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CN-122012322-A - Microbubble bacteria and application of microbial inoculum thereof in saline-alkali soil

CN122012322ACN 122012322 ACN122012322 ACN 122012322ACN-122012322-A

Abstract

The invention relates to the field of agricultural microorganism technology and soil remediation, in particular to application of microbubble bacteria and microbial inoculum thereof in saline-alkali soil, wherein the strain is named microbuldifer sp.PM1, the preservation unit is China center for type culture Collection, the preservation acceptance date is 2026, 1 month and 4 days, and the preservation number is CGMCC NO:37273. Through experiments and determination, the microbial agent prepared by using PM1 has important application value in the field of saline-alkali soil improvement, especially in high-quality yield-increasing cultivation of corn.

Inventors

  • SHEN ZHONGHUA
  • HE DONGLIN
  • LI CHENGGANG
  • WANG LIYAN
  • LV XIAO
  • YUAN XIAONING
  • CHEN KANG
  • LU ZHENLEI

Assignees

  • 山东正元环境科技有限公司

Dates

Publication Date
20260512
Application Date
20260204

Claims (10)

  1. 1. Microbubble bacteria for promoting corn growth, and its strain name is Microbulbifer sp.PM1, and the preservation unit is China center for type culture Collection, the preservation acceptance date is 2026, 1 month and 4 days, and the preservation number is CGMCC NO. 37273.
  2. 2. The microbubble bacterium according to claim 1, wherein the microbubble bacterium is used for improving saline-alkali soil.
  3. 3. The microbial agent is characterized by comprising the microbubble bacterial strain PM1 as claimed in any one of claims 1-2 and a nutritional aid, wherein the nutritional aid preferably comprises at least one of humic acid, compound amino acid and trace elements, and the trace elements preferably comprise at least one of zinc sulfate, boric acid and sodium molybdate.
  4. 4. The microbial agent according to claim 3, which is characterized by comprising a microbubble bacterial strain PM1, humic acid, compound amino acid and trace elements, wherein the content of the humic acid is 10% -20% of the total mass of the composition, the content of the compound amino acid is 5% -10%, the content of the trace elements is 0.5% -1%, and more preferably, the content of the humic acid is 15% of the total mass of the composition, the content of the compound amino acid is 7.5% and the content of the trace elements is 0.75% in terms of weight ratio.
  5. 5. The microbial agent according to any one of claims 3 to 4, wherein the microbubble bacterial strain PM1 is obtained by fermentation, and the effective viable count in the bacterial liquid is not less than 1X 10 8 CFU/mL.
  6. 6. The microbial agent according to claim 5, wherein the preparation process of the microbial agent comprises the following steps: s1, preparation of seed liquid Inoculating a microbubble strain PM1 into an LB liquid culture medium, and performing shake culture to obtain a seed solution A containing the strain PM 1; S2, preparation of microbial liquid Diluting the seed liquid A, adding the diluted seed liquid A into fermentation broth at a ratio of 0.5-1.5%, and performing shaking culture to obtain seed liquid B containing bacterial strain PM 1; s3, preparation of microbial agent Placing 1L of seed liquid B into a container, adding humic acid, amino acid and microelements, and uniformly mixing to obtain microbial agent.
  7. 7. The microbial agent according to claim 5, wherein the preparation process of the microbial agent comprises the following steps: s1, preparation of seed liquid Inoculating the microbubble strain PM1 into an LB liquid culture medium, performing shake culture at 30 ℃ and 180 rpm for 48 hours to obtain seed liquid A with the effective viable count of more than or equal to 1X 10 8 CFU/mL, and diluting to 1X 10 8 ~ 1×10 10 CFU/mL when in use; S2, preparation of microbial liquid Inoculating the seed liquid A into the fermentation liquid according to the proportion of 1%, and carrying out shaking culture for 48 hours at 30 ℃ and 180 rpm to obtain a bacterial liquid B with the effective viable count of more than or equal to 1 multiplied by 10 8 CFU/mL; s3, preparation of microbial agent And (3) taking bacterial liquid 1L in the step (S2), and uniformly mixing with 50-100 g of humic acid, 20-60 g of amino acid and 2-6 g of trace elements to prepare the microbial agent.
  8. 8. The microbial agent is characterized by comprising 1 part of the bacterial liquid, 50-100 parts of humic acid, 20-60 parts of compound amino acid and 2-6 parts of trace elements according to g/L, wherein the microbial agent is prepared from 1 part of the bacterial liquid, 75 parts of humic acid, 40 parts of compound amino acid and 4 parts of trace elements according to g/L, and further preferably comprises 1 part of the bacterial liquid, 75 parts of compound amino acid and 40 parts of trace elements according to g/L, wherein the effective viable count of the microbubble bacterial strain PM1 is 1X 10 8 ~ 1×10 10 CFU/mL.
  9. 9. The microbial agent of claim 8, wherein the microbial agent is used for improving saline-alkali soil and promoting corn growth.
  10. 10. The microbial agent according to claim 8, wherein the microbial agent is applied to improving saline-alkali soil and promoting corn growth for 2-5 times in a corn growth period, and the application amount of the microbial agent is 1-5L per mu.

