CN-122012328-A - Rhizobium cantaloupe APHBXY-23-1 with functions of promoting growth and inhibiting aspergillus flavus and application thereof

Abstract

The invention discloses a Guangzhou slow rooting tumor bacterium (Bradyrhizobium guangzhouense) APHBXY-23-1 with high-efficiency growth promotion and high-efficiency aspergillus flavus inhibition functions and application thereof, and the preservation number of the strain is CCTCC M20253048. The invention not only maintains the core function of the strain as rhizobia for high-efficiency symbiotic nitrogen fixation and crop growth promotion, but also reveals that the strain can obviously inhibit the growth of aspergillus flavus by releasing volatile substances dimethylbutyric acid for the first time. The discovery breaks through the technical limitations that the traditional rhizobia only has the growth promoting function and lacks the biocontrol function, integrates the core functions of two large agricultural microorganisms of nitrogen fixation and growth promotion and biocontrol bacteriostasis into the same microorganism carrier for the first time, successfully builds a novel rhizobia resource with double functions, creates a novel rhizobia species with biocontrol function, and has important innovation values in theory and application.

Inventors

• LI PEIWU
• ZHANG QI
• ZHOU YANG

Assignees

• 中国农业科学院油料作物研究所

Dates

Publication Date

20260512

Application Date

20260212

Claims (6)

1. 1. The Guangzhou slow-growing rhizobia APHBXY-23-1 with the functions of promoting growth and inhibiting aspergillus flavus is characterized by being classified and named Bradyrhizobium guangzhouense, having a preservation number of CCTCC M20253048, a preservation date of 2025 and 12 months and 30 days, and being preserved in China center for type culture collection, wherein the preservation unit address is in eight-path 299-number Wuhan universities in Wuchang district of Wuhan, hubei province.
2. 2. A microbial agent or formulation comprising at least one of the xanthomonas guangzhou APHBXY-23-1, a fermentation product thereof, or a culture thereof of claim 1, wherein the fermentation product comprises a fermentation broth, a sterile supernatant, or an active substance extracted from the fermentation broth or the sterile supernatant, and wherein the culture comprises a solid culture, a liquid culture, or a dried formulation thereof.
3. 3. Use of the microbial agent or formulation of claim 1, said 3.guangzhou slow rooting tumor cell APHBXY-23-1 or claim 2, for promoting peanut growth and nodulation and nitrogen fixation.
4. 4. Use of the microbial agent or formulation of claim 1, wherein the microbial agent or formulation is selected from the group consisting of a. Cantonensis APHBXY-23-1 and a. Cantonensis, wherein the microbial agent or formulation is selected from the group consisting of a. Cantonensis growth inhibitor, a. Sphaericus production inhibitor and an aflatoxin synthesis inhibitor.
5. 5. The use according to claim 4, wherein said inhibition function is achieved in particular by volatile organic compounds released by said rhizobia cantoniensis APHBXY-23-1.
6. 6. A method for inhibiting Aspergillus flavus is characterized by comprising the step of applying the microbial agent or preparation of the Kwangsania unguiculata APHBXY-23-1 or the microbial agent or preparation of the claim 2 to peanut planting environments or peanut plants.

Description

Rhizobium cantaloupe APHBXY-23-1 with functions of promoting growth and inhibiting aspergillus flavus and application thereof Technical Field The invention belongs to the technical field of biology, and particularly relates to a Guangzhou slow-growing rhizobia APHBXY-23-1 with functions of promoting growth and inhibiting aspergillus flavus and application thereof. Background Currently, in peanut production practice, the improvement of yield and control of aflatoxin contamination generally rely on two independent technical routes: 1. The yield-increasing technology path mainly depends on plant rhizosphere growth-promoting bacteria (PGPR) inoculants. Common bacteria such as azotobacter, phosphorus-dissolving bacteria, bacillus and pseudomonas which secrete phytohormone. The microbial inoculum promotes plant growth through mechanisms such as nitrogen fixation, phosphorus release, synthesis of growth regulating substances and the like, and belongs to the field of biological fertilizers with wide application. 2. The aflatoxin prevention and control technical path mainly comprises 1) agricultural management measures such as selection of resistant varieties, rotation, timely harvest and the like, the effect of which is obviously restricted by the environment, 2) physical and chemical methods such as irradiation, chemical fumigation and adsorbent use, which are mostly used for postpartum treatment, and the problems of high cost, residual risk, quality influence and the like, and 3) biological prevention and control methods, namely application of microbial antagonistic bacteria such as non-toxic aspergillus flavus, bacillus, pseudomonas, trichoderma and the like, and inhibition of aspergillus flavus growth and toxigenic by means of competition, antagonism or resistance induction and the like, are current research hot spots. However, the two types of technologies described above have the following systematic limitations in dealing with the dual needs of peanut "stimulation" and "safeguarding": Single function and synergetic deletion. The existing PGPR microbial inoculum generally lacks the capability of directly inhibiting aspergillus flavus, and the biocontrol microbial inoculum has no substantial contribution to core yield increasing ways such as peanut symbiotic nitrogen fixation and the like. Two types of products are usually required to be applied in production, so that the cost and the operation complexity are increased, and the unstable effect is more likely to be caused by the interaction of strains. The technology is split, and an integrated solution is not formed. The yield and the toxicity prevention are separated for a long time in the technical research and development and application, and a technical system capable of integrating the functions of nitrogen fixation, growth promotion and biocontrol in the same microorganism carrier is lacking. Simple microbial inoculum compounding is difficult to realize stable function coordination in a field environment. The pertinence of the yield-increasing mechanism for leguminous crops is insufficient. Peanut as leguminous crop has its key yield increasing way dependent on efficient root nodule symbiotic nitrogen fixation. The general PGPR can not replace or optimize the specific process, but the traditional rhizobia agents generally have no capability of preventing and controlling aspergillus flavus, so that the yield is increased and the toxicity is prevented from being disjointed on the biological mechanism. The existing prevention and control means have physical and legal limitations. The biological characteristics of the peanuts which bloom on the ground and bear fruits underground make the prevention and control of the key pod bearing period difficult, the coating drug effect is difficult to last to the fruiting period in the sowing period, the foliar application of the pesticide in the fruiting period cannot act on pods in soil, the micro-ecology of the soil is destroyed and the pesticide resistance is easy to cause in the drip irrigation application, and the storage period depends on physical color selection or chemical fumigation, so that the cost is high and the food safety regulation is strictly limited. The pollution source is controlled poorly. Most of the prior art focuses on post-partum treatment or post-infection intervention, and fails to effectively inhibit colonization and development of aspergillus flavus from field sources, particularly rhizosphere microenvironments. The microbial solution capable of synchronously realizing growth promotion, nitrogen fixation and preventive bacteriostasis in the whole peanut growth process is lacking. Therefore, a microbial strain which can effectively promote nitrogen fixation of peanut symbiosis and has a strong aspergillus flavus inhibiting function is needed in the field, so that the dual purposes of yield increase and toxicity prevention are realized in the same biological carrier, and the bottleneck of the