacillus, a synthetic flora containing Luo He bacillus and arbuscular mycorrhizal fungi, a composite bactericides and application of the composite bactericides in soil nitrogen fixation and plant growth promotion. The bacillus subtilis SBE2523 is obtained through separation and is subjected to biological preservation, and the Luo He bacillus has the effects of promoting plant growth and improving the soil nitrogen fixation capacity. On the basis, the invention provides a synthetic flora containing Luo He bacillus SBE2523 and arbuscular mycorrhizal fungi and a composite microbial agent containing the synthetic flora, achieves the effect of single fungus better than Luo He bacillus SBE2523 through the synergistic symbiotic effect of two microorganisms, has more remarkable effect of promoting plant growth and improving the soil nitrogen fixation capacity, and overcomes the problems of low infection efficiency, unstable growth promotion effect, poor environmental adaptability and the like of the existing single microbial agent in practical application, thereby realizing the multiple aims of improving crop yield and soil nitrogen fixation.

Inventors

• CHU HAIYAN
• LV LIHUI
• CHEN SIYU
• QIAO MENGJIE
• ZHANG YUXIAO
• NIE LI

Assignees

• 中国科学院南京土壤研究所

Dates

Publication Date

20260512

Application Date

20260126

Claims (10)

1. 1. A strain of Lactobacillus reuteri (Rhodanobacter sp.) SBE2523, wherein the preservation number of the Luo He bacillus SBE2523 is CGMCC No.36390.
2. 2. A microbial preparation comprising the Bacillus Luo He SBE2523 as claimed in claim 1.
3. 3. The microbial agent of claim 2, wherein the microbial agent comprises a fermentation broth of Luo He bacillus SBE2523 or a bacterial suspension of lactobacillus reuteri SBE 2523.
4. 4. The microbial inoculum according to claim 2 or 3, wherein the effective viable bacteria concentration of the lactobacillus reuteri SBE2523 in the microbial inoculum is equal to or greater than 1 x 10 9 CFU/mL.
5. 5. A synthetic flora comprising arbuscular mycorrhizal fungi (Rhizophagus irregularis) and the Luo He bacillus SBE2523 according to claim 1.
6. 6. The synthetic population of claim 5, wherein the arbuscular mycorrhizal fungi comprises rhizopus morphous.
7. 7. A composite microbial inoculant comprising the synthetic flora of claim 5 or 6.
8. 8. The composite microbial inoculant according to claim 7, wherein the composite microbial inoculant comprises arbuscular mycorrhizal fungus inoculant and Luo He bacillus inoculant, the mass ratio of the arbuscular mycorrhizal fungus inoculant to the Luo He bacillus inoculant is 10 g to 1 mL, the arbuscular mycorrhizal fungus inoculant comprises spores, hyphae, infected root systems and culture matrixes, the content of the spores in the arbuscular mycorrhizal fungus inoculant is 50-70/g, and the effective viable bacteria concentration of the lactobacillus reuteri SBE2523 in the Luo He bacillus inoculant is more than or equal to 1X 10 9 CFU/mL.
9. 9. Use of Luo He bacillus SBE2523 according to claim 1 or a microbial agent according to any one of claims 2 to 4 or a synthetic flora according to claim 5 or 6 or a composite microbial agent according to claim 7 or 8 for increasing soil nitrogen fixation and/or for promoting plant growth.
10. 10. A method for improving the nitrogen fixation capacity of soil and/or promoting the growth of plants is characterized by comprising the step of inoculating the microbial inoculum of any one of claims 2-4 or the composite microbial inoculum of claim 7 or 8 into cultivation soil or cultivation matrix.

Description

Lactobacillus reuteri, mycorrhizal fungi synthetic flora containing Luo He bacillus and arbuscular mycorrhizal fungi, compound microbial inoculum and application of compound microbial inoculum in soil nitrogen fixation and plant growth promotion Technical Field The invention belongs to the technical field of biological bactericides and crop cultivation, and particularly relates to a bacillus, a synthetic flora containing Luo He bacillus and arbuscular mycorrhizal fungi, a composite bactericides and application of the composite bactericides in soil nitrogen fixation and plant growth promotion. Background Nitrogen is a core nutrient element essential for the growth and development of crops. In intensive agricultural production, the high demand of crops for nitrogen is mainly met by the investment of chemical nitrogenous fertilizers, which is accompanied by huge energy consumption and production cost, and a series of serious environmental problems such as soil acidification, water eutrophication, greenhouse gas emission and the like are caused. Although certain soil microorganisms are capable of biological nitrogen fixation, this process is often accompanied by strong uptake of nitrogen by plants and continued crop harvest, resulting in the ubiquitous absence of nitrogen from the soil. Meanwhile, the modern cultivation mode reduces nutrient return, further aggravates soil nitrogen unbalance and interferes with nitrogen circulation in the nature. The lack and excess of nitrogen can have important influence on the health and production capacity of the global ecological system, and although human beings invest far more resources than other elements in nitrogen management and regulation, how to effectively integrate biological nitrogen fixation, optimize soil nitrogen circulation and maintain nitrogen dynamic balance still remains a core scientific challenge and practice bottleneck for realizing sustainable transformation of agriculture. However, nitrogen fixation resources in natural soil and their efficacy are far from being effectively utilized. The traditional strategy for strengthening biological nitrogen fixation at present faces obvious limitations that firstly, the operation is simple and convenient by using a single microbial inoculum for inoculation, but partial exogenous strains often cause functional failure and effect fluctuation due to insufficient competitiveness and unstable rhizosphere colonization in complex field environments, secondly, the method can improve the soil environment by optimizing agricultural measures (such as leguminous rotation or increasing application of organic fertilizers), but has slow nitrogen fixation effect, is limited by regional climate, soil and planting system and has insufficient universality, and thirdly, the breeding of high-efficiency symbiotic crops is fundamentally limited by deficient germplasm resources, long breeding period and the inhibition of nitrogen fixation efficiency by field water and fertilizer management. The common shortboard of these traditional approaches is that none of them fundamentally solve the core dilemma of "difficult to survive, poorly colonize, and poorly functioning" functional microorganisms in real farmlands. Therefore, agricultural practices are in need of a new biological nitrogen fixation technology capable of actively adapting to complex environments and stably functioning. In recent years, the cross fusion of the synthetic biology and the microbial ecology provides a new idea for solving the problems. The synthetic microbial community is constructed into a microbial team with stable structure and high efficiency by rationally designing and combining a plurality of specific strains with complementary functions and mutually beneficial symbiosis. However, there is a lack of single and synthetic flora in the art that combines efficient nitrogen fixation with multiple growth promoting functions. Disclosure of Invention The invention aims to provide a bacillus subtilis, a synthetic flora containing Luo He bacillus and arbuscular mycorrhizal fungi, a composite microbial inoculum and application thereof in soil nitrogen fixation and plant growth promotion, wherein the Luo He bacillus SBE2523, the synthetic flora containing Luo He bacillus SBE2523, the microbial inoculum containing Luo He bacillus SBE2523 or the composite microbial inoculum containing the synthetic flora have the effects of promoting plant growth and improving soil nitrogen fixation. The invention provides a strain of bacillus rotundiensis SBE2523, wherein the preservation number of the bacillus Luo He SBE2523 is CGMCC No.36390. The invention also provides a microbial inoculum, which comprises Luo He bacillus SBE2523 according to the technical scheme. Preferably, the microbial inoculum comprises a fermentation broth of Luo He bacillus SBE2523 or a bacterial suspension of lactobacillus reuteri SBE 2523. Preferably, the effective viable bacteria concentration of the lactob