Description

Microbubble bacteria and application of microbial inoculum thereof in saline-alkali soil Technical Field The invention relates to the field of agricultural microorganism technology and soil remediation, in particular to a microbubble (Microbulbifer sp.) strain PM1 with efficient saline-alkali tolerance and growth promotion functions and application thereof in improving saline-alkali soil and promoting corn growth. Background Saline-alkali soil is one of the main obstacle factors limiting global agricultural production. The high salt concentration causes the osmotic pressure of soil to rise, so that the plant root system is difficult to absorb water, and meanwhile, ion poisoning and nutrition unbalance are caused, and the growth of crops is seriously inhibited. Corn is taken as a main grain crop in China, and the yield of the corn on the saline-alkali soil is obviously affected. Currently, methods for improving saline-alkali soil mainly include physical improvement (such as salt irrigation), chemical improvement (such as gypsum application and phosphogypsum application) and biological improvement. Physical and chemical methods are costly and may cause secondary pollution or have a non-persistent effect. Biological improvement, particularly the utilization of saline-alkali tolerant plant growth promoting bacteria, is an environmentally friendly and sustainable strategy. The plant growth promoting bacteria promote plant growth and strengthen stress resistance through nitrogen fixation, phosphorus dissolution, secretion of plant hormone and other mechanisms. However, plant growth promoting bacteria reported in the prior art tend to have poor colonization capability and unstable effect in complex saline-alkali soil environments. Therefore, the method is used for separating and screening excellent strains which are high-efficiently suitable for saline-alkali stress, and developing an application method thereof, and has important significance for utilizing saline-alkali soil and guaranteeing grain safety. Disclosure of Invention The invention aims to overcome the defects of the prior art and provide a burkholderia strain which has extremely strong saline-alkali tolerance. The strain is named Microbulbifer sp.PM1, the preservation unit is China center for type culture Collection, the preservation acceptance date is 2026, 1 month and 4 days, and the preservation number is CGMCC NO. 37273. Furthermore, the application of the microbubble bacteria in improving the saline-alkali soil, in particular the application in improving and promoting the corn growth in the saline-alkali soil has obvious effects. Further, the invention provides a microbial agent, wherein the microbial agent comprises a microbubble bacterial strain PM1 and a nutrition auxiliary agent, preferably, the nutrition auxiliary agent comprises at least one of humic acid, compound amino acid and trace elements, and preferably, the trace elements comprise at least one of zinc sulfate, boric acid and sodium molybdate. Further, the microbial agent comprises a microbubble bacterial strain PM1, humic acid, compound amino acid and trace elements, wherein the content of the humic acid is 10% -20% of the total mass of the composition, the content of the compound amino acid is 5% -10%, the content of the trace elements is 0.5% -1%, and more preferably, the content of the humic acid is 15% of the total mass of the composition, the content of the compound amino acid is 7.5%, and the content of the trace elements is 0.75% in terms of weight ratio. Further, the microbubble bacterial strain PM1 is obtained through fermentation, and the effective viable count in the bacterial liquid after fermentation is more than or equal to 1 multiplied by 10 8 CFU/mL. Further, the preparation process of the microbial agent comprises the following steps: s1, preparation of seed liquid Inoculating a microbubble strain PM1 into an LB liquid culture medium, and performing shake culture to obtain a seed solution A containing the strain PM 1; S2, preparation of microbial liquid Diluting the seed liquid A, adding the diluted seed liquid A into fermentation broth at a ratio of 0.5-1.5%, and performing shaking culture to obtain seed liquid B containing bacterial strain PM 1; s3, preparation of microbial agent Placing 1L of seed liquid B into a container, adding humic acid, amino acid and microelements, and uniformly mixing to obtain microbial agent. Further, the preparation process of the microbial agent comprises the following steps: s1, preparation of seed liquid Inoculating the microbubble strain PM1 into an LB liquid culture medium, performing shake culture at 30 ℃ and 180 rpm for 48 hours to obtain seed liquid A with the effective viable count of more than or equal to 1X 10 8 CFU/mL, and diluting to 1X 10 8 ~ 1×1010 CFU/mL when in use; S2, preparation of microbial liquid Inoculating the seed liquid A into the fermentation liquid according to the proportion of 1%, and carrying out shaking c