CN-122012324-A - Lactobacillus plantarum for high yield of feruloyl esterase and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and provides lactobacillus plantarum with high feruloyl esterase yield and application thereof. The strain is lactobacillus plantarum Fs6 (Lactiplantibacillus plantarum), the preservation number is CGMCC No.36264, and the preservation date is 2025, 10, 20 days. The lactobacillus plantarum provided by the invention can obviously improve the fermentation performance of dough, and has good synergistic effect when being fermented together with yeast and good synergistic effect when being fermented together with yeast. The survival rate is better at the concentration of bile salt of 0.3 and 0.6g/L, which indicates that the lactobacillus Fs6 has a certain probiotic function. Fs6 has low self-aggregation force (32.77%) and hydrophobicity (32.43%) and poor adhesion. Can effectively release the combined phenolic acid in the grains, so that the nutritional value of the grains can play a better health role in human bodies.

Inventors

• ZHANG GUOHUA
• LIANG JINGJING

Assignees

• 山西大学

Dates

Publication Date

20260512

Application Date

20260209

Claims (2)

1. 1. The lactobacillus plantarum strain is characterized in that the specific name of the strain is lactobacillus plantarum Fs6, the preservation number is CGMCC No.36264, the preservation date is 2025, 10 months and 20 days, the Latin name is Lactiplantibacillus plantarum, and the strain is preserved in the China general microbiological culture Collection center of the China Committee for culture Collection of microorganisms, and the preservation unit is North Star Xili No. 1, 3 of the Korean region of Beijing city.
2. 2. Use of lactobacillus plantarum according to claim 1 for the preparation of a starter for fermenting rye.

Description

Lactobacillus plantarum for high yield of feruloyl esterase and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus plantarum for high-yield feruloyl esterase and application thereof. Background In rye, phenolic acids are mainly present in the bound state, with free phenolic acids only accounting for 1% -5% of the total. Most of the bound phenolic acids are bound to the cell wall component via ester bonds, and are partially linked to lignin structure. Feruloyl esterase (Ferulic ACID ESTERASE, FAE) can effectively break ester bonds and release combined ferulic acid and other phenolic acids, so that the antioxidant and health functions of the rye product are remarkably improved. In addition, the released phenolic acid can also perform non-covalent action or crosslinking with starch molecules to change the gelatinization, retrogradation and digestion properties of the starch, thereby helping to improve the texture stability and nutritional properties of the rye acid dough product. Therefore, screening of dominant strains with stronger FAE productivity and adaptation to fermentation environment is not only a key way for releasing rye bound phenolic acid, but also an important basis for developing high-quality functional rye acid dough and products thereof. Disclosure of Invention The invention provides a lactobacillus plantarum strain for high yield of feruloyl esterase and application thereof. The invention is realized by the following technical scheme that the high-yield feruloyl esterase lactobacillus plantarum is specifically named as lactobacillus plantarum Fs6, the preservation number is CGMCC No.36264, the preservation date is 2025, 10 months and 20 days, the Latin name is Lactiplantibacillus plantarum, and the strain is preserved in China general microbiological culture Collection center, and the preservation unit is North Star Xiyu No. 1, 3 in the Korean area of Beijing. The invention also provides application of the lactobacillus plantarum for producing feruloyl esterase in preparation of a starter for fermenting rye. The identification proves that the lactobacillus plantarum Fs6 can remarkably improve the fermentation performance of dough, shows good synergistic effect when being fermented together with yeast, and generates feruloyl esterase with the enzyme activity of 13.51+/-0.54 mU/mL. The phase is in a delay period when the time is 0-4h, the phase is in a logarithmic period when the time is 4-14h, and the stationary phase is reached after 14 h. The fermentation is combined with the yeast Ah4, so that the fermentation performance of the dough is obviously improved, and the dough has good synergistic effect when being co-fermented with the yeast. The survival rate is better at the concentration of bile salt of 0.3 and 0.6g/L, which indicates that the lactobacillus Fs6 has a certain probiotic function. Fs6 has low self-aggregation force (32.77%) and hydrophobicity (32.43%) and poor adhesion. Can effectively release the combined phenolic acid in the grains, so that the nutritional value of the grains can play a better health role in human bodies. The specific name of the strain is lactobacillus plantarum Fs6, the preservation number is CGMCC No.36264, the preservation date is 2025, 10 and 20 days, the Latin name is Lactiplantibacillus plantarum, the strain is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, and the preservation unit is North Star Xiya No. 1 and 3 of the Korean region of Beijing. Drawings FIG. 1 shows the results of the oxford cup re-screening transparent circle experiment of the strain producing feruloyl esterase (Lactobacillus plantarum Fs 6), FIG. 2 shows the standard curve of feruloyl content, FIG. 3 shows the growth curve of the strain, FIG. 4 shows the volume increase rate of different yeasts and lactobacillus fermented dough, FIG. 5 shows the strain morphology of Lactobacillus plantarum Fs6, FIG. 6 shows the phylogenetic tree of Lactobacillus plantarum Fs6, and FIG. 7 shows the graph of the bile salt tolerance test result. Detailed Description For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments, and all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments of the present invention are all within the scope of protection of the present invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belong