CN-122012332-A - Rhizobium Anhui ASHBQJ-24-3 with functions of promoting growth and inhibiting aspergillus flavus and application thereof

Abstract

The invention discloses a rhizobium Anhui ASHBQJ-24-3 with functions of promoting growth and inhibiting aspergillus flavus and application thereof, wherein the strain is classified and named Rhizobium anhuiense, and the preservation number is CCTCC M20253051. The invention realizes the integration of the characteristic antibacterial volatile matter of producing phenol and the high-efficiency symbiotic nitrogen fixation on a single strain in rhizobium Anhui for the first time. The mechanism of action is clear, phenol volatile matters generated by the strain can accurately down regulate the expression of toxin synthesis key genes (aflR/aflS) and spore development core genes (abaA/wetA) in the aspergillus flavus. The double targets of growth promotion, nitrogen fixation and source bacteriostasis can be synchronously realized in the peanut rhizosphere-pod micro-domain through one-time inoculation, and particularly, a durable biological fumigation protective layer is constructed in soil in the critical period of pod development aiming at the biological characteristics of peanut 'underground results'.

Inventors

• ZHANG QI
• LI PEIWU
• ZHOU YANG

Assignees

• 中国农业科学院油料作物研究所

Dates

Publication Date

20260512

Application Date

20260212

Claims (6)

1. 1. The rhizobium Anhui ASHBQJ-24-3 with the functions of promoting growth and inhibiting aspergillus flavus is characterized by being classified and named Rhizobium anhuiense, having a preservation number of CCTCC M20253051, a preservation date of 2025 and 12 months and 30 days, and being preserved in China center for type culture collection, wherein the preservation unit address is in eight-path 299-number Wuhan universities in Wuchang district of Wuhan, hubei province.
2. 2. A microbial agent or formulation comprising at least one of rhizobium Anhui ASHBQJ-24-3, a fermentation product thereof, or a culture thereof of claim 1, wherein the fermentation product comprises a fermentation broth, a sterile supernatant, or an active substance extracted from the fermentation broth or sterile supernatant, and wherein the culture comprises a solid culture, a liquid culture, or a dried formulation thereof.
3. 3. Use of the microbial agent or formulation of rhizobia Anhui ASHBQJ-24-3 as defined in claim 1 or claim 2 to promote peanut growth and nodulation and nitrogen fixation.
4. 4. Use of the microbial agent or formulation of rhizobia Anhui ASHBQJ-24-3 as defined in claim 1 or claim 2 to inhibit aspergillus flavus growth, spore production and aflatoxin synthesis.
5. 5. The use according to claim 4, characterized in that said inhibition function is achieved in particular by volatile organic compounds released by said rhizobia Anhui ASHBQJ-24-3.
6. 6. A method for inhibiting Aspergillus flavus, which is characterized by comprising the step of applying the rhizobium Anhui ASHBQJ-24-3 described in claim 1 or the microbial agent or preparation described in claim 2 to peanut planting environments or peanut plants.

Description

Rhizobium Anhui ASHBQJ-24-3 with functions of promoting growth and inhibiting aspergillus flavus and application thereof Technical Field The invention belongs to the technical field of crossing of agricultural microorganisms and plant protection, and particularly relates to rhizobium Anhui (Rhizobium anhuiense) ASHBQJ-24-3 with high-efficiency growth promotion and nitrogen fixation and strong aspergillus flavus inhibition functions and application thereof in green and safe production of peanuts. Research setting 1. Rhizobia growth promoting potential and limitations Rhizobium is a key symbiotic nitrogen fixation partner of leguminous plants, can form rhizobium with a host, and can efficiently convert nitrogen in the atmosphere into nitrogen available to plants, which is the most core yield-increasing mechanism of leguminous crops. In peanut production, inoculation of efficient rhizobia agents is a key green technology for improving yield and reducing fertilizer dependence. However, the traditional rhizobia has relatively single function, mainly focuses on symbiotic nitrogen fixation, and generally lacks direct biocontrol capability on main soil-borne diseases of peanuts, especially aspergillus flavus diseases. 2. Aflatoxin pollution and existing bottleneck prevention and control Aflatoxins (AFs) produced by the infection of peanuts by aspergillus flavus (Aspergillus flavus) are a strong carcinogen, and seriously threaten food safety and industrial economic value. Its contamination begins in the field soil and during the peanut pod development stage, the underground fruit is infested by needles or crevices. The unique habit of "on-ground flowering and underground fruiting" of peanuts makes the traditional chemical prevention and control means (such as leaf spraying) difficult to act on pods in soil in a key infection period, and causes physical prevention and control barriers. The existing field and storage prevention and control technology comprises chemical fumigation, physical adsorption and biocontrol application of non-toxic aspergillus flavus (atoxigenic strains), or focuses on postpartum treatment, or has limited effect, high cost and faces pesticide residue and regulation limit, and is difficult to effectively prevent toxin pollution from the source. 3. Systematic defects and innovation direction of the prior art At present, the yield increase and toxin prevention and control of peanuts depend on two parallel but split technical paths, namely a universal plant rhizosphere growth promoting bacterium (PGPR) or a single-function rhizobium inoculant, focusing on growth promotion or nitrogen fixation, lacking in efficient antibacterial capability, and an antagonistic agent mainly comprising Bacillus spp, pseudomonas spp and the like, focusing on disease prevention and control only and contributing little to the core symbiotic nitrogen fixation process of leguminous crops. The single function results in the need of separately applying two or more products in agricultural production, which not only increases the cost and the labor complexity, but also makes the field effect unstable or even mutually weakened due to the potential competition or antagonism among microorganisms. Therefore, the technical blank to be solved in the field is lacking in a biotechnology scheme capable of integrating the most efficient yield increasing mechanism (symbiotic nitrogen fixation) of leguminous crops and the most critical toxin source prevention and control (targeted aspergillus flavus inhibition) on a single microbial carrier from the same ecological niche (rhizosphere). 4. Innovative positioning of the present invention Based on the above bottleneck, the present invention aims to provide an innovative solution. The novel multifunctional rhizobium strain which has high-efficiency symbiotic nitrogen fixation capacity and can directly and strongly inhibit aspergillus flavus by generating specific antibacterial volatile matters (phenols) is screened and developed, so that the double targets of growth promotion, nitrogen fixation and bacteriostasis and toxicity prevention are synchronously realized in the rhizosphere-pod micro-domain in the same period of peanut growth through one-time inoculation, thereby fundamentally overcoming the defects of functional rupture, temporary cure and temporary cure prevention of the prior art and providing revolutionary microbial resources and technical support for peanut green high-yield and safe production. Disclosure of Invention In order to solve the defects in the prior art, the invention provides rhizobium Anhui ASHBQJ-24-3 with functions of promoting growth and inhibiting aspergillus flavus and application thereof. In order to solve the technical problems, the invention provides the following technical scheme: The first purpose of the invention is to provide a rhizobium Anhui ASHBQJ-24-3 with the functions of promoting growth and inhibiting aspergillus flavus, which is classified and n