CN-122012327-A - Flavobacterium columniformis NY-1 and application thereof in preventing and controlling microcystis water bloom

Abstract

The invention relates to a Flavobacterium columnar NY-1 and application thereof in preventing and treating microcystis water bloom, belonging to the technical field of microorganisms, wherein the Flavobacterium columnar (Flavobacterium columnare) NY-1 is preserved in the microorganism strain preservation center of Guangdong province at 2 months 11 days of 2026, the preservation number is GDMCC 67866, the device can efficiently and specifically target microcystis colony structure and drive the microcystis colony structure to complete the sedimentation-extinction process, and after the device is applied to a natural water body for 72 hours, the algae inhibition rate is up to 99%, and the microcystis in the water body is almost completely removed, so that the microcystis water bloom is efficiently treated.

Inventors

• DAI WEI
• BI XIANGDONG
• ZHANG DAJUAN
• DONG SHAOJIE
• YING XIAOCHUAN

Assignees

• 天津农学院
• 天津市韵尼科技有限公司

Dates

Publication Date

20260512

Application Date

20260212

Claims (8)

1. 1. A strain of Flavobacterium columnar NY-1, wherein the Flavobacterium columnar (Flavobacterium columnare) NY-1 is preserved in the microorganism strain preservation center of Guangdong province at 2026, 2 nd month and 11 days, and the address is building 5, no. 59 of Dai 100 in Guangzhou city martyr, and the preservation number is GDMCC 67866.
2. 2. The method for culturing flavobacterium columniform NY-1 according to claim 1, comprising the steps of: (1) Inoculating Flavobacterium columniformis NY-1 to an R2A solid culture medium, and performing activation culture at 28+/-2 ℃ to obtain an activated strain; (2) Inoculating the activated strain in the step (1) into an R2A liquid culture medium, and carrying out shake culture at 180-200 rpm and 28+/-2 ℃ to obtain an activated bacterial liquid; (3) Inoculating the activated bacterial liquid in the step (2) into an R2A liquid culture medium according to the inoculum size of 5-10% by volume percentage, and carrying out shake culture at 180-200 rpm and 28+/-2 ℃ to obtain flavobacterium columniform NY-1 bacterial liquid.
3. 3. The use of flavobacterium columniform NY-1 according to claim 1 for controlling microcystis water bloom.
4. 4. The use according to claim 3, wherein the dominant species of microcystis water bloom microcystis and microcystis aeruginosa.
5. 5. The method according to claim 3, wherein the flavobacterium columniform NY-1 is applied to the microcystis water bloom aggregation area in natural water.
6. 6. The use of claim 5, wherein the natural body of water is a crustacean aquaculture pond.
7. 7. The method according to claim 5, wherein the amount is such that the concentration of bacteria in the body of water reaches 10 6 CFU/L.
8. 8. A microbial algistat, comprising Flavobacterium columniform NY-1 according to claim 1.

Description

Flavobacterium columniformis NY-1 and application thereof in preventing and controlling microcystis water bloom Technical Field The invention relates to the technical field of microorganisms, in particular to a flavobacterium columniform NY-1 and application thereof in preventing and controlling microcystis water bloom. Background Microcystis bloom is one of the most common and serious ecological disasters in eutrophic fresh water bodies. In natural water, the microcystis mainly in the form of multicellular aggregated colony, and the colony structure not only provides a physical barrier for the microcystis and can resist ingestion of zooplankton and partial environmental stress, but also obviously enhances the buoyancy of algae cells in the water, and is a key ecological strategy that the microcystis can be maintained on the surface layer of the water for a long time, and forms and sustains water bloom. For example, the document "mechanism for analyzing the formation of microcystis aeruginosa population by using environmental factors to mediate cell surface characteristics" describes in detail that the population can enhance the adaptability of microcystis aeruginosa cells to adverse environments, and the document "bacterial colony change during the growth and disintegration of microcystis aeruginosa (Microcystis aeruginosa) population" also describes the change of bacterial colony during the formation of microcystis aeruginosa population. The natural decay of microcystis bloom often begins with the population losing its stability in the body of water, eventually settling and dying. However, in the field of microbial algae control, most of the current research has focused on microcystis directed to the free, single-cell state. For example, the tested algae strains in the literature of separation, identification and algae dissolving effect comparison of algae dissolving bacteria of different sources, screening separation of algae inhibiting bacteria of south Taihu lake water body and identification of active ingredients thereof, and separation and identification of algae inhibiting bacteria in south America white shrimp culture water body are unicellular microcystis cultured in a laboratory, and the reported algae dissolving bacteria realize the lysis or killing of unicellular microcystis mainly through the modes of secretion of extracellular enzymes or direct contact and the like. However, such research results based on the single cell model face significant application bottlenecks in coping with actual microcystis water bloom, one of the important reasons being that microcystis forming water bloom in natural water body does not exist in a single cell form but gathers and floats in a group form. The document "the effect of pseudomonas A2 on promoting the aggregation and growth of microcystis aeruginosa" also mentions that the microcystis is not easy to exhibit aggregation driving under laboratory culture conditions. Researchers transfer laboratory-amplified unicellular algae to natural eutrophic water for in-situ re-culture, but find that the intercellular aggregation behavior is still obviously limited, and the original population state is difficult to recover. It can be seen that single cell microcystis under laboratory culture conditions is significantly different from the microcystis population in natural water. The strong physical barrier formed by the colony structure has remarkable advantages for the microcystis to resist the external environmental stress, so that the microcystis population which forms water bloom is treated, and the technical difficulty and the complexity are different from those of treating dispersed single cells. In literature 'study of physiological and ecological effects and action mechanisms of algicidal bacteria on algae', authors access composite bacterial liquid containing bacillus cereus DC22, flavobacterium finophilum DC-P and budding yeast into a surrounding water body of a Dian pond to carry out algicidal experiments, and the results of the algicidal experiments show that the transparency of the water body is obviously improved about 40 days after the composite bacterial liquid is accessed. The algae dissolving experiment is performed on microcystis water bloom in natural water, but the algae dissolving period is as long as one month or even more than three months, and the algae dissolving effect is not continuous. In addition, it is worth noting that the prior research of the document proves that the strain DC22 and the strain DC-P have certain inhibition effect on single-cell microcystis under laboratory culture conditions, but in the actual water bloom treatment process, a single strain is not adopted, a composite microbial inoculum is adopted, and budding yeast is introduced as an auxiliary strain. This may be limited by the dependence of the strains DC22 and DC-P on natural conditions such as temperature, light, pH, etc., and by factors such as different complexity