CN-122012333-A - Albizia rhizobia APHNZY-24-4 with functions of promoting growth and inhibiting aspergillus flavus and application thereof

Abstract

The invention discloses a rhizobium albizianum (Rhizobium mesosinicum) strain APHNZY-24-4 with functions of promoting peanut growth and inhibiting aspergillus flavus, and the preservation number of the rhizobium albizianum strain is CCTCC M2026228. The invention realizes the integration of the specific antibacterial volatile matter of producing 3-methyl butyric acid and the high-efficiency symbiotic nitrogen fixation on a single strain in the rhizobium albizianum for the first time. The 3-methylbutyric acid released by the strain can accurately down regulate the expression of toxin synthesis key genes and spore development core genes in the aspergillus flavus, thereby realizing efficient bacteriostasis on a molecular level. Through one-time inoculation, the strain can synchronously realize the double aims of promoting growth and fixing nitrogen and inhibiting bacteria from the source in the peanut rhizosphere ecological niche. Especially for the biological characteristics of peanut 'flowering on the ground and fruiting on the ground', the biological fumigation protective layer can be formed in the soil microenvironment in the critical period of pod development.

Inventors

• ZHANG QI
• LI PEIWU
• ZHOU YANG

Assignees

• 中国农业科学院油料作物研究所

Dates

Publication Date

20260512

Application Date

20260212

Claims (6)

1. 1. The rhizobium albizianum APHNZY-24-4 with the functions of promoting growth and inhibiting aspergillus flavus is characterized by being classified and named Rhizobium mesosinicum, having a preservation number of CCTCC M2026228 and a preservation date of 2026, 01 and 23 days, and being preserved in China center for type culture collection, wherein the preservation unit address is in eight-path 299-number Wuhan university schools in Wuchang district in Wuhan, hubei province.
2. 2. A microbial agent or formulation comprising at least one of rhizobium albizianum APHNZY-24-4, a fermentation product thereof, or a culture thereof according to claim 1, wherein the fermentation product comprises a fermentation broth, a sterile supernatant, or an active substance extracted from the fermentation broth or the sterile supernatant, and wherein the culture comprises a solid culture, a liquid culture, or a dried formulation thereof.
3. 3. Use of the microbial agent or formulation of rhizobium albizianum APHNZY-24-4 according to claim 1 or claim 2 for promoting peanut growth and nodulation and nitrogen fixation.
4. 4. Use of the microbial agent or formulation of rhizobium albizianum APHNZY-24-4 according to claim 1 or of the microbial agent or formulation of claim 2 for inhibiting aspergillus flavus growth, spore production and aflatoxin synthesis.
5. 5. The use according to claim 4, wherein said inhibition function is achieved in particular by volatile organic compounds released by said rhizobium albizianum APHNZY-24-4.
6. 6. A method for inhibiting Aspergillus flavus is characterized by comprising the step of applying the rhizobium albizianum APHNZY-24-4 of claim 1 or the microbial agent or preparation of claim 2 to a peanut planting environment or a peanut plant.

Description

Albizia rhizobia APHNZY-24-4 with functions of promoting growth and inhibiting aspergillus flavus and application thereof Technical Field The invention belongs to the technical field of crossing of agricultural microorganisms and plant protection, and particularly relates to rhizobium albizia (Rhizobium mesosinicum) APHNZY-24-4 with high-efficiency growth promotion and nitrogen fixation and strong aspergillus flavus inhibition functions and application thereof in green and safe production of peanuts. Technical Field 1. Importance and core challenges of the peanut industry The peanut is used as an important global oil and economic crop, and the safe production of the peanut has strategic significance for guaranteeing the grain and oil safety and increasing the income of farmers. However, the sustainable production faces two major core challenges, namely continuous yield increase and aflatoxin pollution prevention and control. Aflatoxins (AFs) produced after the peanut is infected by the aspergillus flavus (Aspergillus flavus) are strong cancerogenic substances, seriously endanger human and animal health, and become a key quality safety bottleneck affecting international trade and industrial development. 2. Core mechanism for increasing yield of leguminous crops and effect of rhizobium The peanut is used as leguminous crop, and the most core and most efficient yield increasing way is to establish a specific symbiotic relation with rhizobia to form root nodule, and nitrogen in the atmosphere is converted into nitrogen which can be directly utilized by plants through biological nitrogen fixation. Rhizobium albizianum (Rhizobium mesosinicum) is a rhizobium which is widely existing in soil and can be symbiotic with a plurality of leguminous plants, and has important potential in promoting plant growth and nitrogen fixation. However, the traditional rhizobia agent has single function, mainly focuses on nitrogen fixation and growth promotion, and generally lacks a direct biological control function on peanut aspergillus flavus diseases. 3. Limitations of the existing aflatoxin prevention and control technology At present, the prevention and control of aflatoxin mainly comprises three types of agricultural management, a physicochemical method and biological control. The former two have the problems of passive effect, high cost or chemical residue, and the like, while the main biological control strains (such as Bacillus spp, non-toxic aspergillus flavus, and the like) focus on bacteriostasis, and have no direct contribution to the core yield-increasing mechanism of peanut, namely symbiotic nitrogen fixation, of the specific leguminous crops. It is particularly notable that the unique growth habit of peanuts such as "flowering on the ground and fruiting underground" results in the difficulty of traditional above-ground-based chemical or biological control means to directly act on fruits in the soil during the critical period of pod development, forming a huge gap and physical barrier for control. 4. Systematic drawbacks of prior art paths The existing technological paths of yield increase and gas defense are split into two independent systems, leading to the following systematic defects: (1) The functional singleness and the synergistic effect are lost, the growth promoting microbial inoculum (comprising traditional rhizobium) generally lacks effective inhibition capability for aspergillus flavus, and the biological control microbial inoculum has little contribution to promoting the symbiotic nitrogen fixation process of peanuts. Simple compounding of the two often results in unstable effects and even mutual weakening due to competition or antagonism between microorganisms. (2) The operation is complex and the cost is high, namely two types of products are required to be purchased, stored and applied respectively, and the production cost, the labor investment and the management complexity are obviously increased. (3) The source control is weak, and the integrated biotechnology scheme for synchronously realizing growth promotion and nitrogen fixation and source aspergillus flavus inhibition in the same growth period through the same microorganism carrier from the same ecological niche (rhizosphere) in the peanut growth season is lacking. 5. The invention is innovative positioning and technical blank Based on the industrial bottleneck and the state of the art, a clear technical blank exists in the field, and no public report or commercial application shows that the rhizobia albicans (Rhizobium mesosinicum) has a multifunctional strain which can effectively inhibit aspergillus flavus through producing specific antibacterial volatile substances (such as 3-methylbutyric acid) and maintain the high-efficiency symbiotic nitrogen fixation capability. The invention aims to fill the gap, and synchronously regulates and controls the core yield increasing mechanism (symbiotic nitrogen fixation) and the key toxin pollution source