CN-122012323-A - Serratia strain ZNL1 and application thereof

Abstract

The invention belongs to the technical field of agricultural microorganisms, and discloses a Serratia strain ZNL and a microbial agent, a preparation method and application thereof. The strain ZNL is preserved in China general microbiological culture Collection center (CGMCC) No.37364, can efficiently decompose organic nitrogen, inorganic phosphorus, potassium and zinc and secrete siderophores, has obvious drought resistance, and can promote plant growth by means of generating plant hormone, promoting nutrient element absorption and the like. The invention also provides a liquid or solid microbial inoculum containing the strain and a preparation method thereof, and the microbial inoculum can be used for activating soil nutrients, promoting plant growth and improving drought resistance, is especially suitable for cultivation of crops such as fruit trees and the like, and has wide agricultural application prospect.

Inventors

• LIU LI
• TAO XU
• ZENG XIANGHONG
• GUO SHUWEI

Assignees

• 镇宁布依族苗族自治县农业农村局

Dates

Publication Date

20260512

Application Date

20260206

Claims (8)

1. 1. A serratia strain ZNL1, wherein the strain ZNL is deposited in the China general microbiological culture collection center (CGMCC) with a accession number of 37364, which is 1 month 12 of 2026.
2. 2. A microbial inoculant of serratia strain ZNL1, comprising serratia strain ZNL according to claim 1.
3. 3. The microbial agent of claim 2, wherein the agent is a liquid agent or a solid agent comprising the strain or a fermentation product thereof.
4. 4. A microbial agent according to claim 3, wherein the solid microbial agent is obtained by mixing and drying a fermentation broth comprising the strain ZNL1 with a carrier selected from turf, vermiculite, diatomaceous earth or a combination thereof.
5. 5. A method for preparing a microbial agent of Serratia strain ZNL1, which is characterized by comprising the following steps: (1) Inoculating Serratia strain ZNL1 according to claim 1 into a liquid culture medium for fermentation culture; (2) When the number of viable bacteria in the fermentation broth reaches more than 1×10 9 CFU/mL, the fermentation is terminated, and a fermentation broth containing the strain ZNL1 is obtained.
6. 6. The method according to claim 5, further comprising the step of mixing the fermentation broth obtained in the step (2) with a carrier and drying the mixture to obtain a solid microbial inoculum.
7. 7. Use of the serratia strain ZNL1 according to claim 1 for the preparation of a microbial preparation for activating soil nutrients.
8. 8. The use of claim 7, wherein the activated soil nutrient comprises at least one of organic nitrogen-solubilizing, inorganic phosphorus-solubilizing, potassium-solubilizing, zinc-solubilizing, and siderophores-secreting.

Description

Serratia strain ZNL1 and application thereof Technical Field The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a Serratia strain ZNL and application thereof. Background With the development of agricultural modernization, the problems of soil fertility decline, nutrient imbalance, ecological environment destruction and the like caused by unreasonable fertilizer application are increasingly prominent. The microbial preparation is used as an environment-friendly agricultural input product, can activate nutrients which are difficult to utilize in soil through metabolic activity of bacterial strains, promote plant nutrient absorption, strengthen plant stress resistance, and plays an important role in improving soil and improving crop yield and quality. However, the existing microbial preparation still has remarkable limitations that on one hand, partial strains of Serratia are proved to have the functions of biological nitrogen fixation, phosphorus dissolution, phytohormone secretion and the like, but related researches are mostly focused on single functions, and lack of systematic exploration on the multi-functional integration of the strains, so that the functions are single and the pertinence is not strong in practical application, and on the other hand, the efficacy of the composite microbial preparation is often reduced due to incompatibility or antagonism among the strains, and the general microbial preparation has poor adaptability to the rhizosphere microenvironment of specific crops (if trees) and low colonization efficiency, so that the effect is difficult to be stably exerted under the stress environments such as drought and the like. Therefore, screening and separating the indigenous Serratia strain which has multiple functions and high activity and is derived from a specific habitat, developing a synergistic microbial preparation and having important significance for promoting green agricultural development. Disclosure of Invention The invention aims to provide a multifunctional Serratia strain ZNL1, a microbial agent, a preparation method and application thereof, so as to solve the problems of single function, weak stress resistance and the like of the existing microbial agent and realize the synergistic effect of soil nutrient activation, plant growth promotion and stress resistance improvement. In order to achieve the technical purpose and achieve the technical effect, the invention provides the following technical scheme: serratia strain ZNL1, wherein the strain ZNL is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.37364 in the 1 st month 12 of 2026. On the other hand, the invention also provides a microbial agent of the Serratia strain ZNL1, wherein the microbial agent comprises the Serratia strain ZNL. Further, the microbial inoculum is a liquid microbial inoculum or a solid microbial inoculum comprising the strain or a fermentation product thereof. Further, the solid microbial inoculum is prepared by mixing and drying a fermentation broth containing the strain ZNL1 with a carrier, wherein the carrier is selected from turf, vermiculite, diatomite or a combination thereof. On the other hand, the invention also provides a preparation method of the Serratia strain ZNL < 1 > microbial agent, which comprises the following steps: (1) Inoculating the Serratia strain ZNL1 into a liquid culture medium for fermentation culture; (2) When the number of viable bacteria in the fermentation broth reaches more than 1×10 9 CFU/mL, the fermentation is terminated, and a fermentation broth containing the strain ZNL1 is obtained. Further, the method also comprises the steps of mixing the fermentation liquor obtained in the step (2) with a carrier and drying to obtain the solid microbial inoculum. On the other hand, the invention also provides an application of the Serratia strain ZNL1 in preparing a microbial preparation for activating soil nutrients. Further, the activated soil nutrient comprises at least one of organic nitrogen, inorganic phosphorus, potassium, zinc and siderophores. Compared with the prior art, the invention has the beneficial effects that: 1. The strain ZNL has comprehensive functions, has multiple effects of activating soil nutrients and promoting plant stress-tolerant growth, and solves the problem of application limitation of single-function strain. 2. The zinc dissolving capability is outstanding and is 22 times of that of a blank control, the iron carrier secretion capability, the potassium dissolving capability and the drought resistance are obviously superior to those of most control strains, the organic nitrogen dissolving halo index is more than or equal to 2.0, and the application effect is obvious. 3. The microbial inoculum has simple preparation process, low cost, and convenient use, and the two forms of liquid and solid are suitable for different agricultur