CN-122012329-A - Yuanmingyuan slow-growing rhizobium ASHBWH-23-9 with functions of promoting growth and inhibiting aspergillus flavus and application thereof

Abstract

The invention discloses a garden slow rooting tumor bacterium (Bradyrhizobium yuanmingense) ASHBWH-23-9 with functions of promoting growth and inhibiting aspergillus flavus and application thereof, and the preservation number is CCTCC M20253053. The invention has the innovation that the dual functions of generating cyclopentanone antibacterial volatile matters and efficiently symbiotic nitrogen fixation are integrated in the low rooting tumor bacteria in the garden for the first time. The molecular mechanism is that the volatile substances generated by the method can accurately down regulate the expression of toxin synthesis key genes and spore development core genes in the aspergillus flavus. Compared with the existing microbial inoculum with single function, the microbial inoculum can synchronously realize the promotion of nodulation and nitrogen fixation and the source inhibition of aspergillus flavus in the rhizosphere of peanuts through one-time inoculation, and particularly, the microbial inoculum can continuously play a role in bacteriostasis and gas defense in soil in the pod development period aiming at the characteristics of 'aboveground flowering and underground fruiting' of peanuts.

Inventors

• LI PEIWU
• ZHANG QI
• ZHOU YANG

Assignees

• 中国农业科学院油料作物研究所

Dates

Publication Date

20260512

Application Date

20260212

Claims (6)

1. 1. The Yuanming garden slow-growing rhizobia ASHBWH-23-9 with the functions of promoting growth and inhibiting aspergillus flavus is characterized by being classified and named Bradyrhizobium yuanmingense, having a preservation number of CCTCC M20253053, a preservation date of 2025 and 12 months and 30 days, and being preserved in China center for type culture collection, wherein the preservation unit address is eight paths 299 of Wuchang district in Wuhan, hubei province.
2. 2. A microbial agent or formulation comprising at least one of the rhizobium toruloides ASHBWH-23-9, a fermentation product thereof, or a culture thereof of claim 1, wherein the fermentation product comprises a fermentation broth, a sterile supernatant, or an active substance extracted from the fermentation broth or the sterile supernatant, and wherein the culture comprises a solid culture, a liquid culture, or a dried formulation thereof.
3. 3. Use of a microbial agent or formulation according to claim 1, or claim 2, of a bradyrhizobium torum ASHBWH-23-9 for promoting peanut growth and nodulation and nitrogen fixation.
4. 4. Use of a microbial agent or formulation according to claim 1, or claim 2, of a bradyrhizobium toruloides ASHBWH-23-9 for inhibiting aspergillus flavus growth, spore production and aflatoxin synthesis.
5. 5. The use according to claim 4, characterized in that said inhibition function is achieved in particular by volatile organic compounds released by said bradyrhizobium toruloides ASHBWH-23-9.
6. 6. A method for inhibiting Aspergillus flavus, which is characterized by comprising the step of applying the Rhizobium toruloides ASHBWH-23-9 described in claim 1 or the microbial agent or preparation described in claim 2 to peanut planting environments or peanut plants.

Description

Yuanmingyuan slow-growing rhizobium ASHBWH-23-9 with functions of promoting growth and inhibiting aspergillus flavus and application thereof Technical Field The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a Rhizobium japonicum ASHBWH-23-9 with high-efficiency growth-promoting and aspergillus flavus inhibiting functions and application thereof. Background Peanut as an important oil and commercial crop, its sustainable production faces two major core challenges, continuous yield increase and aflatoxin pollution control. Currently, two parallel technical paths are mainly relied on in the industry: The growth promoting and yield increasing technology mainly realizes nonspecific growth promotion by applying universal plant rhizosphere growth promoting bacteria (PGPR, such as bacillus, pseudomonas and the like), but the microbial inoculum can not effectively activate a root nodule symbiotic nitrogen fixation system specific to leguminous crops, and the yield increasing effect has biological bottleneck. The aflatoxin prevention and control technology mainly comprises agricultural management (passive effect), a physicochemical method (high cost and residual risk) and biological control (mainly depends on antagonistic bacteria such as non-toxic aspergillus flavus, bacillus and the like). However, the prior art has the following systematic defects that (1) the function is split and the synergy is lost, the existing growth promoting microbial agents (comprising traditional rhizobia) generally lack the capability of efficiently inhibiting aspergillus flavus, the biocontrol microbial agents lack direct contribution to the symbiotic nitrogen fixation process, and the two agents are easy to generate competitive antagonism by simple compounding and have unstable field effect. (2) The special habit of peanut 'on-ground flower opening and underground fruiting' causes the pods in the key development stage to be deeply buried in soil, so that the traditional foliar application and seed treatment are difficult to form effective protection at a pollution source, namely a soil fruiting layer. (3) The technical source control is blank, and particularly, the integrated biological solution scheme capable of simultaneously realizing 'high-efficiency symbiotic nitrogen fixation' and 'targeted aspergillus flavus inhibition' on a single microbial carrier from the same ecological niche (rhizosphere) is lacking. Aiming at the industrial bottleneck and technical blank, the invention provides an innovative Yuanming garden slow-growing rhizobia ASHBWH-23-9. The strain integrates the dual functions of high-efficiency symbiotic nitrogen fixation capability and direct aspergillus flavus inhibition by generating specific antibacterial volatile matters (cyclopentanone) in the species for the first time. The method has the innovative significance that nitrogen nutrient supply and disease source prevention and control can be synchronously realized on the rhizosphere of the peanuts through one-time inoculation, the inherent defects that the prior art is single in function and complex in operation, and underground pods are difficult to effectively protect in a fruiting period are fundamentally overcome, and a brand-new and integrated microbial technical solution is provided for green high-yield and safe production of the peanuts. Disclosure of Invention In order to solve the defects in the prior art, the invention provides a Yuanming garden slow-growing rhizobia ASHBWH-23-9 with functions of promoting growth and inhibiting aspergillus flavus and application thereof. In order to solve the technical problems, the invention provides the following technical scheme: The first purpose of the invention is to provide a Yuanming garden slow-growing rhizobia ASHBWH-23-9 with the functions of promoting growth and inhibiting aspergillus flavus, which is classified and named Bradyrhizobium yuanmingense, the preservation number is CCTCC M20253053, the preservation date is 2025, 12 months and 30 days, and the Yuanming garden slow-growing rhizobia is preserved in China center for type culture collection, and the preservation unit address is in eight-path 299-number Wuhan universities in Wuchang district in Hubei province. The second object of the present invention is to provide a microbial agent or preparation comprising at least one of Rhizobium toruloides ASHBWH-23-9, a fermentation product thereof or a culture thereof, wherein the fermentation product comprises a fermentation broth, a sterile supernatant or an active substance extracted from the fermentation broth or the sterile supernatant, and the culture comprises a solid culture, a liquid culture or a dry preparation thereof. The third object of the invention is to provide an application of Rhizobium toruloides ASHBWH-23-9 or a microbial agent or preparation containing Rhizobium toruloides ASHBWH-23-9 in promoting peanut growth and nodulation and nitrog