CN-122012325-A - New Burkholderia species and application thereof in tobacco growth promotion

Abstract

The invention discloses a novel burkholderia species and application thereof in tobacco growth promotion, and belongs to the technical field of microorganisms. The new strain is preserved in China Center for Type Culture Collection (CCTCC), the preservation address is China university of Wuhan, the preservation number is CCTCC NO: M20252947, and the preservation time is 2025, 12 months and 18 days. The invention firstly separates and obtains a new Burkholderia sp BP5, and the new Burkholderia sp BP5 has better phosphate solubilizing capability, promotes seed germination by obviously improving the germination rate, germination vigor, germination number and root length of seeds, can obviously promote plant growth, and has better control effect on plant pathogenic bacterial diseases such as rice bacterial leaf spot and the like, so the new BP5 has better application prospect.

Inventors

• SUN GUANGWEI
• RAO YONG
• LV SHIYOU
• GONG JIAYI
• XU CHANG
• YE JIAOJIAO
• CHEN ZHENGUO
• ZHANG BIAOMING
• RAO JIAXI
• SUN JINGGUO

Assignees

• 湖北省烟草科学研究院

Dates

Publication Date

20260512

Application Date

20260209

Claims (10)

1. 1. The novel burkholderia species BP5 is characterized in that the preservation number of the novel burkholderia species BP5 is CCTCC NO: M20252947.
2. 2. A biological agent comprising the novel burkholderia species BP5 of claim 1.
3. 3. The biological agent of claim 2, wherein the biological agent is a liquid bacterial agent.
4. 4. A method for preparing the biological agent according to any one of claims 2 to 3, which comprises the steps of inoculating the novel species BP5 of Burkholderia according to claim 1 into a liquid culture medium for culturing, and obtaining the biological agent by separating the obtained culture and then re-suspending the culture with a solvent.
5. 5. The use of the novel species BP5 of burkholderia as claimed in claim 1, the biological agent as claimed in any one of claims 2 to 3 or the biological agent obtained by the process of preparation as claimed in claim 4 for the phosphate dissolution.
6. 6. Use of the new species BP5 of burkholderia as claimed in claim 1, the biological agent of any one of claims 2 to 3 or the biological agent obtained by the preparation method of claim 4 for promoting seed germination.
7. 7. The use of claim 6, wherein the seed comprises a cured tobacco seed.
8. 8. The use of the novel species BP5 of burkholderia as claimed in claim 1, the biological agent as claimed in any one of claims 2 to 3 or the biological agent obtained by the preparation process as claimed in claim 4 for plant growth promotion.
9. 9. The use of claim 8, wherein the plant comprises tobacco.
10. 10. The use of the novel species BP5 of burkholderia as claimed in claim 1, the biological agent as claimed in any one of claims 2 to 3 or the biological agent obtained by the preparation method as claimed in claim 4 for controlling plant diseases.

Description

New Burkholderia species and application thereof in tobacco growth promotion Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a novel burkholderia species and application thereof in tobacco growth promotion. Background Phosphate-solubilizing microorganisms (PSMs) are a category of plant rhizosphere growth promoting bacteria (PGPR) with great development potential, and generally refer to a microorganism general term capable of converting insoluble inorganic phosphorus (such as Ca-P, fe-P, al-P) and organic phosphorus (such as phytic acid and phospholipids) in soil into available phosphorus (H 2PO4-/HPO42-) which can be directly absorbed and utilized by plants through mechanisms such as acidification, chelation, oxidation reduction or enzymatic reaction. In the growth and propagation process, PSMs secrete organic acid (such as gluconic acid, citric acid, oxalic acid and the like) to reduce rhizosphere pH and promote phosphate rock to dissolve, and synthesize extracellular enzymes such as acid/alkaline phosphatase, phytase and the like to mineralize organic phosphorus compounds. In addition, PSMs exhibit a typical dual function of "promoting growth-phosphate dissolution" by stimulating plant lateral root and root hair development, enhancing nutrient absorption efficiency, and the like by producing phytohormones such as indole-3-acetic acid (IAA), cytokinins (CKs), and 1-aminocyclopropane-1-carboxylic acid deaminase (ACC DEAMINASE). In recent years, the research of PSMs has been expanded from a single phosphate solubilizing function to multidimensional fields such as heavy metal contaminated soil restoration, bacterial fertilizer carrier innovation, rhizosphere microbiome regulation and control and the like. Studies have shown that Burkholderia sp.can immobilize Cd 2+、Pb2+ by a mechanism of bioprecipitation and bioadsorption, reducing its bioavailability. Meanwhile, the environment-friendly bacterial fertilizer prepared by embedding the phosphate-dissolving bacteria in the sodium alginate-biochar composite microspheres improves the phosphate fertilizer utilization rate by 31.5% and the seed yield by 18.4% in corn field experiments. In addition, the single-cell Raman-heavy water marking technology is utilized by the national institute of sciences of China for urban environment, active phosphate solubilizing bacteria are identified and quantified in situ in 9 different soil types, the remarkable influence of soil properties and fertilization management on PSMS activity is revealed, and a theoretical basis is provided for accurately applying microbial fertilizers. Phosphorus is an element necessary for plant growth, and directly determines plant productivity and ecological function. The total phosphorus content of the soil is about (0.05-3.00) g/kg, but most phosphorus exists mainly in the form of insoluble organic phosphorus or inorganic phosphorus, which limits the direct absorption and utilization of phosphorus by plants, and the effective phosphorus content which can be directly absorbed and utilized by plants is low. Thus, increasing the effective phosphorus content of soil by applying phosphate fertilizer is currently the primary means of agricultural production. However, excessive fertilization may result in a decrease in fertilizer utilization. Therefore, developing new species of efficient phosphate-solubilizing bacteria has important significance for enriching the species of phosphate-solubilizing bacteria and expanding new applications of strains. Disclosure of Invention The invention aims to provide a novel burkholderia strain and application thereof in tobacco growth promotion. The novel burkholderia BP5 provided by the invention has better capabilities of phosphate solubilizing, seed germination promoting and plant growth promoting, and has better control effects on plant pathogenic bacterial diseases such as rice bacterial leaf streak disease, and therefore, has better application prospects in aspects of plant growth promoting and/or plant pathogenic bacterial disease control. In a first aspect, the invention provides a new species of Burkholderia (Burkholderia sp.) BP5, wherein the collection number of the new species of Burkholderia (Burkholderia sp.) BP5 is CCTCC NO: M20252947. The new strain BP5 was isolated from rhizosphere soil from the Bai Yangba Zhenyan field laboratory base (30℃17'37.5 "N, 108℃55' 56.3" E) from Enshi family Miao, hubei province. The colony of the new strain BP5 on the solid culture medium plate is milky white, has raised surface and regular edge, and SEM test shows that the new strain BP5 has typical short rod shape with long axis diameter of 1.5-3.0 microns, short axis diameter of 0.5-0.7 microns and length-diameter ratio of 3.0-4.0, smooth cell wall surface and no obvious flagella or cilia structure. Further, the new strain BP5 is subjected to 16S rDNA sequencing, the 16S rDNA sequences of the new strain BP5 are s